Cargando…
An accurate, reliable, and universal qPCR method to identify homozygous single insert T-DNA with the example of transgenic rice
Early determination of transgenic plants that are homozygous for a single locus T-DNA insert is highly desirable in most fundamental and applied transgenic research. This study aimed to build on an accurate, rapid, and reliable quantitative real-time PCR (qPCR) method to fast-track the development o...
Autores principales: | Tran, Hai Thanh, Schramm, Carly, Huynh, My-my, Shavrukov, Yuri, Stangoulis, James C. R., Jenkins, Colin L. D., Anderson, Peter A. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10600460/ https://www.ncbi.nlm.nih.gov/pubmed/37900763 http://dx.doi.org/10.3389/fpls.2023.1221790 |
Ejemplares similares
-
Modified “Allele-Specific qPCR” Method for SNP Genotyping Based on FRET
por: Kalendar, Ruslan, et al.
Publicado: (2022) -
Selection and testing of reference genes for accurate RT-qPCR in rice seedlings under iron toxicity
por: dos Santos, Fabiane Igansi de Castro, et al.
Publicado: (2018) -
Rapid and accurate quantification of isomiRs by RT-qPCR
por: Franco, Sandra, et al.
Publicado: (2022) -
A biolistic method for high-throughput production of transgenic wheat plants with single gene insertions
por: Ismagul, Ainur, et al.
Publicado: (2018) -
Accurate RT-qPCR gene expression analysis on cell culture lysates
por: Van Peer, Gert, et al.
Publicado: (2012)