Record of Cryptosporidium serpentis from Goniurosaurus kuroiwae sengokui (Reptilia, Squamata, Eublepharidae) in Tokashikijima, Okinawa Prefecture, Japan

In reptiles, infection with Cryptosporidium parasite can be lethal, especially in species of family Eublepharidae such as Eublepharis macularius and Goniurosaurus splendes species. We investigated the presence of Cryptosporidium spp. in wild reptiles, especially Goniurosaurus spp., in five islands of Japan. Nested PCR was performed to detect Cryptosporidium spp. in 38 fecal samples of 9 reptile species from five islands of Japan, and 3 (3/8) positive samples of Goniurosaurus kuroiwae sengokui from Tokashikijima, Okinawa Prefecture were detected. A BLAST search of the detected gene sequences revealed that the all 3 samples were identified as Cryptosporidium serpentis, a reptile-specific Cryptosporidium parasite. This is the first record of Cryptosporidium from a wild Goniurosaurus species in Japan and worldwide.