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Covalent functionalisation controlled by molecular design for the aptameric recognition of serotonin in graphene-based field-effect transistors

In the last decade, solution-gated graphene field effect transistors (GFETs) showed their versatility in the development of a miniaturized multiplexed platform for electrophysiological recordings and sensing. Due to their working mechanism, the surface functionalisation and immobilisation of recepto...

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Autores principales: Wetzl, Cecilia, Brosel-Oliu, Sergi, Carini, Marco, Di Silvio, Desiré, Illa, Xavi, Villa, Rosa, Guimera, Anton, Prats-Alfonso, Elisabet, Prato, Maurizio, Criado, Alejandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10600654/
https://www.ncbi.nlm.nih.gov/pubmed/37789811
http://dx.doi.org/10.1039/d3nr04153k
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author Wetzl, Cecilia
Brosel-Oliu, Sergi
Carini, Marco
Di Silvio, Desiré
Illa, Xavi
Villa, Rosa
Guimera, Anton
Prats-Alfonso, Elisabet
Prato, Maurizio
Criado, Alejandro
author_facet Wetzl, Cecilia
Brosel-Oliu, Sergi
Carini, Marco
Di Silvio, Desiré
Illa, Xavi
Villa, Rosa
Guimera, Anton
Prats-Alfonso, Elisabet
Prato, Maurizio
Criado, Alejandro
author_sort Wetzl, Cecilia
collection PubMed
description In the last decade, solution-gated graphene field effect transistors (GFETs) showed their versatility in the development of a miniaturized multiplexed platform for electrophysiological recordings and sensing. Due to their working mechanism, the surface functionalisation and immobilisation of receptors are pivotal to ensure the proper functioning of devices. Herein, we present a controlled covalent functionalisation strategy based on molecular design and electrochemical triggering, which provide a monolayer-like functionalisation of micro-GFET arrays retaining the electronic properties of graphenes. The functionalisation layer as a receptor was then employed as the linker for serotonin aptamer conjugation. The micro-GFET arrays display sensitivity toward the target analyte in the micromolar range in a physiological buffer (PBS 10 mM). The sensor allows the in-flow real-time monitoring of serotonin transient concentrations with fast and reversible responses.
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spelling pubmed-106006542023-10-27 Covalent functionalisation controlled by molecular design for the aptameric recognition of serotonin in graphene-based field-effect transistors Wetzl, Cecilia Brosel-Oliu, Sergi Carini, Marco Di Silvio, Desiré Illa, Xavi Villa, Rosa Guimera, Anton Prats-Alfonso, Elisabet Prato, Maurizio Criado, Alejandro Nanoscale Chemistry In the last decade, solution-gated graphene field effect transistors (GFETs) showed their versatility in the development of a miniaturized multiplexed platform for electrophysiological recordings and sensing. Due to their working mechanism, the surface functionalisation and immobilisation of receptors are pivotal to ensure the proper functioning of devices. Herein, we present a controlled covalent functionalisation strategy based on molecular design and electrochemical triggering, which provide a monolayer-like functionalisation of micro-GFET arrays retaining the electronic properties of graphenes. The functionalisation layer as a receptor was then employed as the linker for serotonin aptamer conjugation. The micro-GFET arrays display sensitivity toward the target analyte in the micromolar range in a physiological buffer (PBS 10 mM). The sensor allows the in-flow real-time monitoring of serotonin transient concentrations with fast and reversible responses. The Royal Society of Chemistry 2023-09-19 /pmc/articles/PMC10600654/ /pubmed/37789811 http://dx.doi.org/10.1039/d3nr04153k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Wetzl, Cecilia
Brosel-Oliu, Sergi
Carini, Marco
Di Silvio, Desiré
Illa, Xavi
Villa, Rosa
Guimera, Anton
Prats-Alfonso, Elisabet
Prato, Maurizio
Criado, Alejandro
Covalent functionalisation controlled by molecular design for the aptameric recognition of serotonin in graphene-based field-effect transistors
title Covalent functionalisation controlled by molecular design for the aptameric recognition of serotonin in graphene-based field-effect transistors
title_full Covalent functionalisation controlled by molecular design for the aptameric recognition of serotonin in graphene-based field-effect transistors
title_fullStr Covalent functionalisation controlled by molecular design for the aptameric recognition of serotonin in graphene-based field-effect transistors
title_full_unstemmed Covalent functionalisation controlled by molecular design for the aptameric recognition of serotonin in graphene-based field-effect transistors
title_short Covalent functionalisation controlled by molecular design for the aptameric recognition of serotonin in graphene-based field-effect transistors
title_sort covalent functionalisation controlled by molecular design for the aptameric recognition of serotonin in graphene-based field-effect transistors
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10600654/
https://www.ncbi.nlm.nih.gov/pubmed/37789811
http://dx.doi.org/10.1039/d3nr04153k
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