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Functional assessment of lysosomal Rab7 and RILP with RNA interference and overexpression in Spodoptera frugiperda Sf9 cell lines

The interaction manner and biological function of Rab7 and its effector, Rab-interacting lysosomal protein (RILP), remain unclear in invertebrates. We provide a protocol for detecting the effects of Rab7 and RILP terminals on lysosome and autophagy in Spodoptera frugiperda Sf9 cells with overexpress...

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Detalles Bibliográficos
Autores principales: Cui, Gaofeng, Jiang, Zhiyan, Zhong, Guohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598039/
https://www.ncbi.nlm.nih.gov/pubmed/37851568
http://dx.doi.org/10.1016/j.xpro.2023.102646