Preliminary Information of Iranian Lizard Leishmania Promastigote Transcriptome Sequencing by Next Generation Sequencing (NGS) Method

BACKGROUND: A lizard Leishmania has been isolated from a lizard (Agama agilis) in Iran. Its genome sequence has not been determined, so far. METHODS: The study was done at Shahid Beheshti University of Medical Sciences, Tehran, Iran in 2017–2023. Leishmania promastigotes were cultured in RPMI(1640)...

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Autores principales: Ehya, Farveh, Kalantari, Sima, Bandehpour, Mojgan, Kazemi, Bahram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2023
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10597888/
https://www.ncbi.nlm.nih.gov/pubmed/37886243
http://dx.doi.org/10.18502/ijpa.v18i3.13759
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author Ehya, Farveh
Kalantari, Sima
Bandehpour, Mojgan
Kazemi, Bahram
author_facet Ehya, Farveh
Kalantari, Sima
Bandehpour, Mojgan
Kazemi, Bahram
author_sort Ehya, Farveh
collection PubMed
description BACKGROUND: A lizard Leishmania has been isolated from a lizard (Agama agilis) in Iran. Its genome sequence has not been determined, so far. METHODS: The study was done at Shahid Beheshti University of Medical Sciences, Tehran, Iran in 2017–2023. Leishmania promastigotes were cultured in RPMI(1640) culture medium and collected at logarithmic phase by centrigugation. Parasite RNA was extracted by the Qiagene standard kit and its quantity and quality was determined and sequenced by NGS method with Illumina PE machine at BGI Company (China). RESULTS: The number of 8316 mRNA, 83 tRNA, 63 rRNA, 83 ncRNA, 5 snRNA, 1039 snoRNA, 36 region, and 3 repeat regions, 8343 CDS, 9597 Exon and 9292 Genes were identified in promastigote of Iranian lizard Leishmania. CONCLUSION: Genomic elements of Iranian lizards Leishmania (with unique characteristics) were determined and identified by NGS system.
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spelling pubmed-105978882023-10-26 Preliminary Information of Iranian Lizard Leishmania Promastigote Transcriptome Sequencing by Next Generation Sequencing (NGS) Method Ehya, Farveh Kalantari, Sima Bandehpour, Mojgan Kazemi, Bahram Iran J Parasitol Original Article BACKGROUND: A lizard Leishmania has been isolated from a lizard (Agama agilis) in Iran. Its genome sequence has not been determined, so far. METHODS: The study was done at Shahid Beheshti University of Medical Sciences, Tehran, Iran in 2017–2023. Leishmania promastigotes were cultured in RPMI(1640) culture medium and collected at logarithmic phase by centrigugation. Parasite RNA was extracted by the Qiagene standard kit and its quantity and quality was determined and sequenced by NGS method with Illumina PE machine at BGI Company (China). RESULTS: The number of 8316 mRNA, 83 tRNA, 63 rRNA, 83 ncRNA, 5 snRNA, 1039 snoRNA, 36 region, and 3 repeat regions, 8343 CDS, 9597 Exon and 9292 Genes were identified in promastigote of Iranian lizard Leishmania. CONCLUSION: Genomic elements of Iranian lizards Leishmania (with unique characteristics) were determined and identified by NGS system. Tehran University of Medical Sciences 2023 /pmc/articles/PMC10597888/ /pubmed/37886243 http://dx.doi.org/10.18502/ijpa.v18i3.13759 Text en © 2023 Ehya et al. Published by Tehran University of Medical Sciences. https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Article
Ehya, Farveh
Kalantari, Sima
Bandehpour, Mojgan
Kazemi, Bahram
Preliminary Information of Iranian Lizard Leishmania Promastigote Transcriptome Sequencing by Next Generation Sequencing (NGS) Method
title Preliminary Information of Iranian Lizard Leishmania Promastigote Transcriptome Sequencing by Next Generation Sequencing (NGS) Method
title_full Preliminary Information of Iranian Lizard Leishmania Promastigote Transcriptome Sequencing by Next Generation Sequencing (NGS) Method
title_fullStr Preliminary Information of Iranian Lizard Leishmania Promastigote Transcriptome Sequencing by Next Generation Sequencing (NGS) Method
title_full_unstemmed Preliminary Information of Iranian Lizard Leishmania Promastigote Transcriptome Sequencing by Next Generation Sequencing (NGS) Method
title_short Preliminary Information of Iranian Lizard Leishmania Promastigote Transcriptome Sequencing by Next Generation Sequencing (NGS) Method
title_sort preliminary information of iranian lizard leishmania promastigote transcriptome sequencing by next generation sequencing (ngs) method
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10597888/
https://www.ncbi.nlm.nih.gov/pubmed/37886243
http://dx.doi.org/10.18502/ijpa.v18i3.13759
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