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Viricide activity of antimicrobial blue light (405 nm) against the SARS-CoV-2 and influenza virus
BACKGROUND: Over the years, antimicrobial blue light (aBL) at a 405 nm wavelength has emerged as a potential alternative treatment for reducing the environmental contamination. It is well accepted that aBL is much less detrimental to host cells than UV-C irradiation and can be used in the presence o...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10596482/ http://dx.doi.org/10.1093/eurpub/ckad160.1044 |
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author | Dragoni, L Amodeo, D Cevenini, G Marchi, S Trombetta, C Nante, N Messina, G |
author_facet | Dragoni, L Amodeo, D Cevenini, G Marchi, S Trombetta, C Nante, N Messina, G |
author_sort | Dragoni, L |
collection | PubMed |
description | BACKGROUND: Over the years, antimicrobial blue light (aBL) at a 405 nm wavelength has emerged as a potential alternative treatment for reducing the environmental contamination. It is well accepted that aBL is much less detrimental to host cells than UV-C irradiation and can be used in the presence of people. This study aims to determine the viricide activity, against the SARS-CoV-2 and influenza virus types A and B, of the nUV-A radiation, using a customized nUV-A ceiling lamp System. METHODS: The experiment took place in March 2023. An nUV-A ceiling lamp prototype, composed of 9 LEDs at 405 nm, was fixed on a metal scaffold, on which the outline of a multiwell plate (24 wells) was traced to carry out the tests correctly. Each plate well was inoculated with 100 µL of SARS-CoV2 or influenza virus types A or B suspension. The multiwell plate was irradiated at 32 cm for 45 and 90 minutes, with an emitted energy dose of 12 J/cm2 and 24 J/cm2. Three samples were inoculated with viruses and subjected to the action of nUV-A as per protocol, and 3 samples were inoculated but not treated with nUV-A to determine viral titer after recovery and examined immediately after inoculation. The re-collected suspensions were used to inoculate a wells plate into which the VERO E6 cell cultures were fixed and then incubated for 3 days at 37° C. RESULTS: The results showed that after a 45 min exposition, the measurable log10 mean reduction was 1.50 for SARS-CoV2, 0.13 for influenza virus type A and 0.12 for type B; after 90 min the log10 mean reduction was 2.33 for SARS-CoV2, 0.16 for influenza virus type A and 0.17 for type B. CONCLUSIONS: Tests have shown that aBL can be effective in reducing the viral load of the SARS-CoV2 virus and, to a lesser extent, the influenza A/B viruses. Disinfection with aBL appears effective and avoid the adverse effects of using UV-C. Further studies will evaluate how to improve the results on influenza viruses and the application on other viruses such as RSV. KEY MESSAGES: • NearUV-A lamp has proven to reduce the virucide activity of all viruses, more for sars-cov2. • Using aBL avoids the adverse UV-C’s effects. |
format | Online Article Text |
id | pubmed-10596482 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-105964822023-10-25 Viricide activity of antimicrobial blue light (405 nm) against the SARS-CoV-2 and influenza virus Dragoni, L Amodeo, D Cevenini, G Marchi, S Trombetta, C Nante, N Messina, G Eur J Public Health Poster Displays BACKGROUND: Over the years, antimicrobial blue light (aBL) at a 405 nm wavelength has emerged as a potential alternative treatment for reducing the environmental contamination. It is well accepted that aBL is much less detrimental to host cells than UV-C irradiation and can be used in the presence of people. This study aims to determine the viricide activity, against the SARS-CoV-2 and influenza virus types A and B, of the nUV-A radiation, using a customized nUV-A ceiling lamp System. METHODS: The experiment took place in March 2023. An nUV-A ceiling lamp prototype, composed of 9 LEDs at 405 nm, was fixed on a metal scaffold, on which the outline of a multiwell plate (24 wells) was traced to carry out the tests correctly. Each plate well was inoculated with 100 µL of SARS-CoV2 or influenza virus types A or B suspension. The multiwell plate was irradiated at 32 cm for 45 and 90 minutes, with an emitted energy dose of 12 J/cm2 and 24 J/cm2. Three samples were inoculated with viruses and subjected to the action of nUV-A as per protocol, and 3 samples were inoculated but not treated with nUV-A to determine viral titer after recovery and examined immediately after inoculation. The re-collected suspensions were used to inoculate a wells plate into which the VERO E6 cell cultures were fixed and then incubated for 3 days at 37° C. RESULTS: The results showed that after a 45 min exposition, the measurable log10 mean reduction was 1.50 for SARS-CoV2, 0.13 for influenza virus type A and 0.12 for type B; after 90 min the log10 mean reduction was 2.33 for SARS-CoV2, 0.16 for influenza virus type A and 0.17 for type B. CONCLUSIONS: Tests have shown that aBL can be effective in reducing the viral load of the SARS-CoV2 virus and, to a lesser extent, the influenza A/B viruses. Disinfection with aBL appears effective and avoid the adverse effects of using UV-C. Further studies will evaluate how to improve the results on influenza viruses and the application on other viruses such as RSV. KEY MESSAGES: • NearUV-A lamp has proven to reduce the virucide activity of all viruses, more for sars-cov2. • Using aBL avoids the adverse UV-C’s effects. Oxford University Press 2023-10-24 /pmc/articles/PMC10596482/ http://dx.doi.org/10.1093/eurpub/ckad160.1044 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of the European Public Health Association. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Poster Displays Dragoni, L Amodeo, D Cevenini, G Marchi, S Trombetta, C Nante, N Messina, G Viricide activity of antimicrobial blue light (405 nm) against the SARS-CoV-2 and influenza virus |
title | Viricide activity of antimicrobial blue light (405 nm) against the SARS-CoV-2 and influenza virus |
title_full | Viricide activity of antimicrobial blue light (405 nm) against the SARS-CoV-2 and influenza virus |
title_fullStr | Viricide activity of antimicrobial blue light (405 nm) against the SARS-CoV-2 and influenza virus |
title_full_unstemmed | Viricide activity of antimicrobial blue light (405 nm) against the SARS-CoV-2 and influenza virus |
title_short | Viricide activity of antimicrobial blue light (405 nm) against the SARS-CoV-2 and influenza virus |
title_sort | viricide activity of antimicrobial blue light (405 nm) against the sars-cov-2 and influenza virus |
topic | Poster Displays |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10596482/ http://dx.doi.org/10.1093/eurpub/ckad160.1044 |
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