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FlbZIP12 gene enhances drought tolerance via modulating flavonoid biosynthesis in Fagopyrum leptopodum

Karst lands provide a poor substrate to support plant growth, as they are low in nutrients and water content. Common buckwheat (Fagopyrum esculentum) is becoming a popular crop for its gluten-free grains and their high levels of phenolic compounds, but buckwheat yields are affected by high water req...

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Autores principales: Wang, Anhu, Liu, Yu, Li, Qiujie, Li, Xiaoyi, Zhang, Xinrong, Kong, Jiao, Liu, Zhibing, Yang, Yi, Wang, Jianmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598875/
https://www.ncbi.nlm.nih.gov/pubmed/37885669
http://dx.doi.org/10.3389/fpls.2023.1279468
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author Wang, Anhu
Liu, Yu
Li, Qiujie
Li, Xiaoyi
Zhang, Xinrong
Kong, Jiao
Liu, Zhibing
Yang, Yi
Wang, Jianmei
author_facet Wang, Anhu
Liu, Yu
Li, Qiujie
Li, Xiaoyi
Zhang, Xinrong
Kong, Jiao
Liu, Zhibing
Yang, Yi
Wang, Jianmei
author_sort Wang, Anhu
collection PubMed
description Karst lands provide a poor substrate to support plant growth, as they are low in nutrients and water content. Common buckwheat (Fagopyrum esculentum) is becoming a popular crop for its gluten-free grains and their high levels of phenolic compounds, but buckwheat yields are affected by high water requirements during grain filling. Here, we describe a wild population of drought-tolerant Fagopyrum leptopodum and its potential for enhancing drought tolerance in cultivated buckwheat. We determined that the expression of a gene encoding a Basic leucine zipper (bZIP) transcription factor, FlbZIP12, from F. leptopodum is induced by abiotic stresses, including treatment with the phytohormone abscisic acid, salt, and polyethylene glycol. In addition, we show that overexpressing FlbZIP12 in Tartary buckwheat (Fagopyrum tataricum) root hairs promoted drought tolerance by increasing the activities of the enzymes superoxide dismutase and catalase, decreasing malondialdehyde content, and upregulating the expression of stress-related genes. Notably, FlbZIP12 overexpression induced the expression of key genes involved in flavonoid biosynthesis. We also determined that FlbZIP12 interacts with protein kinases from the FlSnRK2 family in vitro and in vivo. Taken together, our results provide a theoretical basis for improving drought tolerance in buckwheat via modulating the expression of FlbZIP12 and flavonoid contents.
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spelling pubmed-105988752023-10-26 FlbZIP12 gene enhances drought tolerance via modulating flavonoid biosynthesis in Fagopyrum leptopodum Wang, Anhu Liu, Yu Li, Qiujie Li, Xiaoyi Zhang, Xinrong Kong, Jiao Liu, Zhibing Yang, Yi Wang, Jianmei Front Plant Sci Plant Science Karst lands provide a poor substrate to support plant growth, as they are low in nutrients and water content. Common buckwheat (Fagopyrum esculentum) is becoming a popular crop for its gluten-free grains and their high levels of phenolic compounds, but buckwheat yields are affected by high water requirements during grain filling. Here, we describe a wild population of drought-tolerant Fagopyrum leptopodum and its potential for enhancing drought tolerance in cultivated buckwheat. We determined that the expression of a gene encoding a Basic leucine zipper (bZIP) transcription factor, FlbZIP12, from F. leptopodum is induced by abiotic stresses, including treatment with the phytohormone abscisic acid, salt, and polyethylene glycol. In addition, we show that overexpressing FlbZIP12 in Tartary buckwheat (Fagopyrum tataricum) root hairs promoted drought tolerance by increasing the activities of the enzymes superoxide dismutase and catalase, decreasing malondialdehyde content, and upregulating the expression of stress-related genes. Notably, FlbZIP12 overexpression induced the expression of key genes involved in flavonoid biosynthesis. We also determined that FlbZIP12 interacts with protein kinases from the FlSnRK2 family in vitro and in vivo. Taken together, our results provide a theoretical basis for improving drought tolerance in buckwheat via modulating the expression of FlbZIP12 and flavonoid contents. Frontiers Media S.A. 2023-10-11 /pmc/articles/PMC10598875/ /pubmed/37885669 http://dx.doi.org/10.3389/fpls.2023.1279468 Text en Copyright © 2023 Wang, Liu, Li, Li, Zhang, Kong, Liu, Yang and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wang, Anhu
Liu, Yu
Li, Qiujie
Li, Xiaoyi
Zhang, Xinrong
Kong, Jiao
Liu, Zhibing
Yang, Yi
Wang, Jianmei
FlbZIP12 gene enhances drought tolerance via modulating flavonoid biosynthesis in Fagopyrum leptopodum
title FlbZIP12 gene enhances drought tolerance via modulating flavonoid biosynthesis in Fagopyrum leptopodum
title_full FlbZIP12 gene enhances drought tolerance via modulating flavonoid biosynthesis in Fagopyrum leptopodum
title_fullStr FlbZIP12 gene enhances drought tolerance via modulating flavonoid biosynthesis in Fagopyrum leptopodum
title_full_unstemmed FlbZIP12 gene enhances drought tolerance via modulating flavonoid biosynthesis in Fagopyrum leptopodum
title_short FlbZIP12 gene enhances drought tolerance via modulating flavonoid biosynthesis in Fagopyrum leptopodum
title_sort flbzip12 gene enhances drought tolerance via modulating flavonoid biosynthesis in fagopyrum leptopodum
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598875/
https://www.ncbi.nlm.nih.gov/pubmed/37885669
http://dx.doi.org/10.3389/fpls.2023.1279468
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