Cargando…

A point mutation in MC06g1112 encoding FLOWERING LOCUS T decreases the first flower node in bitter gourd (Momordica charantia L.)

In Cucurbitaceae crops, the first flower node (FFN) is an important agronomic trait which can impact the onset of maturity, the production of female flowers, and yield. However, the gene responsible for regulating FFN in bitter gourd is unknown. Here, we used a gynoecious line (S156G) with low FFN a...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhong, Jian, Cui, Junjie, Miao, Mingjun, Hu, Fang, Dong, Jichi, Liu, Jia, Zhong, Chunfeng, Cheng, Jiaowen, Hu, Kailin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10595031/
https://www.ncbi.nlm.nih.gov/pubmed/37881613
http://dx.doi.org/10.3389/fpls.2023.1153208
_version_ 1785124776695562240
author Zhong, Jian
Cui, Junjie
Miao, Mingjun
Hu, Fang
Dong, Jichi
Liu, Jia
Zhong, Chunfeng
Cheng, Jiaowen
Hu, Kailin
author_facet Zhong, Jian
Cui, Junjie
Miao, Mingjun
Hu, Fang
Dong, Jichi
Liu, Jia
Zhong, Chunfeng
Cheng, Jiaowen
Hu, Kailin
author_sort Zhong, Jian
collection PubMed
description In Cucurbitaceae crops, the first flower node (FFN) is an important agronomic trait which can impact the onset of maturity, the production of female flowers, and yield. However, the gene responsible for regulating FFN in bitter gourd is unknown. Here, we used a gynoecious line (S156G) with low FFN as the female parent and a monoecious line (K8-201) with high FFN as the male parent to obtain F(1) and F(2) generations. Genetic analysis indicated that the low FFN trait was incompletely dominant over the high FFN trait. A major quantitative trait locus (QTL)-Mcffn and four minor effect QTLs-Mcffn1.1, Mcffn1.2, Mcffn1.3, and Mcffn1.4 were detected by whole-genome re-sequencing-based QTL mapping in the S156G×K8-201 F(2) population (n=234) cultivated in autumn 2019. The Mcffn locus was further supported by molecular marker-based QTL mapping in three S156G×K8-201 F(2) populations planted in autumn 2019 (n=234), autumn 2020 (n=192), and spring 2022 (n=205). Then, the Mcffn locus was fine-mapped into a 77.98-kb physical region on pseudochromosome MC06 using a large S156G×K8-201 F(2) population (n=2,402). MC06g1112, which is a homolog of FLOWERING LOCUS T (FT), was considered as the most likely Mcffn candidate gene according to both expression and sequence variation analyses between parental lines. A point mutation (C277T) in MC06g1112, which results in a P93S amino acid mutation between parental lines, may be responsible for decreasing FFN in bitter gourd. Our findings provide a helpful resource for the molecular marker-assisted selective breeding of bitter gourd.
format Online
Article
Text
id pubmed-10595031
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-105950312023-10-25 A point mutation in MC06g1112 encoding FLOWERING LOCUS T decreases the first flower node in bitter gourd (Momordica charantia L.) Zhong, Jian Cui, Junjie Miao, Mingjun Hu, Fang Dong, Jichi Liu, Jia Zhong, Chunfeng Cheng, Jiaowen Hu, Kailin Front Plant Sci Plant Science In Cucurbitaceae crops, the first flower node (FFN) is an important agronomic trait which can impact the onset of maturity, the production of female flowers, and yield. However, the gene responsible for regulating FFN in bitter gourd is unknown. Here, we used a gynoecious line (S156G) with low FFN as the female parent and a monoecious line (K8-201) with high FFN as the male parent to obtain F(1) and F(2) generations. Genetic analysis indicated that the low FFN trait was incompletely dominant over the high FFN trait. A major quantitative trait locus (QTL)-Mcffn and four minor effect QTLs-Mcffn1.1, Mcffn1.2, Mcffn1.3, and Mcffn1.4 were detected by whole-genome re-sequencing-based QTL mapping in the S156G×K8-201 F(2) population (n=234) cultivated in autumn 2019. The Mcffn locus was further supported by molecular marker-based QTL mapping in three S156G×K8-201 F(2) populations planted in autumn 2019 (n=234), autumn 2020 (n=192), and spring 2022 (n=205). Then, the Mcffn locus was fine-mapped into a 77.98-kb physical region on pseudochromosome MC06 using a large S156G×K8-201 F(2) population (n=2,402). MC06g1112, which is a homolog of FLOWERING LOCUS T (FT), was considered as the most likely Mcffn candidate gene according to both expression and sequence variation analyses between parental lines. A point mutation (C277T) in MC06g1112, which results in a P93S amino acid mutation between parental lines, may be responsible for decreasing FFN in bitter gourd. Our findings provide a helpful resource for the molecular marker-assisted selective breeding of bitter gourd. Frontiers Media S.A. 2023-10-10 /pmc/articles/PMC10595031/ /pubmed/37881613 http://dx.doi.org/10.3389/fpls.2023.1153208 Text en Copyright © 2023 Zhong, Cui, Miao, Hu, Dong, Liu, Zhong, Cheng and Hu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhong, Jian
Cui, Junjie
Miao, Mingjun
Hu, Fang
Dong, Jichi
Liu, Jia
Zhong, Chunfeng
Cheng, Jiaowen
Hu, Kailin
A point mutation in MC06g1112 encoding FLOWERING LOCUS T decreases the first flower node in bitter gourd (Momordica charantia L.)
title A point mutation in MC06g1112 encoding FLOWERING LOCUS T decreases the first flower node in bitter gourd (Momordica charantia L.)
title_full A point mutation in MC06g1112 encoding FLOWERING LOCUS T decreases the first flower node in bitter gourd (Momordica charantia L.)
title_fullStr A point mutation in MC06g1112 encoding FLOWERING LOCUS T decreases the first flower node in bitter gourd (Momordica charantia L.)
title_full_unstemmed A point mutation in MC06g1112 encoding FLOWERING LOCUS T decreases the first flower node in bitter gourd (Momordica charantia L.)
title_short A point mutation in MC06g1112 encoding FLOWERING LOCUS T decreases the first flower node in bitter gourd (Momordica charantia L.)
title_sort point mutation in mc06g1112 encoding flowering locus t decreases the first flower node in bitter gourd (momordica charantia l.)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10595031/
https://www.ncbi.nlm.nih.gov/pubmed/37881613
http://dx.doi.org/10.3389/fpls.2023.1153208
work_keys_str_mv AT zhongjian apointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT cuijunjie apointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT miaomingjun apointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT hufang apointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT dongjichi apointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT liujia apointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT zhongchunfeng apointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT chengjiaowen apointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT hukailin apointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT zhongjian pointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT cuijunjie pointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT miaomingjun pointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT hufang pointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT dongjichi pointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT liujia pointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT zhongchunfeng pointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT chengjiaowen pointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial
AT hukailin pointmutationinmc06g1112encodingfloweringlocustdecreasesthefirstflowernodeinbittergourdmomordicacharantial