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Effect of ascorbic acid on cellular respiration with mitochondrial reductase in gingival fibroblast

Vitamin C or L-ascorbic acid has diverse functions in the body, especially healing promotion in tissue injury via participating in the hydroxylation reactions required for collagen formation. Systemic administration of vitamin C plays an important role on gingival fibroblast proliferation and functi...

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Detalles Bibliográficos
Autores principales: Katariya, Chanchal, Jayakumar, ND, Kumar, Raghunandha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Biomedical Informatics 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599683/
https://www.ncbi.nlm.nih.gov/pubmed/37886148
http://dx.doi.org/10.6026/97320630019552
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author Katariya, Chanchal
Jayakumar, ND
Kumar, Raghunandha
author_facet Katariya, Chanchal
Jayakumar, ND
Kumar, Raghunandha
author_sort Katariya, Chanchal
collection PubMed
description Vitamin C or L-ascorbic acid has diverse functions in the body, especially healing promotion in tissue injury via participating in the hydroxylation reactions required for collagen formation. Systemic administration of vitamin C plays an important role on gingival fibroblast proliferation and functions. Whether local or rinsing administration of vitamin C alters gingival fibroblast wound healing behavior remains unclear. Therefore, it is of interest to investigate the effect of vitamin C on gingival fibroblast behavior utilizing cell culture. Primary human gingival fibroblasts isolated from gingival tissue were rinsed with medium containing various concentrations of vitamin C. Cell migration, cell viability was assessed using MTT assay. The viability assay showed >95% live cells, and no significant (P > 0.05) difference in these values was observed at different concentrations at 24 hrs. The levels of cell proliferation were not significantly different among the control and experimental groups in 24 hrs experimental time-points (p > 0.05). Vitamin C is nontoxic and can be used for further experiments to validate for clinical application. This was further confirmed with morphological examination after 24hrs incubation on control and experimental group drugs observed under the phase contrast microscope.
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spelling pubmed-105996832023-10-26 Effect of ascorbic acid on cellular respiration with mitochondrial reductase in gingival fibroblast Katariya, Chanchal Jayakumar, ND Kumar, Raghunandha Bioinformation Research Article Vitamin C or L-ascorbic acid has diverse functions in the body, especially healing promotion in tissue injury via participating in the hydroxylation reactions required for collagen formation. Systemic administration of vitamin C plays an important role on gingival fibroblast proliferation and functions. Whether local or rinsing administration of vitamin C alters gingival fibroblast wound healing behavior remains unclear. Therefore, it is of interest to investigate the effect of vitamin C on gingival fibroblast behavior utilizing cell culture. Primary human gingival fibroblasts isolated from gingival tissue were rinsed with medium containing various concentrations of vitamin C. Cell migration, cell viability was assessed using MTT assay. The viability assay showed >95% live cells, and no significant (P > 0.05) difference in these values was observed at different concentrations at 24 hrs. The levels of cell proliferation were not significantly different among the control and experimental groups in 24 hrs experimental time-points (p > 0.05). Vitamin C is nontoxic and can be used for further experiments to validate for clinical application. This was further confirmed with morphological examination after 24hrs incubation on control and experimental group drugs observed under the phase contrast microscope. Biomedical Informatics 2023-05-31 /pmc/articles/PMC10599683/ /pubmed/37886148 http://dx.doi.org/10.6026/97320630019552 Text en © 2023 Biomedical Informatics https://creativecommons.org/licenses/by/3.0/This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
spellingShingle Research Article
Katariya, Chanchal
Jayakumar, ND
Kumar, Raghunandha
Effect of ascorbic acid on cellular respiration with mitochondrial reductase in gingival fibroblast
title Effect of ascorbic acid on cellular respiration with mitochondrial reductase in gingival fibroblast
title_full Effect of ascorbic acid on cellular respiration with mitochondrial reductase in gingival fibroblast
title_fullStr Effect of ascorbic acid on cellular respiration with mitochondrial reductase in gingival fibroblast
title_full_unstemmed Effect of ascorbic acid on cellular respiration with mitochondrial reductase in gingival fibroblast
title_short Effect of ascorbic acid on cellular respiration with mitochondrial reductase in gingival fibroblast
title_sort effect of ascorbic acid on cellular respiration with mitochondrial reductase in gingival fibroblast
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599683/
https://www.ncbi.nlm.nih.gov/pubmed/37886148
http://dx.doi.org/10.6026/97320630019552
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