Analyzing self-assembled spindle dynamics in fission yeast meiosis using in vivo fluorescence imaging

Chromosome segregation in female meiosis in many metazoans is mediated by acentrosomal spindles. The analysis of the dynamics of self-assembled spindles is a challenge due to the low availability of oocytes. Here, we present a protocol for analyzing self-assembled spindle dynamics in fission yeast m...

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Autores principales: Pineda-Santaella, Alberto, Martín-García, Rebeca, Fernández-Álvarez, Alfonso
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598709/
https://www.ncbi.nlm.nih.gov/pubmed/37864786
http://dx.doi.org/10.1016/j.xpro.2023.102655
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author Pineda-Santaella, Alberto
Martín-García, Rebeca
Fernández-Álvarez, Alfonso
author_facet Pineda-Santaella, Alberto
Martín-García, Rebeca
Fernández-Álvarez, Alfonso
author_sort Pineda-Santaella, Alberto
collection PubMed
description Chromosome segregation in female meiosis in many metazoans is mediated by acentrosomal spindles. The analysis of the dynamics of self-assembled spindles is a challenge due to the low availability of oocytes. Here, we present a protocol for analyzing self-assembled spindle dynamics in fission yeast meiosis using in vivo fluorescence imaging. We describe steps for starter culture preparation, meiosis induction, and sample preparation. We then detail procedures for acquisition and analysis of images of self-assembled spindles. For complete details on the use and execution of this protocol, please refer to Pineda-Santaella and Fernández-Álvarez (2019)(1) and Pineda-Santaella et al. (2021).(2)
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spelling pubmed-105987092023-10-26 Analyzing self-assembled spindle dynamics in fission yeast meiosis using in vivo fluorescence imaging Pineda-Santaella, Alberto Martín-García, Rebeca Fernández-Álvarez, Alfonso STAR Protoc Protocol Chromosome segregation in female meiosis in many metazoans is mediated by acentrosomal spindles. The analysis of the dynamics of self-assembled spindles is a challenge due to the low availability of oocytes. Here, we present a protocol for analyzing self-assembled spindle dynamics in fission yeast meiosis using in vivo fluorescence imaging. We describe steps for starter culture preparation, meiosis induction, and sample preparation. We then detail procedures for acquisition and analysis of images of self-assembled spindles. For complete details on the use and execution of this protocol, please refer to Pineda-Santaella and Fernández-Álvarez (2019)(1) and Pineda-Santaella et al. (2021).(2) Elsevier 2023-10-20 /pmc/articles/PMC10598709/ /pubmed/37864786 http://dx.doi.org/10.1016/j.xpro.2023.102655 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Pineda-Santaella, Alberto
Martín-García, Rebeca
Fernández-Álvarez, Alfonso
Analyzing self-assembled spindle dynamics in fission yeast meiosis using in vivo fluorescence imaging
title Analyzing self-assembled spindle dynamics in fission yeast meiosis using in vivo fluorescence imaging
title_full Analyzing self-assembled spindle dynamics in fission yeast meiosis using in vivo fluorescence imaging
title_fullStr Analyzing self-assembled spindle dynamics in fission yeast meiosis using in vivo fluorescence imaging
title_full_unstemmed Analyzing self-assembled spindle dynamics in fission yeast meiosis using in vivo fluorescence imaging
title_short Analyzing self-assembled spindle dynamics in fission yeast meiosis using in vivo fluorescence imaging
title_sort analyzing self-assembled spindle dynamics in fission yeast meiosis using in vivo fluorescence imaging
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598709/
https://www.ncbi.nlm.nih.gov/pubmed/37864786
http://dx.doi.org/10.1016/j.xpro.2023.102655
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AT fernandezalvarezalfonso analyzingselfassembledspindledynamicsinfissionyeastmeiosisusinginvivofluorescenceimaging

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