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Millimeter-deep micron-resolution vibrational imaging by shortwave infrared photothermal microscopy

Deep-tissue chemical imaging plays a vital role in biological and medical applications. Here, we present a shortwave infrared photothermal (SWIP) microscope for millimeter-deep vibrational imaging with sub-micron lateral resolution and nanoparticle detection sensitivity. By pumping the overtone tran...

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Autores principales: Cheng, Ji-Xin, Ni, Hongli, Yuan, Yuhao, Li, Mingsheng, Zhu, Yifan, Ge, Xiaowei, Yin, Jiaze, Dessai, Chinmayee Prabhu, Wang, Le
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Journal Experts 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10602175/
https://www.ncbi.nlm.nih.gov/pubmed/37886499
http://dx.doi.org/10.21203/rs.3.rs-3449548/v1
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author Cheng, Ji-Xin
Ni, Hongli
Yuan, Yuhao
Li, Mingsheng
Zhu, Yifan
Ge, Xiaowei
Yin, Jiaze
Dessai, Chinmayee Prabhu
Wang, Le
author_facet Cheng, Ji-Xin
Ni, Hongli
Yuan, Yuhao
Li, Mingsheng
Zhu, Yifan
Ge, Xiaowei
Yin, Jiaze
Dessai, Chinmayee Prabhu
Wang, Le
author_sort Cheng, Ji-Xin
collection PubMed
description Deep-tissue chemical imaging plays a vital role in biological and medical applications. Here, we present a shortwave infrared photothermal (SWIP) microscope for millimeter-deep vibrational imaging with sub-micron lateral resolution and nanoparticle detection sensitivity. By pumping the overtone transition of carbon-hydrogen bonds and probing the subsequent photothermal lens with shortwave infrared light, SWIP can obtain chemical contrast from polymer particles located millimeter-deep in a highly scattering phantom. By fast digitization of the optically probed signal, the amplitude of the photothermal signal is shown to be 63 times larger than that of the photoacoustic signal, thus enabling highly sensitive detection of nanoscale objects. SWIP can resolve the intracellular lipids across an intact tumor spheroid and the layered structure in millimeter-thick liver, skin, brain, and breast tissues. Together, SWIP microscopy fills a gap in vibrational imaging with sub-cellular resolution and millimeter-level penetration, which heralds broad potential for life science and clinical applications.
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spelling pubmed-106021752023-10-27 Millimeter-deep micron-resolution vibrational imaging by shortwave infrared photothermal microscopy Cheng, Ji-Xin Ni, Hongli Yuan, Yuhao Li, Mingsheng Zhu, Yifan Ge, Xiaowei Yin, Jiaze Dessai, Chinmayee Prabhu Wang, Le Res Sq Article Deep-tissue chemical imaging plays a vital role in biological and medical applications. Here, we present a shortwave infrared photothermal (SWIP) microscope for millimeter-deep vibrational imaging with sub-micron lateral resolution and nanoparticle detection sensitivity. By pumping the overtone transition of carbon-hydrogen bonds and probing the subsequent photothermal lens with shortwave infrared light, SWIP can obtain chemical contrast from polymer particles located millimeter-deep in a highly scattering phantom. By fast digitization of the optically probed signal, the amplitude of the photothermal signal is shown to be 63 times larger than that of the photoacoustic signal, thus enabling highly sensitive detection of nanoscale objects. SWIP can resolve the intracellular lipids across an intact tumor spheroid and the layered structure in millimeter-thick liver, skin, brain, and breast tissues. Together, SWIP microscopy fills a gap in vibrational imaging with sub-cellular resolution and millimeter-level penetration, which heralds broad potential for life science and clinical applications. American Journal Experts 2023-10-18 /pmc/articles/PMC10602175/ /pubmed/37886499 http://dx.doi.org/10.21203/rs.3.rs-3449548/v1 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Cheng, Ji-Xin
Ni, Hongli
Yuan, Yuhao
Li, Mingsheng
Zhu, Yifan
Ge, Xiaowei
Yin, Jiaze
Dessai, Chinmayee Prabhu
Wang, Le
Millimeter-deep micron-resolution vibrational imaging by shortwave infrared photothermal microscopy
title Millimeter-deep micron-resolution vibrational imaging by shortwave infrared photothermal microscopy
title_full Millimeter-deep micron-resolution vibrational imaging by shortwave infrared photothermal microscopy
title_fullStr Millimeter-deep micron-resolution vibrational imaging by shortwave infrared photothermal microscopy
title_full_unstemmed Millimeter-deep micron-resolution vibrational imaging by shortwave infrared photothermal microscopy
title_short Millimeter-deep micron-resolution vibrational imaging by shortwave infrared photothermal microscopy
title_sort millimeter-deep micron-resolution vibrational imaging by shortwave infrared photothermal microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10602175/
https://www.ncbi.nlm.nih.gov/pubmed/37886499
http://dx.doi.org/10.21203/rs.3.rs-3449548/v1
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