Cargando…

Three-Dimensional Printed Hydroxyapatite Bone Substitutes Designed by a Novel Periodic Minimal Surface Algorithm Are Highly Osteoconductive

Autologous bone remains the gold standard bone substitute in clinical practice. Therefore, the microarchitecture of newly developed synthetic bone substitutes, which reflects the spatial distribution of materials in the scaffold, aims to recapitulate the natural bone microarchitecture. However, the...

Descripción completa

Detalles Bibliográficos
Autores principales: Maevskaia, Ekaterina, Khera, Nupur, Ghayor, Chafik, Bhattacharya, Indranil, Guerrero, Julien, Nicholls, Flora, Waldvogel, Christian, Bärtschi, Ralph, Fritschi, Lea, Salamon, Dániel, Özcan, Mutlu, Malgaroli, Patrick, Seiler, Daniel, de Wild, Michael, Weber, Franz E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc., publishers 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599419/
https://www.ncbi.nlm.nih.gov/pubmed/37886403
http://dx.doi.org/10.1089/3dp.2022.0134
_version_ 1785125765345443840
author Maevskaia, Ekaterina
Khera, Nupur
Ghayor, Chafik
Bhattacharya, Indranil
Guerrero, Julien
Nicholls, Flora
Waldvogel, Christian
Bärtschi, Ralph
Fritschi, Lea
Salamon, Dániel
Özcan, Mutlu
Malgaroli, Patrick
Seiler, Daniel
de Wild, Michael
Weber, Franz E.
author_facet Maevskaia, Ekaterina
Khera, Nupur
Ghayor, Chafik
Bhattacharya, Indranil
Guerrero, Julien
Nicholls, Flora
Waldvogel, Christian
Bärtschi, Ralph
Fritschi, Lea
Salamon, Dániel
Özcan, Mutlu
Malgaroli, Patrick
Seiler, Daniel
de Wild, Michael
Weber, Franz E.
author_sort Maevskaia, Ekaterina
collection PubMed
description Autologous bone remains the gold standard bone substitute in clinical practice. Therefore, the microarchitecture of newly developed synthetic bone substitutes, which reflects the spatial distribution of materials in the scaffold, aims to recapitulate the natural bone microarchitecture. However, the natural bone microarchitecture is optimized to obtain a mechanically stable, lightweight structure adapted to the biomechanical loading situation. In the context of synthetic bone substitutes, the application of a Triply Periodic Minimum Surface (TPMS) algorithm can yield stable lightweight microarchitectures that, despite their demanding architectural complexity, can be produced by additive manufacturing. In this study, we applied the TPMS derivative Adaptive Density Minimal Surfaces (ADMS) algorithm to produce scaffolds from hydroxyapatite (HA) using a lithography-based layer-by-layer methodology and compared them with an established highly osteoconductive lattice microarchitecture. We characterized them for compression strength, osteoconductivity, and bone regeneration. The in vivo results, based on a rabbit calvaria defect model, showed that bony ingrowth into ADMS constructs as a measure of osteoconduction depended on minimal constriction as it limited the maximum apparent pore diameter in these scaffolds to 1.53 mm. Osteoconduction decreased significantly at a diameter of 1.76 mm. The most suitable ADMS microarchitecture was as osteoconductive as a highly osteoconductive orthogonal lattice microarchitecture in noncritical- and critical-size calvarial defects. However, the compression strength and microarchitectural integrity in vivo were significantly higher for scaffolds with their microarchitecture based on the ADMS algorithm when compared with high-connectivity lattice microarchitectures. Therefore, bone substitutes with high osteoconductivity can be designed with the advantages of the ADMS-based microarchitectures. As TPMS and ADMS microarchitectures are true lightweight structures optimized for high mechanical stability with a minimal amount of material, such microarchitectures appear most suitable for bone substitutes used in clinical settings to treat bone defects in weight-bearing and non-weight-bearing sites.
format Online
Article
Text
id pubmed-10599419
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Mary Ann Liebert, Inc., publishers
record_format MEDLINE/PubMed
spelling pubmed-105994192023-10-26 Three-Dimensional Printed Hydroxyapatite Bone Substitutes Designed by a Novel Periodic Minimal Surface Algorithm Are Highly Osteoconductive Maevskaia, Ekaterina Khera, Nupur Ghayor, Chafik Bhattacharya, Indranil Guerrero, Julien Nicholls, Flora Waldvogel, Christian Bärtschi, Ralph Fritschi, Lea Salamon, Dániel Özcan, Mutlu Malgaroli, Patrick Seiler, Daniel de Wild, Michael Weber, Franz E. 3D Print Addit Manuf Original Articles Autologous bone remains the gold standard bone substitute in clinical practice. Therefore, the microarchitecture of newly developed synthetic bone substitutes, which reflects the spatial distribution of materials in the scaffold, aims to recapitulate the natural bone microarchitecture. However, the natural bone microarchitecture is optimized to obtain a mechanically stable, lightweight structure adapted to the biomechanical loading situation. In the context of synthetic bone substitutes, the application of a Triply Periodic Minimum Surface (TPMS) algorithm can yield stable lightweight microarchitectures that, despite their demanding architectural complexity, can be produced by additive manufacturing. In this study, we applied the TPMS derivative Adaptive Density Minimal Surfaces (ADMS) algorithm to produce scaffolds from