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Metagenomics analysis reveals presence of the Merida-like virus in Georgia

Arbovirus surveillance is fundamental for the discovery of novel viruses and prevention of febrile vector-borne illnesses. Vector-borne pathogens can rapidly expand and adapt in new geographic and environmental conditions. In this study, metagenomic surveillance was conducted to identify novel virus...

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Autores principales: Potter-Birriel, Jennifer M., Pollio, Adam R., Knott, Brian D., Chunashvili, Tamar, Fung, Christian K., Conte, Matthew A., Reinbold-Wasson, Drew D., Hang, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10602647/
https://www.ncbi.nlm.nih.gov/pubmed/37901812
http://dx.doi.org/10.3389/fmicb.2023.1258810
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author Potter-Birriel, Jennifer M.
Pollio, Adam R.
Knott, Brian D.
Chunashvili, Tamar
Fung, Christian K.
Conte, Matthew A.
Reinbold-Wasson, Drew D.
Hang, Jun
author_facet Potter-Birriel, Jennifer M.
Pollio, Adam R.
Knott, Brian D.
Chunashvili, Tamar
Fung, Christian K.
Conte, Matthew A.
Reinbold-Wasson, Drew D.
Hang, Jun
author_sort Potter-Birriel, Jennifer M.
collection PubMed
description Arbovirus surveillance is fundamental for the discovery of novel viruses and prevention of febrile vector-borne illnesses. Vector-borne pathogens can rapidly expand and adapt in new geographic and environmental conditions. In this study, metagenomic surveillance was conducted to identify novel viruses in the Country of Georgia. A total of 521 mosquitoes were captured near a military training facility and pooled from species Culex pipiens (Linnaeus) (87%) and Aedes albopictus (Skuse) (13%). We decided to further analyze the Culex pipiens mosquitoes, due to the more extensive number of samples collected. Our approach was to utilize an unbiased total RNA-seq for pathogen discovery in order to explore the mosquito virome. The viral reads from this analysis were mostly aligned to Insect-specific viruses from two main families, the Iflaviridae; a positive-stranded RNA virus and the Rhabdoviridae; a negative- and single-stranded RNA virus. Our pathogen discovery analysis revealed viral reads aligning to the Merida-like virus Turkey (MERDLVT) strain among the Rhabdoviridae. To further validate this result, we conducted a BLAST sequence comparison analysis of our samples with the MERDLVT strain. Our positive samples aligned to the MERDLVT strain with 96–100% sequence identity and 99.7–100% sequence coverage. A bootstrapped maximum-likelihood phylogenetic tree was used to evaluate the evolutionary relationships among these positive pooled specimens with the (MERDLVT) strain. The Georgia samples clustered most closely with two strains from Turkey, the Merida-like virus KE-2017a isolate 139-1-21 and the Merida-like virus Turkey isolate P431. Collectively, these results show the presence of the MERDLVT strain in Georgia.
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spelling pubmed-106026472023-10-27 Metagenomics analysis reveals presence of the Merida-like virus in Georgia Potter-Birriel, Jennifer M. Pollio, Adam R. Knott, Brian D. Chunashvili, Tamar Fung, Christian K. Conte, Matthew A. Reinbold-Wasson, Drew D. Hang, Jun Front Microbiol Microbiology Arbovirus surveillance is fundamental for the discovery of novel viruses and prevention of febrile vector-borne illnesses. Vector-borne pathogens can rapidly expand and adapt in new geographic and environmental conditions. In this study, metagenomic surveillance was conducted to identify novel viruses in the Country of Georgia. A total of 521 mosquitoes were captured near a military training facility and pooled from species Culex pipiens (Linnaeus) (87%) and Aedes albopictus (Skuse) (13%). We decided to further analyze the Culex pipiens mosquitoes, due to the more extensive number of samples collected. Our approach was to utilize an unbiased total RNA-seq for pathogen discovery in order to explore the mosquito virome. The viral reads from this analysis were mostly aligned to Insect-specific viruses from two main families, the Iflaviridae; a positive-stranded RNA virus and the Rhabdoviridae; a negative- and single-stranded RNA virus. Our pathogen discovery analysis revealed viral reads aligning to the Merida-like virus Turkey (MERDLVT) strain among the Rhabdoviridae. To further validate this result, we conducted a BLAST sequence comparison analysis of our samples with the MERDLVT strain. Our positive samples aligned to the MERDLVT strain with 96–100% sequence identity and 99.7–100% sequence coverage. A bootstrapped maximum-likelihood phylogenetic tree was used to evaluate the evolutionary relationships among these positive pooled specimens with the (MERDLVT) strain. The Georgia samples clustered most closely with two strains from Turkey, the Merida-like virus KE-2017a isolate 139-1-21 and the Merida-like virus Turkey isolate P431. Collectively, these results show the presence of the MERDLVT strain in Georgia. Frontiers Media S.A. 2023-10-12 /pmc/articles/PMC10602647/ /pubmed/37901812 http://dx.doi.org/10.3389/fmicb.2023.1258810 Text en Copyright © 2023 Potter-Birriel, Pollio, Knott, Chunashvili, Fung, Conte, Reinbold-Wasson and Hang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Potter-Birriel, Jennifer M.
Pollio, Adam R.
Knott, Brian D.
Chunashvili, Tamar
Fung, Christian K.
Conte, Matthew A.
Reinbold-Wasson, Drew D.
Hang, Jun
Metagenomics analysis reveals presence of the Merida-like virus in Georgia
title Metagenomics analysis reveals presence of the Merida-like virus in Georgia
title_full Metagenomics analysis reveals presence of the Merida-like virus in Georgia
title_fullStr Metagenomics analysis reveals presence of the Merida-like virus in Georgia
title_full_unstemmed Metagenomics analysis reveals presence of the Merida-like virus in Georgia
title_short Metagenomics analysis reveals presence of the Merida-like virus in Georgia
title_sort metagenomics analysis reveals presence of the merida-like virus in georgia
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10602647/
https://www.ncbi.nlm.nih.gov/pubmed/37901812
http://dx.doi.org/10.3389/fmicb.2023.1258810
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