An accurate, reliable, and universal qPCR method to identify homozygous single insert T-DNA with the example of transgenic rice

Early determination of transgenic plants that are homozygous for a single locus T-DNA insert is highly desirable in most fundamental and applied transgenic research. This study aimed to build on an accurate, rapid, and reliable quantitative real-time PCR (qPCR) method to fast-track the development o...

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Detalles Bibliográficos
Autores principales: Tran, Hai Thanh, Schramm, Carly, Huynh, My-my, Shavrukov, Yuri, Stangoulis, James C. R., Jenkins, Colin L. D., Anderson, Peter A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10600460/
https://www.ncbi.nlm.nih.gov/pubmed/37900763
http://dx.doi.org/10.3389/fpls.2023.1221790

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10600460/
https://www.ncbi.nlm.nih.gov/pubmed/37900763
http://dx.doi.org/10.3389/fpls.2023.1221790

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