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An oocyte meiotic midbody cap is required for developmental competence in mice
Embryo development depends upon maternally derived materials. Mammalian oocytes undergo extreme asymmetric cytokinesis events, producing one large egg and two small polar bodies (PB). During cytokinesis in somatic cells, the midbody (MB) and subsequent assembly of the midbody remnant (MBR), a signal...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Journal Experts
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10602078/ https://www.ncbi.nlm.nih.gov/pubmed/37886573 http://dx.doi.org/10.21203/rs.3.rs-3399188/v1 |
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author | Schindler, Karen Jung, Gyu Ik Londoño-Vásquez, Daniela Park, Sungjin Skop, Ahna Balboula, Ahmed |
author_facet | Schindler, Karen Jung, Gyu Ik Londoño-Vásquez, Daniela Park, Sungjin Skop, Ahna Balboula, Ahmed |
author_sort | Schindler, Karen |
collection | PubMed |
description | Embryo development depends upon maternally derived materials. Mammalian oocytes undergo extreme asymmetric cytokinesis events, producing one large egg and two small polar bodies (PB). During cytokinesis in somatic cells, the midbody (MB) and subsequent assembly of the midbody remnant (MBR), a signaling organelle containing RNAs, transcription factors and translation machinery, is thought to influence cellular function or fate. The role of the MB and MBR in gametes, in particular, oocytes, remains unclear. Here, we examined the formation and function of meiotic MBs (mMB) and mMB remnants (mMBRs) using mouse oocytes and demonstrate that mMBs have a specialized meiotic mMB cap structure that is orientated toward PBs. We show that that mMBs are translationally active, and that mMB caps are required to retain nascent proteins in eggs. We propose that this specialized mMB cap maintains genetic factors in eggs allowing for full developmental competency. |
format | Online Article Text |
id | pubmed-10602078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Journal Experts |
record_format | MEDLINE/PubMed |
spelling | pubmed-106020782023-10-27 An oocyte meiotic midbody cap is required for developmental competence in mice Schindler, Karen Jung, Gyu Ik Londoño-Vásquez, Daniela Park, Sungjin Skop, Ahna Balboula, Ahmed Res Sq Article Embryo development depends upon maternally derived materials. Mammalian oocytes undergo extreme asymmetric cytokinesis events, producing one large egg and two small polar bodies (PB). During cytokinesis in somatic cells, the midbody (MB) and subsequent assembly of the midbody remnant (MBR), a signaling organelle containing RNAs, transcription factors and translation machinery, is thought to influence cellular function or fate. The role of the MB and MBR in gametes, in particular, oocytes, remains unclear. Here, we examined the formation and function of meiotic MBs (mMB) and mMB remnants (mMBRs) using mouse oocytes and demonstrate that mMBs have a specialized meiotic mMB cap structure that is orientated toward PBs. We show that that mMBs are translationally active, and that mMB caps are required to retain nascent proteins in eggs. We propose that this specialized mMB cap maintains genetic factors in eggs allowing for full developmental competency. American Journal Experts 2023-10-05 /pmc/articles/PMC10602078/ /pubmed/37886573 http://dx.doi.org/10.21203/rs.3.rs-3399188/v1 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. |
spellingShingle | Article Schindler, Karen Jung, Gyu Ik Londoño-Vásquez, Daniela Park, Sungjin Skop, Ahna Balboula, Ahmed An oocyte meiotic midbody cap is required for developmental competence in mice |
title | An oocyte meiotic midbody cap is required for developmental competence in mice |
title_full | An oocyte meiotic midbody cap is required for developmental competence in mice |
title_fullStr | An oocyte meiotic midbody cap is required for developmental competence in mice |
title_full_unstemmed | An oocyte meiotic midbody cap is required for developmental competence in mice |
title_short | An oocyte meiotic midbody cap is required for developmental competence in mice |
title_sort | oocyte meiotic midbody cap is required for developmental competence in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10602078/ https://www.ncbi.nlm.nih.gov/pubmed/37886573 http://dx.doi.org/10.21203/rs.3.rs-3399188/v1 |
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