hydroxyapatite (HA) using a lithography-based layer-by-layer methodology and compared them with an established highly osteoconductive lattice microarchitecture. We characterized them for compression strength, osteoconductivity, and bone regeneration. The in vivo results, based on a rabbit calvaria defect model, showed that bony ingrowth into ADMS constructs as a measure of osteoconduction depended on minimal constriction as it limited the maximum apparent pore diameter in these scaffolds to 1.53 mm. Osteoconduction decreased significantly at a diameter of 1.76 mm. The most suitable ADMS microarchitecture was as osteoconductive as a highly osteoconductive orthogonal lattice microarchitecture in noncritical- and critical-size calvarial defects. However, the compression strength and microarchitectural integrity in vivo were significantly higher for scaffolds with their microarchitecture based on the ADMS algorithm when compared with high-connectivity lattice microarchitectures. Therefore, bone substitutes with high osteoconductivity can be designed with the advantages of the ADMS-based microarchitectures. As TPMS and ADMS microarchitectures are true lightweight structures optimized for high mechanical stability with a minimal amount of material, such microarchitectures appear most suitable for bone substitutes used in clinical settings to treat bone defects in weight-bearing and non-weight-bearing sites. Mary Ann Liebert, Inc., publishers 2023-10-01 2023-10-10 /pmc/articles/PMC10599419/ /pubmed/37886403 http://dx.doi.org/10.1089/3dp.2022.0134 Text en © Ekaterina Maevskaia et al., 2023; Published by Mary Ann Liebert, Inc. https://creativecommons.org/licenses/by/4.0/This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Maevskaia, Ekaterina
Khera, Nupur
Ghayor, Chafik
Bhattacharya, Indranil
Guerrero, Julien
Nicholls, Flora
Waldvogel, Christian
Bärtschi, Ralph
Fritschi, Lea
Salamon, Dániel
Özcan, Mutlu
Malgaroli, Patrick
Seiler, Daniel
de Wild, Michael
Weber, Franz E.
Three-Dimensional Printed Hydroxyapatite Bone Substitutes Designed by a Novel Periodic Minimal Surface Algorithm Are Highly Osteoconductive
title Three-Dimensional Printed Hydroxyapatite Bone Substitutes Designed by a Novel Periodic Minimal Surface Algorithm Are Highly Osteoconductive
title_full Three-Dimensional Printed Hydroxyapatite Bone Substitutes Designed by a Novel Periodic Minimal Surface Algorithm Are Highly Osteoconductive
title_fullStr Three-Dimensional Printed Hydroxyapatite Bone Substitutes Designed by a Novel Periodic Minimal Surface Algorithm Are Highly Osteoconductive
title_full_unstemmed Three-Dimensional Printed Hydroxyapatite Bone Substitutes Designed by a Novel Periodic Minimal Surface Algorithm Are Highly Osteoconductive
title_short Three-Dimensional Printed Hydroxyapatite Bone Substitutes Designed by a Novel Periodic Minimal Surface Algorithm Are Highly Osteoconductive
title_sort three-dimensional printed hydroxyapatite bone substitutes designed by a novel periodic minimal surface algorithm are highly osteoconductive
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599419/
https://www.ncbi.nlm.nih.gov/pubmed/37886403
http://dx.doi.org/10.1089/3dp.2022.0134
work_keys_str_mv AT maevskaiaekaterina threedimensionalprintedhydroxyapatitebonesubstitutesdesignedbyanovelperiodicminimalsurfacealgorithmarehighlyosteoconductive
AT kheranupur threedimensionalprintedhydroxyapatitebonesubstitutesdesignedbyanovelperiodicminimalsurfacealgorithmarehighlyosteoconductive
AT ghayorchafik threedimensionalprintedhydroxyapatitebonesubstitutesdesignedbyanovelperiodicminimalsurfacealgorithmarehighlyosteoconductive
AT bhattacharyaindranil threedimensionalprintedhydroxyapatitebonesubstitutesdesignedbyanovelperiodicminimalsurfacealgorithmarehighlyosteoconductive
AT guerrerojulien threedimensionalprintedhydroxyapatitebonesubstitutesdesignedbyanovelperiodicminimalsurfacealgorithmarehighlyosteoconductive
AT nichollsflora threedimensionalprintedhydroxyapatitebonesubstitutesdesignedbyanovelperiodicminimalsurfacealgorithmarehighlyosteoconductive
AT waldvogelchristian threedimensionalprintedhydroxyapatitebonesubstitutesdesignedbyanovelperiodicminimalsurfacealgorithmarehighlyosteoconductive
AT bartschiralph threedimensionalprintedhydroxyapatitebonesubstitutesdesignedbyanovelperiodicminimalsurfacealgorithmarehighlyosteoconductive
AT fritschilea threedimensionalprintedhydroxyapatitebonesubstitutesdesignedbyanovelperiodicminimalsurfacealgorithmarehighlyosteoconductive
AT salamondaniel threedimensionalprintedhydroxyapatitebonesubstitutesdesignedbyanovelperiodicminimalsurfacealgorithmarehighlyosteoconductive
AT ozcanmutlu threedimensionalprintedhydroxyapatitebonesubstitutesdesignedbyanovelperiodicminimalsurfacealgorithmarehighlyosteoconductive
AT malgarolipatrick threedimensionalprintedhydroxyapatitebonesubstitutesdesignedbyanovelperiodicminimalsurfacealgorithmarehighlyosteoconductive
AT seilerdaniel threedimensionalprintedhydroxyapatitebonesubstitutesdesignedbyanovelperiodicminimalsurfacealgorithmarehighlyosteoconductive
AT dewildmichael threedimensionalprintedhydroxyapatitebonesubstitutesdesignedbyanovelperiodicminimalsurfacealgorithmarehighlyosteoconductive
AT weberfranze threedimensionalprintedhydroxyapatitebonesubstitutesdesignedbyanovelperiodicminimalsurfacealgorithmarehighlyosteoconductive