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Design and Synthesis of d-Ala-d-Ala Silica Gel for a Binding Mode-Based Physicochemical Screening Method
[Image: see text] Vancomycin is a potent and broad-spectrum antibiotic that binds to the d-Ala-d-Ala moiety of the growing bacterial cell wall and kills bacteria. This fascinating binding model prompted us to design and synthesize d-Ala-d-Ala silica gels for the establishment of a new physicochemica...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical Society
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10601077/ https://www.ncbi.nlm.nih.gov/pubmed/37901494 http://dx.doi.org/10.1021/acsomega.3c03837 |
| _version_ | 1785126119355187200 |
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| author | Kimishima, Aoi Suzuki, Yuki Kosaka, Shohei Honsho, Masako Honma, Sota Sakai, Kazunari Tsutsumi, Hayama Inahashi, Yuki Watanabe, Yoshihiro Iwatsuki, Masato Asami, Yukihiro Nakashima, Takuji |
| author_facet | Kimishima, Aoi Suzuki, Yuki Kosaka, Shohei Honsho, Masako Honma, Sota Sakai, Kazunari Tsutsumi, Hayama Inahashi, Yuki Watanabe, Yoshihiro Iwatsuki, Masato Asami, Yukihiro Nakashima, Takuji |
| author_sort | Kimishima, Aoi |
| collection | PubMed |
| description | [Image: see text] Vancomycin is a potent and broad-spectrum antibiotic that binds to the d-Ala-d-Ala moiety of the growing bacterial cell wall and kills bacteria. This fascinating binding model prompted us to design and synthesize d-Ala-d-Ala silica gels for the establishment of a new physicochemical (PC) screening method. In this report, we confirmed that vancomycin binds to d-Ala-d-Ala silica gel and can be eluted with MeOH containing 50 mM TFA. Finally, d-Ala-d-Ala silica gel enables to purify vancomycin from the culture broth of a vancomycin-producing strain, Amycolatopsis orientalis. |
| format | Online Article Text |
| id | pubmed-10601077 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | American Chemical Society |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-106010772023-10-27 Design and Synthesis of d-Ala-d-Ala Silica Gel for a Binding Mode-Based Physicochemical Screening Method Kimishima, Aoi Suzuki, Yuki Kosaka, Shohei Honsho, Masako Honma, Sota Sakai, Kazunari Tsutsumi, Hayama Inahashi, Yuki Watanabe, Yoshihiro Iwatsuki, Masato Asami, Yukihiro Nakashima, Takuji ACS Omega [Image: see text] Vancomycin is a potent and broad-spectrum antibiotic that binds to the d-Ala-d-Ala moiety of the growing bacterial cell wall and kills bacteria. This fascinating binding model prompted us to design and synthesize d-Ala-d-Ala silica gels for the establishment of a new physicochemical (PC) screening method. In this report, we confirmed that vancomycin binds to d-Ala-d-Ala silica gel and can be eluted with MeOH containing 50 mM TFA. Finally, d-Ala-d-Ala silica gel enables to purify vancomycin from the culture broth of a vancomycin-producing strain, Amycolatopsis orientalis. American Chemical Society 2023-10-09 /pmc/articles/PMC10601077/ /pubmed/37901494 http://dx.doi.org/10.1021/acsomega.3c03837 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
| spellingShingle | Kimishima, Aoi Suzuki, Yuki Kosaka, Shohei Honsho, Masako Honma, Sota Sakai, Kazunari Tsutsumi, Hayama Inahashi, Yuki Watanabe, Yoshihiro Iwatsuki, Masato Asami, Yukihiro Nakashima, Takuji Design and Synthesis of d-Ala-d-Ala Silica Gel for a Binding Mode-Based Physicochemical Screening Method |
| title | Design and Synthesis
of d-Ala-d-Ala Silica Gel for
a Binding Mode-Based Physicochemical
Screening Method |
| title_full | Design and Synthesis
of d-Ala-d-Ala Silica Gel for
a Binding Mode-Based Physicochemical
Screening Method |
| title_fullStr | Design and Synthesis
of d-Ala-d-Ala Silica Gel for
a Binding Mode-Based Physicochemical
Screening Method |
| title_full_unstemmed | Design and Synthesis
of d-Ala-d-Ala Silica Gel for
a Binding Mode-Based Physicochemical
Screening Method |
| title_short | Design and Synthesis
of d-Ala-d-Ala Silica Gel for
a Binding Mode-Based Physicochemical
Screening Method |
| title_sort | design and synthesis
of d-ala-d-ala silica gel for
a binding mode-based physicochemical
screening method |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10601077/ https://www.ncbi.nlm.nih.gov/pubmed/37901494 http://dx.doi.org/10.1021/acsomega.3c03837 |
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