Cargando…
Predicting Successful Hematopoietic Stem Cell Collection in Healthy Allogeneic Donors
INTRODUCTION: Collection of peripheral blood stem cells (PBSCs) from healthy donors is a well-established process. We aimed to identify factors predictive of successful CD34+ PBSC collection and established a formula capable of predicting CD34+ cell yield. METHODS: We retrospectively evaluated 588 h...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
S. Karger AG
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10601602/ https://www.ncbi.nlm.nih.gov/pubmed/37899994 http://dx.doi.org/10.1159/000531236 |
_version_ | 1785126229832105984 |
---|---|
author | Kayser, Sabine Schlenk, Richard F. Steiner, Marcus Klüter, Harald Wuchter, Patrick |
author_facet | Kayser, Sabine Schlenk, Richard F. Steiner, Marcus Klüter, Harald Wuchter, Patrick |
author_sort | Kayser, Sabine |
collection | PubMed |
description | INTRODUCTION: Collection of peripheral blood stem cells (PBSCs) from healthy donors is a well-established process. We aimed to identify factors predictive of successful CD34+ PBSC collection and established a formula capable of predicting CD34+ cell yield. METHODS: We retrospectively evaluated 588 healthy adult donors (median age 29 years, range 18–69 years) at our institution from 2017 to 2022. The predicted minimal number of CD34+ cells was calculated as follows: (peripheral CD34+ cells/µL × adjusted collection efficiency of 30%) × total liters processed. This formula was further modified according to donor and recipient body weight (BW). RESULTS: Median total collection was 8.0 × 10(6) CD34+ cells/kg BW (range 1.0–47.1 × 10(6) cells/kg BW) with 522 donors (89%) collecting ≥5.0 × 10(6) cells/kg of recipient BW. A second leukapheresis (LP) was performed in 49 donors. Need for two LPs was more common in female donors (OR 6.68, 95% CI, 2.62–17.05; p < 0.001), donors with higher age (OR for 10 years difference 1.53, 95% CI, 1.15–2.03, p = 0.003), donors with WBC count <30 × 10(9)/L after 5 days of granulocyte-colony stimulating factor (G-CSF) stimulation (OR, 4.33; 95% CI, 1.59–11.83; p = 0.004), and a donor/recipient weight ratio <1 (OR 6.21, 95% CI, 2.69–14.34; p < 0.001). Predictive factors for optimal LP (i.e., ≥5.0 × 10(6) CD34+ cells/kg of recipient BW) were peripheral blood (PB) CD34+ cell count >50/µL (OR 12.82, range 6.34–25.92, p < 0.001), male donor (OR 2.77, range 1.06–7.23, p = 0.04), and a donor/recipient weight ratio >1 (OR 3.12, range 1.57–6.24, p = 0.001). WBC, platelets, hemoglobin, and age had no significant predictive value. Predicted versus observed number of CD34+ cells/kg BW collected demonstrated a very strong linear correlation (r = 0.925, 95% CI, 0.912–0.936, p < 0.0001). CONCLUSIONS: Of the routinely monitored indicators in PBSC donors, CD34+ cell count in PB is the most important factor in predicting G-CSF-induced PBSC yields. Higher age, female sex, WBC <30 × 10(9)/L, and a donor/recipient weight ratio <1 are useful indicators for identifying suboptimal mobilizers. The modified formula has shown successful and consistent performance in the prediction of key outcome measures including the minimum CD34+ cell collection, determination of the required length of apheresis, and whether a second day of PBSC collection was necessary to achieve the respective collection goal. |
format | Online Article Text |
id | pubmed-10601602 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | S. Karger AG |
record_format | MEDLINE/PubMed |
