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Determinants of motor neuron functional subtypes important for locomotor speed
Locomotion requires precise control of the strength and speed of muscle contraction and is achieved by recruiting functionally distinct subtypes of motor neurons (MNs). MNs are essential to movement and differentially susceptible in disease, but little is known about how MNs acquire functional subty...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10600875/ https://www.ncbi.nlm.nih.gov/pubmed/37676768 http://dx.doi.org/10.1016/j.celrep.2023.113049 |
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author | D’Elia, Kristen P. Hameedy, Hanna Goldblatt, Dena Frazel, Paul Kriese, Mercer Zhu, Yunlu Hamling, Kyla R. Kawakami, Koichi Liddelow, Shane A. Schoppik, David Dasen, Jeremy S. |
author_facet | D’Elia, Kristen P. Hameedy, Hanna Goldblatt, Dena Frazel, Paul Kriese, Mercer Zhu, Yunlu Hamling, Kyla R. Kawakami, Koichi Liddelow, Shane A. Schoppik, David Dasen, Jeremy S. |
author_sort | D’Elia, Kristen P. |
collection | PubMed |
description | Locomotion requires precise control of the strength and speed of muscle contraction and is achieved by recruiting functionally distinct subtypes of motor neurons (MNs). MNs are essential to movement and differentially susceptible in disease, but little is known about how MNs acquire functional subtype-specific features during development. Using single-cell RNA profiling in embryonic and larval zebrafish, we identify novel and conserved molecular signatures for MN functional subtypes and identify genes expressed in both early post-mitotic and mature MNs. Assessing MN development in genetic mutants, we define a molecular program essential for MN functional subtype specification. Two evolutionarily conserved transcription factors, Prdm16 and Mecom, are both functional subtype-specific determinants integral for fast MN development. Loss of prdm16 or mecom causes fast MNs to develop transcriptional profiles and innervation similar to slow MNs. These results reveal the molecular diversity of vertebrate axial MNs and demonstrate that functional subtypes are specified through intrinsic transcriptional codes. |
format | Online Article Text |
id | pubmed-10600875 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
record_format | MEDLINE/PubMed |
spelling | pubmed-106008752023-10-26 Determinants of motor neuron functional subtypes important for locomotor speed D’Elia, Kristen P. Hameedy, Hanna Goldblatt, Dena Frazel, Paul Kriese, Mercer Zhu, Yunlu Hamling, Kyla R. Kawakami, Koichi Liddelow, Shane A. Schoppik, David Dasen, Jeremy S. Cell Rep Article Locomotion requires precise control of the strength and speed of muscle contraction and is achieved by recruiting functionally distinct subtypes of motor neurons (MNs). MNs are essential to movement and differentially susceptible in disease, but little is known about how MNs acquire functional subtype-specific features during development. Using single-cell RNA profiling in embryonic and larval zebrafish, we identify novel and conserved molecular signatures for MN functional subtypes and identify genes expressed in both early post-mitotic and mature MNs. Assessing MN development in genetic mutants, we define a molecular program essential for MN functional subtype specification. Two evolutionarily conserved transcription factors, Prdm16 and Mecom, are both functional subtype-specific determinants integral for fast MN development. Loss of prdm16 or mecom causes fast MNs to develop transcriptional profiles and innervation similar to slow MNs. These results reveal the molecular diversity of vertebrate axial MNs and demonstrate that functional subtypes are specified through intrinsic transcriptional codes. 2023-09-26 2023-09-06 /pmc/articles/PMC10600875/ /pubmed/37676768 http://dx.doi.org/10.1016/j.celrep.2023.113049 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ). |
spellingShingle | Article D’Elia, Kristen P. Hameedy, Hanna Goldblatt, Dena Frazel, Paul Kriese, Mercer Zhu, Yunlu Hamling, Kyla R. Kawakami, Koichi Liddelow, Shane A. Schoppik, David Dasen, Jeremy S. Determinants of motor neuron functional subtypes important for locomotor speed |
title | Determinants of motor neuron functional subtypes important for locomotor speed |
title_full | Determinants of motor neuron functional subtypes important for locomotor speed |
title_fullStr | Determinants of motor neuron functional subtypes important for locomotor speed |
title_full_unstemmed | Determinants of motor neuron functional subtypes important for locomotor speed |
title_short | Determinants of motor neuron functional subtypes important for locomotor speed |
title_sort | determinants of motor neuron functional subtypes important for locomotor speed |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10600875/ https://www.ncbi.nlm.nih.gov/pubmed/37676768 http://dx.doi.org/10.1016/j.celrep.2023.113049 |
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