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Body mass index-dependent immunological profile changes after left ventricular assist device implantation

PURPOSE: Infection is a common complication following left ventricular assist device (LVAD) implantation. Patients with obesity are particularly at risk due to their high percentage of adipose tissue and the resulting chronic inflammatory state and resulting immunological changes. This study investi...

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Autores principales: Klaeske, Kristin, Messer, Eva Katharina, Klein, Sara, Sieg, Franz, Eifert, Sandra, Haunschild, Josephina, Jawad, Khalil, Saeed, Diyar, Dashkevich, Alexey, Borger, Michael A., Dieterlen, Maja-Theresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10597783/
https://www.ncbi.nlm.nih.gov/pubmed/37885885
http://dx.doi.org/10.3389/fimmu.2023.1256725
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author Klaeske, Kristin
Messer, Eva Katharina
Klein, Sara
Sieg, Franz
Eifert, Sandra
Haunschild, Josephina
Jawad, Khalil
Saeed, Diyar
Dashkevich, Alexey
Borger, Michael A.
Dieterlen, Maja-Theresa
author_facet Klaeske, Kristin
Messer, Eva Katharina
Klein, Sara
Sieg, Franz
Eifert, Sandra
Haunschild, Josephina
Jawad, Khalil
Saeed, Diyar
Dashkevich, Alexey
Borger, Michael A.
Dieterlen, Maja-Theresa
author_sort Klaeske, Kristin
collection PubMed
description PURPOSE: Infection is a common complication following left ventricular assist device (LVAD) implantation. Patients with obesity are particularly at risk due to their high percentage of adipose tissue and the resulting chronic inflammatory state and resulting immunological changes. This study investigated changes of immunological parameters in relation to body mass index (BMI) during the first year after LVAD implantation. METHODS: Blood samples were obtained prior to LVAD implantation and at 3 (1(st) FU), 6 (2(nd) FU) and 12 mo (3(rd) FU) after LVAD implantation. Patients were divided into three groups (normal weight: BMI of 18.5-24.9 kg/m(2); n=12; pre-obesity: 25.0-29.9 kg/m(2); n=15; obesity: ≥ 30.0 kg/m(2); n=17) based on their BMI at the time of LVAD implantation. Flow cytometric analyses for CD4(+) and CD8(+) T cells, regulatory T cells (T(regs)), B cells as well as dendritic cells (DCs) were performed. RESULTS: After LVAD implantation, obese patients (0.51 ± 0.20%) showed a higher proportion of overall DCs than normal-weight (0.28 ± 0.10%) and pre-obese patients (0.32 ± 0.11%, p<0.01) at 3(rd) FU. The proportion of BDCA3(+) myeloid DCs was lower in obese patients (64.3 ± 26.5%) compared to normal-weight patients (82.7 ± 10.0%, p(normal-weight vs. obesity)=0.05) at 2(nd) FU after LVAD implantation. The analysis of BDCA4(+) plasmacytoid DCs revealed a reduced proportion in pre-obese (21.1 ± 9.8%, p(normal-weight vs. pre-obesity)=0.01) and obese patients (23.7 ± 10.6%, p(normal-weight vs. obesity)=0.05) compared to normal-weight patients (33.1 ± 8.2%) in the 1(st) FU. T cell analysis showed that CD4(+) T cells of obese patients (62.4 ± 9.0%) significantly increased in comparison to pre-obese patients (52.7 ± 10.0%, p(pre-obesity vs. obesity)=0.05) and CD8(+) T cells were lower in obese patients (31.8 ± 8.5%) than in normal-weight patients (42.4 ± 14.2%; p(normal-weight vs. obesity)=0.04) at the 3(rd) FU. Furthermore, we observed significantly reduced proportions of T(regs) in pre-obese patients compared to normal-weight and obese patients at 2(nd) FU (p=0.02) and 3(rd) FU (p=0.01) after LVAD implantation. CONCLUSION: This study reported changes of the innate and adaptive immune system of pre-obese and obese compared to normal-weight patients one year after LVAD implantation. DCs and their subsets, CD8(+) T cells and T(regs) were affected immune cell populations that indicate immunological changes which might increase the incidence of postoperative infection.