spelling | pubmed-106016022023-10-27 Predicting Successful Hematopoietic Stem Cell Collection in Healthy Allogeneic Donors Kayser, Sabine Schlenk, Richard F. Steiner, Marcus Klüter, Harald Wuchter, Patrick Transfus Med Hemother Research Article INTRODUCTION: Collection of peripheral blood stem cells (PBSCs) from healthy donors is a well-established process. We aimed to identify factors predictive of successful CD34+ PBSC collection and established a formula capable of predicting CD34+ cell yield. METHODS: We retrospectively evaluated 588 healthy adult donors (median age 29 years, range 18–69 years) at our institution from 2017 to 2022. The predicted minimal number of CD34+ cells was calculated as follows: (peripheral CD34+ cells/µL × adjusted collection efficiency of 30%) × total liters processed. This formula was further modified according to donor and recipient body weight (BW). RESULTS: Median total collection was 8.0 × 10(6) CD34+ cells/kg BW (range 1.0–47.1 × 10(6) cells/kg BW) with 522 donors (89%) collecting ≥5.0 × 10(6) cells/kg of recipient BW. A second leukapheresis (LP) was performed in 49 donors. Need for two LPs was more common in female donors (OR 6.68, 95% CI, 2.62–17.05; p < 0.001), donors with higher age (OR for 10 years difference 1.53, 95% CI, 1.15–2.03, p = 0.003), donors with WBC count <30 × 10(9)/L after 5 days of granulocyte-colony stimulating factor (G-CSF) stimulation (OR, 4.33; 95% CI, 1.59–11.83; p = 0.004), and a donor/recipient weight ratio <1 (OR 6.21, 95% CI, 2.69–14.34; p < 0.001). Predictive factors for optimal LP (i.e., ≥5.0 × 10(6) CD34+ cells/kg of recipient BW) were peripheral blood (PB) CD34+ cell count >50/µL (OR 12.82, range 6.34–25.92, p < 0.001), male donor (OR 2.77, range 1.06–7.23, p = 0.04), and a donor/recipient weight ratio >1 (OR 3.12, range 1.57–6.24, p = 0.001). WBC, platelets, hemoglobin, and age had no significant predictive value. Predicted versus observed number of CD34+ cells/kg BW collected demonstrated a very strong linear correlation (r = 0.925, 95% CI, 0.912–0.936, p < 0.0001). CONCLUSIONS: Of the routinely monitored indicators in PBSC donors, CD34+ cell count in PB is the most important factor in predicting G-CSF-induced PBSC yields. Higher age, female sex, WBC <30 × 10(9)/L, and a donor/recipient weight ratio <1 are useful indicators for identifying suboptimal mobilizers. The modified formula has shown successful and consistent performance in the prediction of key outcome measures including the minimum CD34+ cell collection, determination of the required length of apheresis, and whether a second day of PBSC collection was necessary to achieve the respective collection goal. S. Karger AG 2023-06-30 /pmc/articles/PMC10601602/ /pubmed/37899994 http://dx.doi.org/10.1159/000531236 Text en © 2023 The Author(s). Published by S. Karger AG, Basel https://creativecommons.org/licenses/by-nc/4.0/This article is licensed under the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC) (http://www.karger.com/Services/OpenAccessLicense). Usage and distribution for commercial purposes requires written permission. |
spellingShingle | Research Article Kayser, Sabine Schlenk, Richard F. Steiner, Marcus Klüter, Harald Wuchter, Patrick Predicting Successful Hematopoietic Stem Cell Collection in Healthy Allogeneic Donors |
title | Predicting Successful Hematopoietic Stem Cell Collection in Healthy Allogeneic Donors |
title_full | Predicting Successful Hematopoietic Stem Cell Collection in Healthy Allogeneic Donors |
title_fullStr | Predicting Successful Hematopoietic Stem Cell Collection in Healthy Allogeneic Donors |
title_full_unstemmed | Predicting Successful Hematopoietic Stem Cell Collection in Healthy Allogeneic Donors |
title_short | Predicting Successful Hematopoietic Stem Cell Collection in Healthy Allogeneic Donors |
title_sort | predicting successful hematopoietic stem cell collection in healthy allogeneic donors |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10601602/ https://www.ncbi.nlm.nih.gov/pubmed/37899994 http://dx.doi.org/10.1159/000531236 |
work_keys_str_mv | AT kaysersabine predictingsuccessfulhematopoieticstemcellcollectioninhealthyallogeneicdonors AT schlenkrichardf predictingsuccessfulhematopoieticstemcellcollectioninhealthyallogeneicdonors AT steinermarcus predictingsuccessfulhematopoieticstemcellcollectioninhealthyallogeneicdonors AT kluterharald predictingsuccessfulhematopoieticstemcellcollectioninhealthyallogeneicdonors AT wuchterpatrick predictingsuccessfulhematopoieticstemcellcollectioninhealthyallogeneicdonors |