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CircPKM2 aggravates the progression of non‐small cell lung cancer by regulating MTDH via miR‐1298‐5p
BACKGROUND: Non‐small cell lung cancer (NSCLC) is the most common type of lung cancer with high morbidity and mortality. The role of dysregulated circular RNAs (circRNAs) in human diseases are receiving more and more attention. In this study, we focused on the role and mechanism of circPKM2 in the p...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons Australia, Ltd
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599976/ https://www.ncbi.nlm.nih.gov/pubmed/37675591 http://dx.doi.org/10.1111/1759-7714.15092 |
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author | Gao, Shuhua Gao, Tingting Feng, Li Li, Haixia Dong, Guogang Yang, Shan |
author_facet | Gao, Shuhua Gao, Tingting Feng, Li Li, Haixia Dong, Guogang Yang, Shan |
author_sort | Gao, Shuhua |
collection | PubMed |
description | BACKGROUND: Non‐small cell lung cancer (NSCLC) is the most common type of lung cancer with high morbidity and mortality. The role of dysregulated circular RNAs (circRNAs) in human diseases are receiving more and more attention. In this study, we focused on the role and mechanism of circPKM2 in the progression of NSCLC. METHODS: The expression levels of circPKM2, microRNA‐1298‐5p (miR‐1298‐5p) and metadherin (MTDH) in NSCLC were measured by real‐time quantitative PCR (qRT‐PCR) or Western blot. Cell counting kit‐8 (CCK‐8), colony formation, 5‐ethynyl‐2′‐deoxyuridine (EdU) staining, flow cytometry, transwell and tube formation assays were conducted to evaluate the effects of circPKM2 on malignant phenotypes of NSCLC. Western blot was used to measure related marker protein levels. RESULTS: CircPKM2 and MTDH were highly expressed in NSCLC tissues and cells, while miR‐1298‐5p was downregulated. CircPKM2 knockdown effectively suppressed cell proliferation, migration, invasion and tube formation whereas induced apoptosis in vitro. CircPKM2 had a potential targeting site with miR‐1298‐5p and negatively regulated the expression of miR‐1298‐5p. MiR‐1298‐5p inhibitor reversed the effect of circPKM2 knockdown on the progression of NSCLC. CircPKM2 induced MTDH expression via sponging miR‐1298‐5p to promote the progression of NSCLC. MiR‐1298‐5p directly targeted MTDH, and the addition of MTDH partially attenuated the inhibition of miR‐1298‐5p on the progression of NSCLC. In addition, the downregulation of circPKM2 significantly slowed down the growth of xenograft tumors in vivo. CONCLUSION: Our findings demonstrated that circPKM2 mediated NSCLC progression via regulating miR‐1298‐5p/MTDH axis, providing a novel therapeutic target for NSCLC. |
format | Online Article Text |
id | pubmed-10599976 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley & Sons Australia, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-105999762023-10-27 CircPKM2 aggravates the progression of non‐small cell lung cancer by regulating MTDH via miR‐1298‐5p Gao, Shuhua Gao, Tingting Feng, Li Li, Haixia Dong, Guogang Yang, Shan Thorac Cancer Original Articles BACKGROUND: Non‐small cell lung cancer (NSCLC) is the most common type of lung cancer with high morbidity and mortality. The role of dysregulated circular RNAs (circRNAs) in human diseases are receiving more and more attention. In this study, we focused on the role and mechanism of circPKM2 in the progression of NSCLC. METHODS: The expression levels of circPKM2, microRNA‐1298‐5p (miR‐1298‐5p) and metadherin (MTDH) in NSCLC were measured by real‐time quantitative PCR (qRT‐PCR) or Western blot. Cell counting kit‐8 (CCK‐8), colony formation, 5‐ethynyl‐2′‐deoxyuridine (EdU) staining, flow cytometry, transwell and tube formation assays were conducted to evaluate the effects of circPKM2 on malignant phenotypes of NSCLC. Western blot was used to measure related marker protein levels. RESULTS: CircPKM2 and MTDH were highly expressed in NSCLC tissues and cells, while miR‐1298‐5p was downregulated. CircPKM2 knockdown effectively suppressed cell proliferation, migration, invasion and tube formation whereas induced apoptosis in vitro. CircPKM2 had a potential targeting site with miR‐1298‐5p and negatively regulated the expression of miR‐1298‐5p. MiR‐1298‐5p inhibitor reversed the effect of circPKM2 knockdown on the progression of NSCLC. CircPKM2 induced MTDH expression via sponging miR‐1298‐5p to promote the progression of NSCLC. MiR‐1298‐5p directly targeted MTDH, and the addition of MTDH partially attenuated the inhibition of miR‐1298‐5p on the progression of NSCLC. In addition, the downregulation of circPKM2 significantly slowed down the growth of xenograft tumors in vivo. CONCLUSION: Our findings demonstrated that circPKM2 mediated NSCLC progression via regulating miR‐1298‐5p/MTDH axis, providing a novel therapeutic target for NSCLC. John Wiley & Sons Australia, Ltd 2023-09-07 /pmc/articles/PMC10599976/ /pubmed/37675591 http://dx.doi.org/10.1111/1759-7714.15092 Text en © 2023 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Gao, Shuhua Gao, Tingting Feng, Li Li, Haixia Dong, Guogang Yang, Shan CircPKM2 aggravates the progression of non‐small cell lung cancer by regulating MTDH via miR‐1298‐5p |
title |
CircPKM2 aggravates the progression of non‐small cell lung cancer by regulating MTDH via miR‐1298‐5p |
title_full |
CircPKM2 aggravates the progression of non‐small cell lung cancer by regulating MTDH via miR‐1298‐5p |
title_fullStr |
CircPKM2 aggravates the progression of non‐small cell lung cancer by regulating MTDH via miR‐1298‐5p |
title_full_unstemmed |
CircPKM2 aggravates the progression of non‐small cell lung cancer by regulating MTDH via miR‐1298‐5p |
title_short |
CircPKM2 aggravates the progression of non‐small cell lung cancer by regulating MTDH via miR‐1298‐5p |
title_sort | circpkm2 aggravates the progression of non‐small cell lung cancer by regulating mtdh via mir‐1298‐5p |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599976/ https://www.ncbi.nlm.nih.gov/pubmed/37675591 http://dx.doi.org/10.1111/1759-7714.15092 |
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