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Study on the metabolic process of phthalic acid driven proliferation of Rhizoctonia solani

INTRODUCTION: Continuous cropping obstacle seriously affects the quality and yield of Salvia miltiorrhiza, and the synergistic effect of root exudates and rhizosphere pathogenic microorganisms may be an important cause of continuous cropping obstacle. This study aimed to explore the effects of repre...

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Autores principales: Ju, Jidong, Zhou, Bingqian, Yang, Guohong, Fu, Xinyu, Wang, Xiao, Guo, Lanping, Liu, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598758/
https://www.ncbi.nlm.nih.gov/pubmed/37885668
http://dx.doi.org/10.3389/fpls.2023.1266916
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author Ju, Jidong
Zhou, Bingqian
Yang, Guohong
Fu, Xinyu
Wang, Xiao
Guo, Lanping
Liu, Wei
author_facet Ju, Jidong
Zhou, Bingqian
Yang, Guohong
Fu, Xinyu
Wang, Xiao
Guo, Lanping
Liu, Wei
author_sort Ju, Jidong
collection PubMed
description INTRODUCTION: Continuous cropping obstacle seriously affects the quality and yield of Salvia miltiorrhiza, and the synergistic effect of root exudates and rhizosphere pathogenic microorganisms may be an important cause of continuous cropping obstacle. This study aimed to explore the effects of representative organic acids on the growth and metabolism of specific microorganisms in the S. miltiorrhiza rhizosphere soil under continuous cropping, and clarify its mechanism. METHODS: The effect of phthalic acid (PA) on the growth and metabolism of Rhizoctonia solani was evaluated by mycelial growth inhibition method. Ultra-high performance liquid chromatography and tandem mass spectrometry were used to identify the differential metabolites of R. solani induced by exogenous PA. RESULTS: PA exerted a concentration-dependent effect on mycelial growth, biomass, intracellular polysaccharides con-tent, and total protein content in R. solani. A total of 1773 metabolites and 1040 differential metabolites were identified in the blank medium (CK), Fungi (CK + fungi), and PA-Fungi (CK + fungi + acid) groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the differential metabolites were mainly involved in the sugar, lipid, and protein metabolic pathways related to stable membrane structure and cell growth. DISCUSSION: The proliferation and metabolism network of R. solani induced by PA was proposed, and the enhancement of sugar, lipid, and amino acid metabolism was presumed to be related to the active resistance of cells to organic acid stress. These results offer new in-sights into the effects of PA metabolism on promoting R. solani proliferation, and provide theoretical support for further optimizing the rhizosphere microecological environment of Salvia miltiorrhiza continuous cropping soil and reducing continuous cropping obstacle.
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spelling pubmed-105987582023-10-26 Study on the metabolic process of phthalic acid driven proliferation of Rhizoctonia solani Ju, Jidong Zhou, Bingqian Yang, Guohong Fu, Xinyu Wang, Xiao Guo, Lanping Liu, Wei Front Plant Sci Plant Science INTRODUCTION: Continuous cropping obstacle seriously affects the quality and yield of Salvia miltiorrhiza, and the synergistic effect of root exudates and rhizosphere pathogenic microorganisms may be an important cause of continuous cropping obstacle. This study aimed to explore the effects of representative organic acids on the growth and metabolism of specific microorganisms in the S. miltiorrhiza rhizosphere soil under continuous cropping, and clarify its mechanism. METHODS: The effect of phthalic acid (PA) on the growth and metabolism of Rhizoctonia solani was evaluated by mycelial growth inhibition method. Ultra-high performance liquid chromatography and tandem mass spectrometry were used to identify the differential metabolites of R. solani induced by exogenous PA. RESULTS: PA exerted a concentration-dependent effect on mycelial growth, biomass, intracellular polysaccharides con-tent, and total protein content in R. solani. A total of 1773 metabolites and 1040 differential metabolites were identified in the blank medium (CK), Fungi (CK + fungi), and PA-Fungi (CK + fungi + acid) groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the differential metabolites were mainly involved in the sugar, lipid, and protein metabolic pathways related to stable membrane structure and cell growth. DISCUSSION: The proliferation and metabolism network of R. solani induced by PA was proposed, and the enhancement of sugar, lipid, and amino acid metabolism was presumed to be related to the active resistance of cells to organic acid stress. These results offer new in-sights into the effects of PA metabolism on promoting R. solani proliferation, and provide theoretical support for further optimizing the rhizosphere microecological environment of Salvia miltiorrhiza continuous cropping soil and reducing continuous cropping obstacle. Frontiers Media S.A. 2023-10-11 /pmc/articles/PMC10598758/ /pubmed/37885668 http://dx.doi.org/10.3389/fpls.2023.1266916 Text en Copyright © 2023 Ju, Zhou, Yang, Fu, Wang, Guo and Liu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ju, Jidong
Zhou, Bingqian
Yang, Guohong
Fu, Xinyu
Wang, Xiao
Guo, Lanping
Liu, Wei
Study on the metabolic process of phthalic acid driven proliferation of Rhizoctonia solani
title Study on the metabolic process of phthalic acid driven proliferation of Rhizoctonia solani
title_full Study on the metabolic process of phthalic acid driven proliferation of Rhizoctonia solani
title_fullStr Study on the metabolic process of phthalic acid driven proliferation of Rhizoctonia solani
title_full_unstemmed Study on the metabolic process of phthalic acid driven proliferation of Rhizoctonia solani
title_short Study on the metabolic process of phthalic acid driven proliferation of Rhizoctonia solani
title_sort study on the metabolic process of phthalic acid driven proliferation of rhizoctonia solani
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598758/
https://www.ncbi.nlm.nih.gov/pubmed/37885668
http://dx.doi.org/10.3389/fpls.2023.1266916
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