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A novel method to extend viability and functionality of living heart slices

Living heart slices have recently emerged as a powerful experimental model for fundamental cardiac research. By retaining the structure and function of the native myocardium while maintaining the simplicity of cell culture models, heart slices can be easily employed in electrophysiological, pharmaco...

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Autores principales: Ross, Abigail J., Krumova, Iva, Tunc, Berfin, Wu, Qin, Wu, Changhao, Camelliti, Patrizia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10597746/
https://www.ncbi.nlm.nih.gov/pubmed/37881724
http://dx.doi.org/10.3389/fcvm.2023.1244630
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author Ross, Abigail J.
Krumova, Iva
Tunc, Berfin
Wu, Qin
Wu, Changhao
Camelliti, Patrizia
author_facet Ross, Abigail J.
Krumova, Iva
Tunc, Berfin
Wu, Qin
Wu, Changhao
Camelliti, Patrizia
author_sort Ross, Abigail J.
collection PubMed
description Living heart slices have recently emerged as a powerful experimental model for fundamental cardiac research. By retaining the structure and function of the native myocardium while maintaining the simplicity of cell culture models, heart slices can be easily employed in electrophysiological, pharmacological, biochemical, and structural investigations. One single heart yields many slices (>20 slices for rodents, >100 slices for porcine or human hearts), however due to the low throughput of most assays and rapid slice degeneration within 24 h of preparation, many slices remain unused and are discarded at the end of the preparation day. Here we present a novel method to extend viability and functionality of living heart slices, enabling their use in experiments over several consecutive days following preparation. By combining hypothermic conditions with inhibition of myosin II ATPase using 2,3-butanedione monoxime (BDM), slices prepared from the left ventricle of porcine hearts remain viable and exhibit preserved contractile function and morphology for up to 6 days. Electrophysiological function was also confirmed over the 6 days by extracellular field potentials recordings. This simple method not only maximizes the use of slices prepared from one single heart, thus reducing the number of animals required, but also increases data reproducibility by allowing multiple electrophysiological, pharmacological, biochemical, and structural studies to be performed from the same heart.
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spelling pubmed-105977462023-10-25 A novel method to extend viability and functionality of living heart slices Ross, Abigail J. Krumova, Iva Tunc, Berfin Wu, Qin Wu, Changhao Camelliti, Patrizia Front Cardiovasc Med Cardiovascular Medicine Living heart slices have recently emerged as a powerful experimental model for fundamental cardiac research. By retaining the structure and function of the native myocardium while maintaining the simplicity of cell culture models, heart slices can be easily employed in electrophysiological, pharmacological, biochemical, and structural investigations. One single heart yields many slices (>20 slices for rodents, >100 slices for porcine or human hearts), however due to the low throughput of most assays and rapid slice degeneration within 24 h of preparation, many slices remain unused and are discarded at the end of the preparation day. Here we present a novel method to extend viability and functionality of living heart slices, enabling their use in experiments over several consecutive days following preparation. By combining hypothermic conditions with inhibition of myosin II ATPase using 2,3-butanedione monoxime (BDM), slices prepared from the left ventricle of porcine hearts remain viable and exhibit preserved contractile function and morphology for up to 6 days. Electrophysiological function was also confirmed over the 6 days by extracellular field potentials recordings. This simple method not only maximizes the use of slices prepared from one single heart, thus reducing the number of animals required, but also increases data reproducibility by allowing multiple electrophysiological, pharmacological, biochemical, and structural studies to be performed from the same heart. Frontiers Media S.A. 2023-10-10 /pmc/articles/PMC10597746/ /pubmed/37881724 http://dx.doi.org/10.3389/fcvm.2023.1244630 Text en © 2023 Ross, Krumova, Tunc, Wu, Wu and Camelliti. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Ross, Abigail J.
Krumova, Iva
Tunc, Berfin
Wu, Qin
Wu, Changhao
Camelliti, Patrizia
A novel method to extend viability and functionality of living heart slices
title A novel method to extend viability and functionality of living heart slices
title_full A novel method to extend viability and functionality of living heart slices
title_fullStr A novel method to extend viability and functionality of living heart slices
title_full_unstemmed A novel method to extend viability and functionality of living heart slices
title_short A novel method to extend viability and functionality of living heart slices
title_sort novel method to extend viability and functionality of living heart slices
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10597746/
https://www.ncbi.nlm.nih.gov/pubmed/37881724
http://dx.doi.org/10.3389/fcvm.2023.1244630
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