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A novel Galleria mellonella experimental model for zoonotic pathogen Brucella

Brucellosis is a major threat to public health and animal husbandry. Several in vivo vertebrate models, such as mice, guinea pigs, and nonhuman primates, have been used to study Brucella pathogenesis, bacteria-host interactions, and vaccine efficacy. However, these models have limitations whereas th...

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Autores principales: Wang, Shuyi, Yin, Ying, Zai, Xiaodong, Gu, Yanfei, Guo, Fengyu, Shao, Fangze, Zhang, Yue, Li, Yaohui, Li, Ruihua, Zhang, Jun, Xu, Junjie, Chen, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599192/
https://www.ncbi.nlm.nih.gov/pubmed/37817444
http://dx.doi.org/10.1080/21505594.2023.2268496
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author Wang, Shuyi
Yin, Ying
Zai, Xiaodong
Gu, Yanfei
Guo, Fengyu
Shao, Fangze
Zhang, Yue
Li, Yaohui
Li, Ruihua
Zhang, Jun
Xu, Junjie
Chen, Wei
author_facet Wang, Shuyi
Yin, Ying
Zai, Xiaodong
Gu, Yanfei
Guo, Fengyu
Shao, Fangze
Zhang, Yue
Li, Yaohui
Li, Ruihua
Zhang, Jun
Xu, Junjie
Chen, Wei
author_sort Wang, Shuyi
collection PubMed
description Brucellosis is a major threat to public health and animal husbandry. Several in vivo vertebrate models, such as mice, guinea pigs, and nonhuman primates, have been used to study Brucella pathogenesis, bacteria-host interactions, and vaccine efficacy. However, these models have limitations whereas the invertebrate Galleria mellonella model is a cost-effective and ethical alternative. The aim of the present study was to examine the invertebrate G. mellonella as an in vivo infection model for Brucella. Infection assays were employed to validate the fitness of the larval model for Brucella infection and virulence evaluation. The protective efficacy of immune sera was evaluated by pre-incubated with a lethal dose of bacteria before infection. The consistency between the mouse model and the larval model was confirmed by assessing the protective efficacy of two Brucella vaccine strains. The results show that G. mellonella could be infected by Brucella strains, in a dose- and temperature-dependent way. Moreover, this larval model can effectively evaluate the virulence of Brucella strains in a manner consistent with that of mammalian infection models. Importantly, this model can assess the protective efficacy of vaccine immune sera within a day. Further investigation implied that haemolymph played a crucial role in the protective efficacy of immune sera. In conclusion, G. mellonella could serve as a quick, efficient, and reliable model for evaluating the virulence of Brucella strains and efficacy of immune sera in an ethical manner.
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spelling pubmed-105991922023-10-26 A novel Galleria mellonella experimental model for zoonotic pathogen Brucella Wang, Shuyi Yin, Ying Zai, Xiaodong Gu, Yanfei Guo, Fengyu Shao, Fangze Zhang, Yue Li, Yaohui Li, Ruihua Zhang, Jun Xu, Junjie Chen, Wei Virulence Research Article Brucellosis is a major threat to public health and animal husbandry. Several in vivo vertebrate models, such as mice, guinea pigs, and nonhuman primates, have been used to study Brucella pathogenesis, bacteria-host interactions, and vaccine efficacy. However, these models have limitations whereas the invertebrate Galleria mellonella model is a cost-effective and ethical alternative. The aim of the present study was to examine the invertebrate G. mellonella as an in vivo infection model for Brucella. Infection assays were employed to validate the fitness of the larval model for Brucella infection and virulence evaluation. The protective efficacy of immune sera was evaluated by pre-incubated with a lethal dose of bacteria before infection. The consistency between the mouse model and the larval model was confirmed by assessing the protective efficacy of two Brucella vaccine strains. The results show that G. mellonella could be infected by Brucella strains, in a dose- and temperature-dependent way. Moreover, this larval model can effectively evaluate the virulence of Brucella strains in a manner consistent with that of mammalian infection models. Importantly, this model can assess the protective efficacy of vaccine immune sera within a day. Further investigation implied that haemolymph played a crucial role in the protective efficacy of immune sera. In conclusion, G. mellonella could serve as a quick, efficient, and reliable model for evaluating the virulence of Brucella strains and efficacy of immune sera in an ethical manner. Taylor & Francis 2023-10-10 /pmc/articles/PMC10599192/ /pubmed/37817444 http://dx.doi.org/10.1080/21505594.2023.2268496 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.
spellingShingle Research Article
Wang, Shuyi
Yin, Ying
Zai, Xiaodong
Gu, Yanfei
Guo, Fengyu
Shao, Fangze
Zhang, Yue
Li, Yaohui
Li, Ruihua
Zhang, Jun
Xu, Junjie
Chen, Wei
A novel Galleria mellonella experimental model for zoonotic pathogen Brucella
title A novel Galleria mellonella experimental model for zoonotic pathogen Brucella
title_full A novel Galleria mellonella experimental model for zoonotic pathogen Brucella
title_fullStr A novel Galleria mellonella experimental model for zoonotic pathogen Brucella
title_full_unstemmed A novel Galleria mellonella experimental model for zoonotic pathogen Brucella
title_short A novel Galleria mellonella experimental model for zoonotic pathogen Brucella
title_sort novel galleria mellonella experimental model for zoonotic pathogen brucella
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599192/
https://www.ncbi.nlm.nih.gov/pubmed/37817444
http://dx.doi.org/10.1080/21505594.2023.2268496
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