Cargando…

Association between Yili goose sperm motility and expression profiles of mRNA and miRNA in testis

BACKGROUND: The study was conducted to find out the candidate microRNA (miRNA) and genes that associated with sperm motility of Yili goose through small RNA sequencing of testicular tissue of Yili goose, and provide a theoretical basis for the study of the regulation mechanism of sperm motility of Y...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Yingping, Zhang, Lihua, Li, Haiying, Zhao, Xiaoyu, Ding, Yawen, Yao, Yingying, Wang, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599010/
https://www.ncbi.nlm.nih.gov/pubmed/37875805
http://dx.doi.org/10.1186/s12864-023-09727-1
_version_ 1785125683032227840
author Wu, Yingping
Zhang, Lihua
Li, Haiying
Zhao, Xiaoyu
Ding, Yawen
Yao, Yingying
Wang, Ling
author_facet Wu, Yingping
Zhang, Lihua
Li, Haiying
Zhao, Xiaoyu
Ding, Yawen
Yao, Yingying
Wang, Ling
author_sort Wu, Yingping
collection PubMed
description BACKGROUND: The study was conducted to find out the candidate microRNA (miRNA) and genes that associated with sperm motility of Yili goose through small RNA sequencing of testicular tissue of Yili goose, and provide a theoretical basis for the study of the regulation mechanism of sperm motility of Yili goose gander. RESULTS: In this study, five male geese with high sperm motility and five male geese with low sperm motility were slaughtered to obtain their testis tissues for small RNA sequencing, and biological information methods were used for data analysis. The results showed that a total of 1575 known miRNAs and 68 novel miRNAs were identified in the testis tissue of Yili goose, and 71 differentially expressed miRNAs and 660 differentially expressed genes were screened. GO functional analysis showed that miRNAs target genes were mainly involved in the binding, kinase activity, structural constituent of cytoskeleton and intermediate filament cytoskeleton. KEGG functional analysis showed that miRNAs target genes were significantly enriched in arginine and proline metabolism, glycolysis / gluconeogenesis, fructose and mannose metabolism and beta-Alanine metabolism and other pathways. miRNAs-mRNAs interaction network suggests miR-140/miR-140-3p/miR-140-3p-NKAIN3, let-7d-BTG1 and miR-145-5p/miR -145a-5p-CLEC2E may play an important role in testis development and spermatogenesis. CONCLUSIONS: The results of this study suggest that the sperm motility of Yili goose may be regulated by different miRNAs, and the target genes are significantly enriched in pathways related to sperm metabolism, indicating that miRNAs affect the sperm motility of Yili goose by regulating the metabolic process of sperm and the expression of related genes. This study can provide a reference for revealing the regulation mechanism of Yili goose sperm motility at the molecular level. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09727-1.
format Online
Article
Text
id pubmed-10599010
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-105990102023-10-26 Association between Yili goose sperm motility and expression profiles of mRNA and miRNA in testis Wu, Yingping Zhang, Lihua Li, Haiying Zhao, Xiaoyu Ding, Yawen Yao, Yingying Wang, Ling BMC Genomics Research BACKGROUND: The study was conducted to find out the candidate microRNA (miRNA) and genes that associated with sperm motility of Yili goose through small RNA sequencing of testicular tissue of Yili goose, and provide a theoretical basis for the study of the regulation mechanism of sperm motility of Yili goose gander. RESULTS: In this study, five male geese with high sperm motility and five male geese with low sperm motility were slaughtered to obtain their testis tissues for small RNA sequencing, and biological information methods were used for data analysis. The results showed that a total of 1575 known miRNAs and 68 novel miRNAs were identified in the testis tissue of Yili goose, and 71 differentially expressed miRNAs and 660 differentially expressed genes were screened. GO functional analysis showed that miRNAs target genes were mainly involved in the binding, kinase activity, structural constituent of cytoskeleton and intermediate filament cytoskeleton. KEGG functional analysis showed that miRNAs target genes were significantly enriched in arginine and proline metabolism, glycolysis / gluconeogenesis, fructose and mannose metabolism and beta-Alanine metabolism and other pathways. miRNAs-mRNAs interaction network suggests miR-140/miR-140-3p/miR-140-3p-NKAIN3, let-7d-BTG1 and miR-145-5p/miR -145a-5p-CLEC2E may play an important role in testis development and spermatogenesis. CONCLUSIONS: The results of this study suggest that the sperm motility of Yili goose may be regulated by different miRNAs, and the target genes are significantly enriched in pathways related to sperm metabolism, indicating that miRNAs affect the sperm motility of Yili goose by regulating the metabolic process of sperm and the expression of related genes. This study can provide a reference for revealing the regulation mechanism of Yili goose sperm motility at the molecular level. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09727-1. BioMed Central 2023-10-24 /pmc/articles/PMC10599010/ /pubmed/37875805 http://dx.doi.org/10.1186/s12864-023-09727-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wu, Yingping
Zhang, Lihua
Li, Haiying
Zhao, Xiaoyu
Ding, Yawen
Yao, Yingying
Wang, Ling
Association between Yili goose sperm motility and expression profiles of mRNA and miRNA in testis
title Association between Yili goose sperm motility and expression profiles of mRNA and miRNA in testis
title_full Association between Yili goose sperm motility and expression profiles of mRNA and miRNA in testis
title_fullStr Association between Yili goose sperm motility and expression profiles of mRNA and miRNA in testis
title_full_unstemmed Association between Yili goose sperm motility and expression profiles of mRNA and miRNA in testis
title_short Association between Yili goose sperm motility and expression profiles of mRNA and miRNA in testis
title_sort association between yili goose sperm motility and expression profiles of mrna and mirna in testis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599010/
https://www.ncbi.nlm.nih.gov/pubmed/37875805
http://dx.doi.org/10.1186/s12864-023-09727-1
work_keys_str_mv AT wuyingping associationbetweenyiligoosespermmotilityandexpressionprofilesofmrnaandmirnaintestis
AT zhanglihua associationbetweenyiligoosespermmotilityandexpressionprofilesofmrnaandmirnaintestis
AT lihaiying associationbetweenyiligoosespermmotilityandexpressionprofilesofmrnaandmirnaintestis
AT zhaoxiaoyu associationbetweenyiligoosespermmotilityandexpressionprofilesofmrnaandmirnaintestis
AT dingyawen associationbetweenyiligoosespermmotilityandexpressionprofilesofmrnaandmirnaintestis
AT yaoyingying associationbetweenyiligoosespermmotilityandexpressionprofilesofmrnaandmirnaintestis
AT wangling associationbetweenyiligoosespermmotilityandexpressionprofilesofmrnaandmirnaintestis