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Adiponectin‐expressing Treg‐containing T cell fraction inhibits tumor growth in orthotopically implanted triple‐negative breast cancer

BACKGROUND: In our previous study, we identified a population of adiponectin expressing regulatory T cells (Tregs) residing within thymic nurse cell complexes, which were capable of inhibiting the development of breast cancer in vitro. Triple‐negative breast cancer (TNBC) with no proper treatment at...

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Autores principales: Chikaishi, Wakana, Higashi, Toshiya, Hayashi, Hirokatsu, Hanamatsu, Yuki, Futamura, Manabu, Matsuhashi, Nobuhisa, Saigo, Chiemi, Takeuchi, Tamotsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Australia, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599968/
https://www.ncbi.nlm.nih.gov/pubmed/37674354
http://dx.doi.org/10.1111/1759-7714.15102
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author Chikaishi, Wakana
Higashi, Toshiya
Hayashi, Hirokatsu
Hanamatsu, Yuki
Futamura, Manabu
Matsuhashi, Nobuhisa
Saigo, Chiemi
Takeuchi, Tamotsu
author_facet Chikaishi, Wakana
Higashi, Toshiya
Hayashi, Hirokatsu
Hanamatsu, Yuki
Futamura, Manabu
Matsuhashi, Nobuhisa
Saigo, Chiemi
Takeuchi, Tamotsu
author_sort Chikaishi, Wakana
collection PubMed
description BACKGROUND: In our previous study, we identified a population of adiponectin expressing regulatory T cells (Tregs) residing within thymic nurse cell complexes, which were capable of inhibiting the development of breast cancer in vitro. Triple‐negative breast cancer (TNBC) with no proper treatment at present is characterized by the absence of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor‐2. In this study, we aimed to investigate the potential of a cultured T cell fraction comprising adiponectin‐expressing Tregs, referred to as A‐TregTF (adiponectin‐expressing Treg‐containing T cell fraction), in inhibiting the progression of TNBC in vivo. METHODS: The efficacy of a spontaneously expanding T cell fraction comprising adiponectin‐expressing Treg in inhibiting tumor growth was analyzed in a murine orthotopic 4 T1‐Luc TNBC model. RESULTS: The treatment with T cell fraction containing adiponectin‐expressing Tregs significantly inhibited the growth and metastasis of orthotopically transplanted 4 T1‐Luc tumor cells. Histopathological examination further revealed that the adiponectin‐expressing Tregs infiltrated the tumor tissue via a cell‐in‐cell mechanism and were found to be specifically localized around the necrotic areas. CONCLUSIONS: Based on our findings, the T cell fraction comprising adiponectin‐expressing Tregs, represents a potential candidate for adoptive cell therapy against TNBC.
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spelling pubmed-105999682023-10-27 Adiponectin‐expressing Treg‐containing T cell fraction inhibits tumor growth in orthotopically implanted triple‐negative breast cancer Chikaishi, Wakana Higashi, Toshiya Hayashi, Hirokatsu Hanamatsu, Yuki Futamura, Manabu Matsuhashi, Nobuhisa Saigo, Chiemi Takeuchi, Tamotsu Thorac Cancer Original Articles BACKGROUND: In our previous study, we identified a population of adiponectin expressing regulatory T cells (Tregs) residing within thymic nurse cell complexes, which were capable of inhibiting the development of breast cancer in vitro. Triple‐negative breast cancer (TNBC) with no proper treatment at present is characterized by the absence of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor‐2. In this study, we aimed to investigate the potential of a cultured T cell fraction comprising adiponectin‐expressing Tregs, referred to as A‐TregTF (adiponectin‐expressing Treg‐containing T cell fraction), in inhibiting the progression of TNBC in vivo. METHODS: The efficacy of a spontaneously expanding T cell fraction comprising adiponectin‐expressing Treg in inhibiting tumor growth was analyzed in a murine orthotopic 4 T1‐Luc TNBC model. RESULTS: The treatment with T cell fraction containing adiponectin‐expressing Tregs significantly inhibited the growth and metastasis of orthotopically transplanted 4 T1‐Luc tumor cells. Histopathological examination further revealed that the adiponectin‐expressing Tregs infiltrated the tumor tissue via a cell‐in‐cell mechanism and were found to be specifically localized around the necrotic areas. CONCLUSIONS: Based on our findings, the T cell fraction comprising adiponectin‐expressing Tregs, represents a potential candidate for adoptive cell therapy against TNBC. John Wiley & Sons Australia, Ltd 2023-09-06 /pmc/articles/PMC10599968/ /pubmed/37674354 http://dx.doi.org/10.1111/1759-7714.15102 Text en © 2023 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Chikaishi, Wakana
Higashi, Toshiya
Hayashi, Hirokatsu
Hanamatsu, Yuki
Futamura, Manabu
Matsuhashi, Nobuhisa
Saigo, Chiemi
Takeuchi, Tamotsu
Adiponectin‐expressing Treg‐containing T cell fraction inhibits tumor growth in orthotopically implanted triple‐negative breast cancer
title Adiponectin‐expressing Treg‐containing T cell fraction inhibits tumor growth in orthotopically implanted triple‐negative breast cancer
title_full Adiponectin‐expressing Treg‐containing T cell fraction inhibits tumor growth in orthotopically implanted triple‐negative breast cancer
title_fullStr Adiponectin‐expressing Treg‐containing T cell fraction inhibits tumor growth in orthotopically implanted triple‐negative breast cancer
title_full_unstemmed Adiponectin‐expressing Treg‐containing T cell fraction inhibits tumor growth in orthotopically implanted triple‐negative breast cancer
title_short Adiponectin‐expressing Treg‐containing T cell fraction inhibits tumor growth in orthotopically implanted triple‐negative breast cancer
title_sort adiponectin‐expressing treg‐containing t cell fraction inhibits tumor growth in orthotopically implanted triple‐negative breast cancer
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599968/
https://www.ncbi.nlm.nih.gov/pubmed/37674354
http://dx.doi.org/10.1111/1759-7714.15102
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