Cargando…
Simplified Preservation of Equivalent Pathways Spectroscopy
[Image: see text] Inspired by the recently proposed transverse mixing optimal control pulses (TROP) approach for improving signal in multidimensional magic-angle spinning (MAS) NMR experiments, we present simplified preservation of equivalent pathways spectroscopy (SPEPS). It transfers both transver...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598565/ https://www.ncbi.nlm.nih.gov/pubmed/37885577 http://dx.doi.org/10.1021/jacsau.3c00312 |
_version_ | 1785125582110982144 |
---|---|
author | Nimerovsky, Evgeny Varkey, Abel Cherian Kim, Myeongkyu Becker, Stefan Andreas, Loren B. |
author_facet | Nimerovsky, Evgeny Varkey, Abel Cherian Kim, Myeongkyu Becker, Stefan Andreas, Loren B. |
author_sort | Nimerovsky, Evgeny |
collection | PubMed |
description | [Image: see text] Inspired by the recently proposed transverse mixing optimal control pulses (TROP) approach for improving signal in multidimensional magic-angle spinning (MAS) NMR experiments, we present simplified preservation of equivalent pathways spectroscopy (SPEPS). It transfers both transverse components of magnetization that occur during indirect evolutions, theoretically enabling a √2 improvement in sensitivity for each such dimension. We compare SPEPS transfer with TROP and cross-polarization (CP) using membrane protein and fibril samples at MAS of 55 and 100 kHz. In three-dimensional (3D) (H)CANH spectra, SPEPS outperformed TROP and CP by factors of on average 1.16 and 1.69, respectively, for the membrane protein, but only a marginal improvement of 1.09 was observed for the fibril. These differences are discussed, making note of the longer transfer time used for CP, 14 ms, as compared with 2.9 and 3.6 ms for SPEPS and TROP, respectively. Using SPEPS for two transfers in the 3D (H)CANCO experiment resulted in an even larger benefit in signal intensity, with an average improvement of 1.82 as compared with CP. This results in multifold time savings, in particular considering the weaker peaks that are observed to benefit the most from SPEPS. |
format | Online Article Text |
id | pubmed-10598565 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-105985652023-10-26 Simplified Preservation of Equivalent Pathways Spectroscopy Nimerovsky, Evgeny Varkey, Abel Cherian Kim, Myeongkyu Becker, Stefan Andreas, Loren B. JACS Au [Image: see text] Inspired by the recently proposed transverse mixing optimal control pulses (TROP) approach for improving signal in multidimensional magic-angle spinning (MAS) NMR experiments, we present simplified preservation of equivalent pathways spectroscopy (SPEPS). It transfers both transverse components of magnetization that occur during indirect evolutions, theoretically enabling a √2 improvement in sensitivity for each such dimension. We compare SPEPS transfer with TROP and cross-polarization (CP) using membrane protein and fibril samples at MAS of 55 and 100 kHz. In three-dimensional (3D) (H)CANH spectra, SPEPS outperformed TROP and CP by factors of on average 1.16 and 1.69, respectively, for the membrane protein, but only a marginal improvement of 1.09 was observed for the fibril. These differences are discussed, making note of the longer transfer time used for CP, 14 ms, as compared with 2.9 and 3.6 ms for SPEPS and TROP, respectively. Using SPEPS for two transfers in the 3D (H)CANCO experiment resulted in an even larger benefit in signal intensity, with an average improvement of 1.82 as compared with CP. This results in multifold time savings, in particular considering the weaker peaks that are observed to benefit the most from SPEPS. American Chemical Society 2023-10-11 /pmc/articles/PMC10598565/ /pubmed/37885577 http://dx.doi.org/10.1021/jacsau.3c00312 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Nimerovsky, Evgeny Varkey, Abel Cherian Kim, Myeongkyu Becker, Stefan Andreas, Loren B. Simplified Preservation of Equivalent Pathways Spectroscopy |
title | Simplified Preservation
of Equivalent Pathways Spectroscopy |
title_full | Simplified Preservation
of Equivalent Pathways Spectroscopy |
title_fullStr | Simplified Preservation
of Equivalent Pathways Spectroscopy |
title_full_unstemmed | Simplified Preservation
of Equivalent Pathways Spectroscopy |
title_short | Simplified Preservation
of Equivalent Pathways Spectroscopy |
title_sort | simplified preservation
of equivalent pathways spectroscopy |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598565/ https://www.ncbi.nlm.nih.gov/pubmed/37885577 http://dx.doi.org/10.1021/jacsau.3c00312 |
work_keys_str_mv | AT nimerovskyevgeny simplifiedpreservationofequivalentpathwaysspectroscopy AT varkeyabelcherian simplifiedpreservationofequivalentpathwaysspectroscopy AT kimmyeongkyu simplifiedpreservationofequivalentpathwaysspectroscopy AT beckerstefan simplifiedpreservationofequivalentpathwaysspectroscopy AT andreaslorenb simplifiedpreservationofequivalentpathwaysspectroscopy |