Comparison of two lab-scale protocols for enhanced mRNA-based CAR-T cell generation and functionality

Process development for transferring lab-scale research workflows to automated manufacturing procedures is critical for chimeric antigen receptor (CAR)-T cell therapies. Therefore, the key factor for cell viability, expansion, modification, and functionality is the optimal combination of medium and...

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Autores principales: von Auw, Nadine, Serfling, Robert, Kitte, Reni, Hilger, Nadja, Zhang, Chengkang, Gebhardt, Clara, Duenkel, Anna, Franz, Paul, Koehl, Ulrike, Fricke, Stephan, Tretbar, U. Sandy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598065/
https://www.ncbi.nlm.nih.gov/pubmed/37875523
http://dx.doi.org/10.1038/s41598-023-45197-x
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author von Auw, Nadine
Serfling, Robert
Kitte, Reni
Hilger, Nadja
Zhang, Chengkang
Gebhardt, Clara
Duenkel, Anna
Franz, Paul
Koehl, Ulrike
Fricke, Stephan
Tretbar, U. Sandy
author_facet von Auw, Nadine
Serfling, Robert
Kitte, Reni
Hilger, Nadja
Zhang, Chengkang
Gebhardt, Clara
Duenkel, Anna
Franz, Paul
Koehl, Ulrike
Fricke, Stephan
Tretbar, U. Sandy
author_sort von Auw, Nadine
collection PubMed
description Process development for transferring lab-scale research workflows to automated manufacturing procedures is critical for chimeric antigen receptor (CAR)-T cell therapies. Therefore, the key factor for cell viability, expansion, modification, and functionality is the optimal combination of medium and T cell activator as well as their regulatory compliance for later manufacturing under Good Manufacturing Practice (GMP). In this study, we compared two protocols for CAR-mRNA-modified T cell generation using our current lab-scale process, analyzed all mentioned parameters, and evaluated the protocols’ potential for upscaling and process development of mRNA-based CAR-T cell therapies.
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spelling pubmed-105980652023-10-26 Comparison of two lab-scale protocols for enhanced mRNA-based CAR-T cell generation and functionality von Auw, Nadine Serfling, Robert Kitte, Reni Hilger, Nadja Zhang, Chengkang Gebhardt, Clara Duenkel, Anna Franz, Paul Koehl, Ulrike Fricke, Stephan Tretbar, U. Sandy Sci Rep Article Process development for transferring lab-scale research workflows to automated manufacturing procedures is critical for chimeric antigen receptor (CAR)-T cell therapies. Therefore, the key factor for cell viability, expansion, modification, and functionality is the optimal combination of medium and T cell activator as well as their regulatory compliance for later manufacturing under Good Manufacturing Practice (GMP). In this study, we compared two protocols for CAR-mRNA-modified T cell generation using our current lab-scale process, analyzed all mentioned parameters, and evaluated the protocols’ potential for upscaling and process development of mRNA-based CAR-T cell therapies. Nature Publishing Group UK 2023-10-24 /pmc/articles/PMC10598065/ /pubmed/37875523 http://dx.doi.org/10.1038/s41598-023-45197-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
von Auw, Nadine
Serfling, Robert
Kitte, Reni
Hilger, Nadja
Zhang, Chengkang
Gebhardt, Clara
Duenkel, Anna
Franz, Paul
Koehl, Ulrike
Fricke, Stephan
Tretbar, U. Sandy
Comparison of two lab-scale protocols for enhanced mRNA-based CAR-T cell generation and functionality
title Comparison of two lab-scale protocols for enhanced mRNA-based CAR-T cell generation and functionality
title_full Comparison of two lab-scale protocols for enhanced mRNA-based CAR-T cell generation and functionality
title_fullStr Comparison of two lab-scale protocols for enhanced mRNA-based CAR-T cell generation and functionality
title_full_unstemmed Comparison of two lab-scale protocols for enhanced mRNA-based CAR-T cell generation and functionality
title_short Comparison of two lab-scale protocols for enhanced mRNA-based CAR-T cell generation and functionality
title_sort comparison of two lab-scale protocols for enhanced mrna-based car-t cell generation and functionality
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598065/
https://www.ncbi.nlm.nih.gov/pubmed/37875523
http://dx.doi.org/10.1038/s41598-023-45197-x
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