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Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice

Background: A mouse model of myocardial ischemia-reperfusion (I/R) is widely used to study myocardial ischemia-reperfusion injury (I/RI). However, few studies focus on the direct comparison of the extent of pathological events resulting from variant durations of ischemia and reperfusion process. Met...

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Autores principales: Meng, Xiang-Min, Yuan, Jing-Han, Zhou, Zhen-Fang, Feng, Qi-Pu, Zhu, Bing-Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599719/
https://www.ncbi.nlm.nih.gov/pubmed/37819785
http://dx.doi.org/10.18632/aging.205103
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author Meng, Xiang-Min
Yuan, Jing-Han
Zhou, Zhen-Fang
Feng, Qi-Pu
Zhu, Bing-Mei
author_facet Meng, Xiang-Min
Yuan, Jing-Han
Zhou, Zhen-Fang
Feng, Qi-Pu
Zhu, Bing-Mei
author_sort Meng, Xiang-Min
collection PubMed
description Background: A mouse model of myocardial ischemia-reperfusion (I/R) is widely used to study myocardial ischemia-reperfusion injury (I/RI). However, few studies focus on the direct comparison of the extent of pathological events resulting from variant durations of ischemia and reperfusion process. Methods: A mouse model of I/RI was established by ligation and perfusion of the left anterior descending coronary artery (LAD), and the dynamic changes were recorded by electrocardiogram at different stages of I/R. Subsequently, reperfusion duration was used as a variable to directly compare the phenotypes of different myocardial injury degrees induced by 3 h, 6 h and 24 h reperfusion from myocardial infarct size, myocardial apoptosis, myocardial enzyme, and inflammatory cytokine levels. Results: All mice subjected to myocardial I/R surgery showed obvious myocardial infarction, extensive myocardial apoptosis, dynamic changes in serum myocardial enzyme and inflammatory cytokines, at least for the first 24 h of reperfusion. The infarct size and apoptosis rates gradually increased with the extension of reperfusion time. The peaks of serum myocardial enzyme and inflammatory cytokines occurred at 6 h and 3 h of reperfusion, respectively. We also established I/R mice models with 30 and 60 mins of ischemia. After 21 days of remodeling, longer periods of ischemia increased the degree of fibrosis and reduced cardiac function. Conclusions: In summary, we conclude that reperfusion durations of 3 h, 6 h, and 24 h induces different injury phenotypes in ischemia-reperfusion mouse model. At the same time, the ischemia duration before reperfusion also affects the degree of cardiac remodeling.
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spelling pubmed-105997192023-10-26 Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice Meng, Xiang-Min Yuan, Jing-Han Zhou, Zhen-Fang Feng, Qi-Pu Zhu, Bing-Mei Aging (Albany NY) Research Paper Background: A mouse model of myocardial ischemia-reperfusion (I/R) is widely used to study myocardial ischemia-reperfusion injury (I/RI). However, few studies focus on the direct comparison of the extent of pathological events resulting from variant durations of ischemia and reperfusion process. Methods: A mouse model of I/RI was established by ligation and perfusion of the left anterior descending coronary artery (LAD), and the dynamic changes were recorded by electrocardiogram at different stages of I/R. Subsequently, reperfusion duration was used as a variable to directly compare the phenotypes of different myocardial injury degrees induced by 3 h, 6 h and 24 h reperfusion from myocardial infarct size, myocardial apoptosis, myocardial enzyme, and inflammatory cytokine levels. Results: All mice subjected to myocardial I/R surgery showed obvious myocardial infarction, extensive myocardial apoptosis, dynamic changes in serum myocardial enzyme and inflammatory cytokines, at least for the first 24 h of reperfusion. The infarct size and apoptosis rates gradually increased with the extension of reperfusion time. The peaks of serum myocardial enzyme and inflammatory cytokines occurred at 6 h and 3 h of reperfusion, respectively. We also established I/R mice models with 30 and 60 mins of ischemia. After 21 days of remodeling, longer periods of ischemia increased the degree of fibrosis and reduced cardiac function. Conclusions: In summary, we conclude that reperfusion durations of 3 h, 6 h, and 24 h induces different injury phenotypes in ischemia-reperfusion mouse model. At the same time, the ischemia duration before reperfusion also affects the degree of cardiac remodeling. Impact Journals 2023-10-10 /pmc/articles/PMC10599719/ /pubmed/37819785 http://dx.doi.org/10.18632/aging.205103 Text en Copyright: © 2023 Meng et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Meng, Xiang-Min
Yuan, Jing-Han
Zhou, Zhen-Fang
Feng, Qi-Pu
Zhu, Bing-Mei
Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice
title Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice
title_full Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice
title_fullStr Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice
title_full_unstemmed Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice
title_short Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice
title_sort evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599719/
https://www.ncbi.nlm.nih.gov/pubmed/37819785
http://dx.doi.org/10.18632/aging.205103
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