Cargando…

Podoplanin promotes cell proliferation, survival, and migration of canine non-tonsillar squamous cell carcinoma

Podoplanin (PDPN) is a prognostic factor and is involved in several mechanisms of tumor progression in human squamous cell carcinoma (SCC). Canine non-tonsillar SCC (NTSCC) is a common oral tumor in dogs and has a highly invasive characteristic. In this study, we investigated the function of PDPN in...

Descripción completa

Detalles Bibliográficos
Autores principales: SHINADA, Masahiro, KATO, Daiki, TSUBOI, Masaya, IKEDA, Namiko, AOKI, Susumu, IGUCHI, Takaaki, LI, Toshio, KODERA, Yuka, OTA, Ryosuke, KOSEKI, Shoma, SHIBAHARA, Hayato, TAKAHASHI, Yosuke, HASHIMOTO, Yuko, CHAMBERS, James K, UCHIDA, Kazuyuki, NOGUCHI, Shunsuke, KATO, Yukinari, NISHIMURA, Ryohei, NAKAGAWA, Takayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10600541/
https://www.ncbi.nlm.nih.gov/pubmed/37544715
http://dx.doi.org/10.1292/jvms.23-0062
_version_ 1785126009284067328
author SHINADA, Masahiro
KATO, Daiki
TSUBOI, Masaya
IKEDA, Namiko
AOKI, Susumu
IGUCHI, Takaaki
LI, Toshio
KODERA, Yuka
OTA, Ryosuke
KOSEKI, Shoma
SHIBAHARA, Hayato
TAKAHASHI, Yosuke
HASHIMOTO, Yuko
CHAMBERS, James K
UCHIDA, Kazuyuki
NOGUCHI, Shunsuke
KATO, Yukinari
NISHIMURA, Ryohei
NAKAGAWA, Takayuki
author_facet SHINADA, Masahiro
KATO, Daiki
TSUBOI, Masaya
IKEDA, Namiko
AOKI, Susumu
IGUCHI, Takaaki
LI, Toshio
KODERA, Yuka
OTA, Ryosuke
KOSEKI, Shoma
SHIBAHARA, Hayato
TAKAHASHI, Yosuke
HASHIMOTO, Yuko
CHAMBERS, James K
UCHIDA, Kazuyuki
NOGUCHI, Shunsuke
KATO, Yukinari
NISHIMURA, Ryohei
NAKAGAWA, Takayuki
author_sort SHINADA, Masahiro
collection PubMed
description Podoplanin (PDPN) is a prognostic factor and is involved in several mechanisms of tumor progression in human squamous cell carcinoma (SCC). Canine non-tonsillar SCC (NTSCC) is a common oral tumor in dogs and has a highly invasive characteristic. In this study, we investigated the function of PDPN in canine NTSCC. In canine NTSCC clinical samples, PDPN overexpression was observed in 80% of dogs with NTSCC, and PDPN expression was related to ki67 expression. In PDPN knocked-out canine NTSCC cells, cell proliferation, cancer stemness, and migration were suppressed. As the mechanism of PDPN-mediated cell proliferation, PDPN knocked-out induced apoptosis and G2/M cell cycle arrest in canine NTSCC cells. These findings suggest that PDPN promotes tumor malignancies and may be a novel biomarker and therapeutic target for canine NTSCC.
format Online
Article
Text
id pubmed-10600541
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher The Japanese Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-106005412023-10-27 Podoplanin promotes cell proliferation, survival, and migration of canine non-tonsillar squamous cell carcinoma SHINADA, Masahiro KATO, Daiki TSUBOI, Masaya IKEDA, Namiko AOKI, Susumu IGUCHI, Takaaki LI, Toshio KODERA, Yuka OTA, Ryosuke KOSEKI, Shoma SHIBAHARA, Hayato TAKAHASHI, Yosuke HASHIMOTO, Yuko CHAMBERS, James K UCHIDA, Kazuyuki NOGUCHI, Shunsuke KATO, Yukinari NISHIMURA, Ryohei NAKAGAWA, Takayuki J Vet Med Sci Surgery Podoplanin (PDPN) is a prognostic factor and is involved in several mechanisms of tumor progression in human squamous cell carcinoma (SCC). Canine non-tonsillar SCC (NTSCC) is a common oral tumor in dogs and has a highly invasive characteristic. In this study, we investigated the function of PDPN in canine NTSCC. In canine NTSCC clinical samples, PDPN overexpression was observed in 80% of dogs with NTSCC, and PDPN expression was related to ki67 expression. In PDPN knocked-out canine NTSCC cells, cell proliferation, cancer stemness, and migration were suppressed. As the mechanism of PDPN-mediated cell proliferation, PDPN knocked-out induced apoptosis and G2/M cell cycle arrest in canine NTSCC cells. These findings suggest that PDPN promotes tumor malignancies and may be a novel biomarker and therapeutic target for canine NTSCC. The Japanese Society of Veterinary Science 2023-08-04 2023-10 /pmc/articles/PMC10600541/ /pubmed/37544715 http://dx.doi.org/10.1292/jvms.23-0062 Text en ©2023 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Surgery
SHINADA, Masahiro
KATO, Daiki
TSUBOI, Masaya
IKEDA, Namiko
AOKI, Susumu
IGUCHI, Takaaki
LI, Toshio
KODERA, Yuka
OTA, Ryosuke
KOSEKI, Shoma
SHIBAHARA, Hayato
TAKAHASHI, Yosuke
HASHIMOTO, Yuko
CHAMBERS, James K
UCHIDA, Kazuyuki
NOGUCHI, Shunsuke
KATO, Yukinari
NISHIMURA, Ryohei
NAKAGAWA, Takayuki
Podoplanin promotes cell proliferation, survival, and migration of canine non-tonsillar squamous cell carcinoma
title Podoplanin promotes cell proliferation, survival, and migration of canine non-tonsillar squamous cell carcinoma
title_full Podoplanin promotes cell proliferation, survival, and migration of canine non-tonsillar squamous cell carcinoma
title_fullStr Podoplanin promotes cell proliferation, survival, and migration of canine non-tonsillar squamous cell carcinoma
title_full_unstemmed Podoplanin promotes cell proliferation, survival, and migration of canine non-tonsillar squamous cell carcinoma
title_short Podoplanin promotes cell proliferation, survival, and migration of canine non-tonsillar squamous cell carcinoma
title_sort podoplanin promotes cell proliferation, survival, and migration of canine non-tonsillar squamous cell carcinoma
topic Surgery
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10600541/
https://www.ncbi.nlm.nih.gov/pubmed/37544715
http://dx.doi.org/10.1292/jvms.23-0062
work_keys_str_mv AT shinadamasahiro podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT katodaiki podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT tsuboimasaya podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT ikedanamiko podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT aokisusumu podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT iguchitakaaki podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT litoshio podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT koderayuka podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT otaryosuke podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT kosekishoma podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT shibaharahayato podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT takahashiyosuke podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT hashimotoyuko podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT chambersjamesk podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT uchidakazuyuki podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT noguchishunsuke podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT katoyukinari podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT nishimuraryohei podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma
AT nakagawatakayuki podoplaninpromotescellproliferationsurvivalandmigrationofcaninenontonsillarsquamouscellcarcinoma