Cargando…

An oocyte meiotic midbody cap is required for developmental competence in mice

Embryo development depends upon maternally derived materials. Mammalian oocytes undergo extreme asymmetric cytokinesis events, producing one large egg and two small polar bodies (PB). During cytokinesis in somatic cells, the midbody (MB) and subsequent assembly of the midbody remnant (MBR), a signal...

Descripción completa

Detalles Bibliográficos
Autores principales: Schindler, Karen, Jung, Gyu Ik, Londoño-Vásquez, Daniela, Park, Sungjin, Skop, Ahna, Balboula, Ahmed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Journal Experts 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10602078/
https://www.ncbi.nlm.nih.gov/pubmed/37886573
http://dx.doi.org/10.21203/rs.3.rs-3399188/v1
_version_ 1785126319383642112
author Schindler, Karen
Jung, Gyu Ik
Londoño-Vásquez, Daniela
Park, Sungjin
Skop, Ahna
Balboula, Ahmed
author_facet Schindler, Karen
Jung, Gyu Ik
Londoño-Vásquez, Daniela
Park, Sungjin
Skop, Ahna
Balboula, Ahmed
author_sort Schindler, Karen
collection PubMed
description Embryo development depends upon maternally derived materials. Mammalian oocytes undergo extreme asymmetric cytokinesis events, producing one large egg and two small polar bodies (PB). During cytokinesis in somatic cells, the midbody (MB) and subsequent assembly of the midbody remnant (MBR), a signaling organelle containing RNAs, transcription factors and translation machinery, is thought to influence cellular function or fate. The role of the MB and MBR in gametes, in particular, oocytes, remains unclear. Here, we examined the formation and function of meiotic MBs (mMB) and mMB remnants (mMBRs) using mouse oocytes and demonstrate that mMBs have a specialized meiotic mMB cap structure that is orientated toward PBs. We show that that mMBs are translationally active, and that mMB caps are required to retain nascent proteins in eggs. We propose that this specialized mMB cap maintains genetic factors in eggs allowing for full developmental competency.
format Online
Article
Text
id pubmed-10602078
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher American Journal Experts
record_format MEDLINE/PubMed
spelling pubmed-106020782023-10-27 An oocyte meiotic midbody cap is required for developmental competence in mice Schindler, Karen Jung, Gyu Ik Londoño-Vásquez, Daniela Park, Sungjin Skop, Ahna Balboula, Ahmed Res Sq Article Embryo development depends upon maternally derived materials. Mammalian oocytes undergo extreme asymmetric cytokinesis events, producing one large egg and two small polar bodies (PB). During cytokinesis in somatic cells, the midbody (MB) and subsequent assembly of the midbody remnant (MBR), a signaling organelle containing RNAs, transcription factors and translation machinery, is thought to influence cellular function or fate. The role of the MB and MBR in gametes, in particular, oocytes, remains unclear. Here, we examined the formation and function of meiotic MBs (mMB) and mMB remnants (mMBRs) using mouse oocytes and demonstrate that mMBs have a specialized meiotic mMB cap structure that is orientated toward PBs. We show that that mMBs are translationally active, and that mMB caps are required to retain nascent proteins in eggs. We propose that this specialized mMB cap maintains genetic factors in eggs allowing for full developmental competency. American Journal Experts 2023-10-05 /pmc/articles/PMC10602078/ /pubmed/37886573 http://dx.doi.org/10.21203/rs.3.rs-3399188/v1 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Schindler, Karen
Jung, Gyu Ik
Londoño-Vásquez, Daniela
Park, Sungjin
Skop, Ahna
Balboula, Ahmed
An oocyte meiotic midbody cap is required for developmental competence in mice
title An oocyte meiotic midbody cap is required for developmental competence in mice
title_full An oocyte meiotic midbody cap is required for developmental competence in mice
title_fullStr An oocyte meiotic midbody cap is required for developmental competence in mice
title_full_unstemmed An oocyte meiotic midbody cap is required for developmental competence in mice
title_short An oocyte meiotic midbody cap is required for developmental competence in mice
title_sort oocyte meiotic midbody cap is required for developmental competence in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10602078/
https://www.ncbi.nlm.nih.gov/pubmed/37886573
http://dx.doi.org/10.21203/rs.3.rs-3399188/v1
work_keys_str_mv AT schindlerkaren anoocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT junggyuik anoocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT londonovasquezdaniela anoocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT parksungjin anoocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT skopahna anoocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT balboulaahmed anoocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT schindlerkaren oocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT junggyuik oocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT londonovasquezdaniela oocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT parksungjin oocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT skopahna oocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT balboulaahmed oocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice