Cargando…
Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice
Background: A mouse model of myocardial ischemia-reperfusion (I/R) is widely used to study myocardial ischemia-reperfusion injury (I/RI). However, few studies focus on the direct comparison of the extent of pathological events resulting from variant durations of ischemia and reperfusion process. Met...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599719/ https://www.ncbi.nlm.nih.gov/pubmed/37819785 http://dx.doi.org/10.18632/aging.205103 |
_version_ | 1785125825966768128 |
---|---|
author | Meng, Xiang-Min Yuan, Jing-Han Zhou, Zhen-Fang Feng, Qi-Pu Zhu, Bing-Mei |
author_facet | Meng, Xiang-Min Yuan, Jing-Han Zhou, Zhen-Fang Feng, Qi-Pu Zhu, Bing-Mei |
author_sort | Meng, Xiang-Min |
collection | PubMed |
description | Background: A mouse model of myocardial ischemia-reperfusion (I/R) is widely used to study myocardial ischemia-reperfusion injury (I/RI). However, few studies focus on the direct comparison of the extent of pathological events resulting from variant durations of ischemia and reperfusion process. Methods: A mouse model of I/RI was established by ligation and perfusion of the left anterior descending coronary artery (LAD), and the dynamic changes were recorded by electrocardiogram at different stages of I/R. Subsequently, reperfusion duration was used as a variable to directly compare the phenotypes of different myocardial injury degrees induced by 3 h, 6 h and 24 h reperfusion from myocardial infarct size, myocardial apoptosis, myocardial enzyme, and inflammatory cytokine levels. Results: All mice subjected to myocardial I/R surgery showed obvious myocardial infarction, extensive myocardial apoptosis, dynamic changes in serum myocardial enzyme and inflammatory cytokines, at least for the first 24 h of reperfusion. The infarct size and apoptosis rates gradually increased with the extension of reperfusion time. The peaks of serum myocardial enzyme and inflammatory cytokines occurred at 6 h and 3 h of reperfusion, respectively. We also established I/R mice models with 30 and 60 mins of ischemia. After 21 days of remodeling, longer periods of ischemia increased the degree of fibrosis and reduced cardiac function. Conclusions: In summary, we conclude that reperfusion durations of 3 h, 6 h, and 24 h induces different injury phenotypes in ischemia-reperfusion mouse model. At the same time, the ischemia duration before reperfusion also affects the degree of cardiac remodeling. |
format | Online Article Text |
id | pubmed-10599719 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-105997192023-10-26 Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice Meng, Xiang-Min Yuan, Jing-Han Zhou, Zhen-Fang Feng, Qi-Pu Zhu, Bing-Mei Aging (Albany NY) Research Paper Background: A mouse model of myocardial ischemia-reperfusion (I/R) is widely used to study myocardial ischemia-reperfusion injury (I/RI). However, few studies focus on the direct comparison of the extent of pathological events resulting from variant durations of ischemia and reperfusion process. Methods: A mouse model of I/RI was established by ligation and perfusion of the left anterior descending coronary artery (LAD), and the dynamic changes were recorded by electrocardiogram at different stages of I/R. Subsequently, reperfusion duration was used as a variable to directly compare the phenotypes of different myocardial injury degrees induced by 3 h, 6 h and 24 h reperfusion from myocardial infarct size, myocardial apoptosis, myocardial enzyme, and inflammatory cytokine levels. Results: All mice subjected to myocardial I/R surgery showed obvious myocardial infarction, extensive myocardial apoptosis, dynamic changes in serum myocardial enzyme and inflammatory cytokines, at least for the first 24 h of reperfusion. The infarct size and apoptosis rates gradually increased with the extension of reperfusion time. The peaks of serum myocardial enzyme and inflammatory cytokines occurred at 6 h and 3 h of reperfusion, respectively. We also established I/R mice models with 30 and 60 mins of ischemia. After 21 days of remodeling, longer periods of ischemia increased the degree of fibrosis and reduced cardiac function. Conclusions: In summary, we conclude that reperfusion durations of 3 h, 6 h, and 24 h induces different injury phenotypes in ischemia-reperfusion mouse model. At the same time, the ischemia duration before reperfusion also affects the degree of cardiac remodeling. Impact Journals 2023-10-10 /pmc/articles/PMC10599719/ /pubmed/37819785 http://dx.doi.org/10.18632/aging.205103 Text en Copyright: © 2023 Meng et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Meng, Xiang-Min Yuan, Jing-Han Zhou, Zhen-Fang Feng, Qi-Pu Zhu, Bing-Mei Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice |
title | Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice |
title_full | Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice |
title_fullStr | Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice |
title_full_unstemmed | Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice |
title_short | Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice |
title_sort | evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599719/ https://www.ncbi.nlm.nih.gov/pubmed/37819785 http://dx.doi.org/10.18632/aging.205103 |
work_keys_str_mv | AT mengxiangmin evaluationoftimedependentphenotypesofmyocardialischemiareperfusioninmice AT yuanjinghan evaluationoftimedependentphenotypesofmyocardialischemiareperfusioninmice AT zhouzhenfang evaluationoftimedependentphenotypesofmyocardialischemiareperfusioninmice AT fengqipu evaluationoftimedependentphenotypesofmyocardialischemiareperfusioninmice AT zhubingmei evaluationoftimedependentphenotypesofmyocardialischemiareperfusioninmice |