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spelling pubmed-105977832023-10-26 Body mass index-dependent immunological profile changes after left ventricular assist device implantation Klaeske, Kristin Messer, Eva Katharina Klein, Sara Sieg, Franz Eifert, Sandra Haunschild, Josephina Jawad, Khalil Saeed, Diyar Dashkevich, Alexey Borger, Michael A. Dieterlen, Maja-Theresa Front Immunol Immunology PURPOSE: Infection is a common complication following left ventricular assist device (LVAD) implantation. Patients with obesity are particularly at risk due to their high percentage of adipose tissue and the resulting chronic inflammatory state and resulting immunological changes. This study investigated changes of immunological parameters in relation to body mass index (BMI) during the first year after LVAD implantation. METHODS: Blood samples were obtained prior to LVAD implantation and at 3 (1(st) FU), 6 (2(nd) FU) and 12 mo (3(rd) FU) after LVAD implantation. Patients were divided into three groups (normal weight: BMI of 18.5-24.9 kg/m(2); n=12; pre-obesity: 25.0-29.9 kg/m(2); n=15; obesity: ≥ 30.0 kg/m(2); n=17) based on their BMI at the time of LVAD implantation. Flow cytometric analyses for CD4(+) and CD8(+) T cells, regulatory T cells (T(regs)), B cells as well as dendritic cells (DCs) were performed. RESULTS: After LVAD implantation, obese patients (0.51 ± 0.20%) showed a higher proportion of overall DCs than normal-weight (0.28 ± 0.10%) and pre-obese patients (0.32 ± 0.11%, p<0.01) at 3(rd) FU. The proportion of BDCA3(+) myeloid DCs was lower in obese patients (64.3 ± 26.5%) compared to normal-weight patients (82.7 ± 10.0%, p(normal-weight vs. obesity)=0.05) at 2(nd) FU after LVAD implantation. The analysis of BDCA4(+) plasmacytoid DCs revealed a reduced proportion in pre-obese (21.1 ± 9.8%, p(normal-weight vs. pre-obesity)=0.01) and obese patients (23.7 ± 10.6%, p(normal-weight vs. obesity)=0.05) compared to normal-weight patients (33.1 ± 8.2%) in the 1(st) FU. T cell analysis showed that CD4(+) T cells of obese patients (62.4 ± 9.0%) significantly increased in comparison to pre-obese patients (52.7 ± 10.0%, p(pre-obesity vs. obesity)=0.05) and CD8(+) T cells were lower in obese patients (31.8 ± 8.5%) than in normal-weight patients (42.4 ± 14.2%; p(normal-weight vs. obesity)=0.04) at the 3(rd) FU. Furthermore, we observed significantly reduced proportions of T(regs) in pre-obese patients compared to normal-weight and obese patients at 2(nd) FU (p=0.02) and 3(rd) FU (p=0.01) after LVAD implantation. CONCLUSION: This study reported changes of the innate and adaptive immune system of pre-obese and obese compared to normal-weight patients one year after LVAD implantation. DCs and their subsets, CD8(+) T cells and T(regs) were affected immune cell populations that indicate immunological changes which might increase the incidence of postoperative infection. Frontiers Media S.A. 2023-10-10 /pmc/articles/PMC10597783/ /pubmed/37885885 http://dx.doi.org/10.3389/fimmu.2023.1256725 Text en Copyright © 2023 Klaeske, Messer, Klein, Sieg, Eifert, Haunschild, Jawad, Saeed, Dashkevich, Borger and Dieterlen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Klaeske, Kristin
Messer, Eva Katharina
Klein, Sara
Sieg, Franz
Eifert, Sandra
Haunschild, Josephina
Jawad, Khalil
Saeed, Diyar
Dashkevich, Alexey
Borger, Michael A.
Dieterlen, Maja-Theresa
Body mass index-dependent immunological profile changes after left ventricular assist device implantation
title Body mass index-dependent immunological profile changes after left ventricular assist device implantation
title_full Body mass index-dependent immunological profile changes after left ventricular assist device implantation
title_fullStr Body mass index-dependent immunological profile changes after left ventricular assist device implantation
title_full_unstemmed Body mass index-dependent immunological profile changes after left ventricular assist device implantation
title_short Body mass index-dependent immunological profile changes after left ventricular assist device implantation
title_sort body mass index-dependent immunological profile changes after left ventricular assist device implantation
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10597783/
https://www.ncbi.nlm.nih.gov/pubmed/37885885
http://dx.doi.org/10.3389/fimmu.2023.1256725
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