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Mortui vivos docent: a modern revival of temporal bone plug harvests

Human temporal bones (HTBs) are invaluable resources for the study of otologic disorders and for evaluating novel treatment approaches. Given the high costs and technical expertise required to collect and process HTBs, there has been a decline in the number of otopathology laboratories. Our objectiv...

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Autores principales: Sagi, Varun, Kosaraju, Nikitha, Moore, Lindsay S., Mulders, Jip Y., Solyali, Mehmet, Ma, Xiaojie, Regula, Donald P., Hooper, Jody E., Stankovic, Konstantina M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598599/
https://www.ncbi.nlm.nih.gov/pubmed/37886674
http://dx.doi.org/10.3389/fnins.2023.1242831
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author Sagi, Varun
Kosaraju, Nikitha
Moore, Lindsay S.
Mulders, Jip Y.
Solyali, Mehmet
Ma, Xiaojie
Regula, Donald P.
Hooper, Jody E.
Stankovic, Konstantina M.
author_facet Sagi, Varun
Kosaraju, Nikitha
Moore, Lindsay S.
Mulders, Jip Y.
Solyali, Mehmet
Ma, Xiaojie
Regula, Donald P.
Hooper, Jody E.
Stankovic, Konstantina M.
author_sort Sagi, Varun
collection PubMed
description Human temporal bones (HTBs) are invaluable resources for the study of otologic disorders and for evaluating novel treatment approaches. Given the high costs and technical expertise required to collect and process HTBs, there has been a decline in the number of otopathology laboratories. Our objective is to encourage ongoing study of HTBs by outlining the necessary steps to establish a pipeline for collection and processing of HTBs. In this methods manuscript, we: (1) provide the design of a temporal bone plug sawblade that can be used to collect specimens from autopsy donors; (2) establish that decalcification time can be dramatically reduced from 9 to 3 months if ethylenediaminetetraacetic acid is combined with microwave tissue processing and periodic bone trimming; (3) show that serial sections of relatively-rapidly decalcified HTBs can be successfully immunostained for key inner ear proteins; (4) demonstrate how to drill down a HTB to the otic capsule within a few hours so that subsequent decalcification time can be further reduced to only weeks. We include photographs and videos to facilitate rapid dissemination of the developed methods. Collected HTBs can be used for many purposes, including, but not limited to device testing, imaging studies, education, histopathology, and molecular studies. As new technology develops, it is imperative to continue studying HTBs to further our understanding of the cellular and molecular underpinnings of otologic disorders.
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spelling pubmed-105985992023-10-26 Mortui vivos docent: a modern revival of temporal bone plug harvests Sagi, Varun Kosaraju, Nikitha Moore, Lindsay S. Mulders, Jip Y. Solyali, Mehmet Ma, Xiaojie Regula, Donald P. Hooper, Jody E. Stankovic, Konstantina M. Front Neurosci Neuroscience Human temporal bones (HTBs) are invaluable resources for the study of otologic disorders and for evaluating novel treatment approaches. Given the high costs and technical expertise required to collect and process HTBs, there has been a decline in the number of otopathology laboratories. Our objective is to encourage ongoing study of HTBs by outlining the necessary steps to establish a pipeline for collection and processing of HTBs. In this methods manuscript, we: (1) provide the design of a temporal bone plug sawblade that can be used to collect specimens from autopsy donors; (2) establish that decalcification time can be dramatically reduced from 9 to 3 months if ethylenediaminetetraacetic acid is combined with microwave tissue processing and periodic bone trimming; (3) show that serial sections of relatively-rapidly decalcified HTBs can be successfully immunostained for key inner ear proteins; (4) demonstrate how to drill down a HTB to the otic capsule within a few hours so that subsequent decalcification time can be further reduced to only weeks. We include photographs and videos to facilitate rapid dissemination of the developed methods. Collected HTBs can be used for many purposes, including, but not limited to device testing, imaging studies, education, histopathology, and molecular studies. As new technology develops, it is imperative to continue studying HTBs to further our understanding of the cellular and molecular underpinnings of otologic disorders. Frontiers Media S.A. 2023-10-11 /pmc/articles/PMC10598599/ /pubmed/37886674 http://dx.doi.org/10.3389/fnins.2023.1242831 Text en Copyright © 2023 Sagi, Kosaraju, Moore, Mulders, Solyali, Ma, Regula, Hooper and Stankovic. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Sagi, Varun
Kosaraju, Nikitha
Moore, Lindsay S.
Mulders, Jip Y.
Solyali, Mehmet
Ma, Xiaojie
Regula, Donald P.
Hooper, Jody E.
Stankovic, Konstantina M.
Mortui vivos docent: a modern revival of temporal bone plug harvests
title Mortui vivos docent: a modern revival of temporal bone plug harvests
title_full Mortui vivos docent: a modern revival of temporal bone plug harvests
title_fullStr Mortui vivos docent: a modern revival of temporal bone plug harvests
title_full_unstemmed Mortui vivos docent: a modern revival of temporal bone plug harvests
title_short Mortui vivos docent: a modern revival of temporal bone plug harvests
title_sort mortui vivos docent: a modern revival of temporal bone plug harvests
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598599/
https://www.ncbi.nlm.nih.gov/pubmed/37886674
http://dx.doi.org/10.3389/fnins.2023.1242831
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