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Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, China

Piroplasmosis is a zoonotic disease mainly caused by the Babesia and Theileria parasites. Piroplasmosis is often a subclinical infection in dogs and cats that is difficult to detect and is often suspected when clinical signs such as anemia are present. It has been reported to be prevalent in China....

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Autores principales: Wu, Shengchun, Meng, Jiao, Yu, Fuxun, Zhou, Caomin, Yang, Bin, Chen, Xingxing, Yang, Guanghong, Sun, Yi, Cao, Wuchun, Jiang, Jiafu, Wu, Jiahong, Zhan, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10602747/
https://www.ncbi.nlm.nih.gov/pubmed/37901826
http://dx.doi.org/10.3389/fmicb.2023.1266583
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author Wu, Shengchun
Meng, Jiao
Yu, Fuxun
Zhou, Caomin
Yang, Bin
Chen, Xingxing
Yang, Guanghong
Sun, Yi
Cao, Wuchun
Jiang, Jiafu
Wu, Jiahong
Zhan, Lin
author_facet Wu, Shengchun
Meng, Jiao
Yu, Fuxun
Zhou, Caomin
Yang, Bin
Chen, Xingxing
Yang, Guanghong
Sun, Yi
Cao, Wuchun
Jiang, Jiafu
Wu, Jiahong
Zhan, Lin
author_sort Wu, Shengchun
collection PubMed
description Piroplasmosis is a zoonotic disease mainly caused by the Babesia and Theileria parasites. Piroplasmosis is often a subclinical infection in dogs and cats that is difficult to detect and is often suspected when clinical signs such as anemia are present. It has been reported to be prevalent in China. However, molecular evidence of the disease has not been reported in pet dogs and cats in Guiyang. In this study, we collected 307 anticoagulated blood samples from an animal hospital in the Wudang District of Guiyang during the period March 2021 to November 2021 and extracted DNA from the samples. The 18S rDNA gene was amplified using PCR, and the positive amplification product was sequenced. The sequences were then analyzed for homology and phylogeny. Of the 307 samples collected, 164 were feline and 143 were canine, with a total of 23 amplifying a target band of approximately 400 bp. The percentage of positives of piroplasms infection in pet cats was 4.27% (7/164), with the pathogens being T. uilenbergi (3) and T. luwenshuni (4). One Colpodella sp. and two undetermined species were also detected in the cat samples. The percentage of positives of piroplasms infection in pet dogs was 7.69% (11/143), with the pathogen being T. uilenbergi (11). One Colpodella sp. was also detected in the dog samples. The results confirmed that T. uilenbergi and T. luwenshuni are prevalent in pet cats and dogs in this area. In addition, the study found a rare zoonotic pathogen, Colpodella sp., in cats and dogs. Therefore, this study is expected to serve as a valuable reference for decision-making regarding animal health management and public health work.
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spelling pubmed-106027472023-10-27 Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, China Wu, Shengchun Meng, Jiao Yu, Fuxun Zhou, Caomin Yang, Bin Chen, Xingxing Yang, Guanghong Sun, Yi Cao, Wuchun Jiang, Jiafu Wu, Jiahong Zhan, Lin Front Microbiol Microbiology Piroplasmosis is a zoonotic disease mainly caused by the Babesia and Theileria parasites. Piroplasmosis is often a subclinical infection in dogs and cats that is difficult to detect and is often suspected when clinical signs such as anemia are present. It has been reported to be prevalent in China. However, molecular evidence of the disease has not been reported in pet dogs and cats in Guiyang. In this study, we collected 307 anticoagulated blood samples from an animal hospital in the Wudang District of Guiyang during the period March 2021 to November 2021 and extracted DNA from the samples. The 18S rDNA gene was amplified using PCR, and the positive amplification product was sequenced. The sequences were then analyzed for homology and phylogeny. Of the 307 samples collected, 164 were feline and 143 were canine, with a total of 23 amplifying a target band of approximately 400 bp. The percentage of positives of piroplasms infection in pet cats was 4.27% (7/164), with the pathogens being T. uilenbergi (3) and T. luwenshuni (4). One Colpodella sp. and two undetermined species were also detected in the cat samples. The percentage of positives of piroplasms infection in pet dogs was 7.69% (11/143), with the pathogen being T. uilenbergi (11). One Colpodella sp. was also detected in the dog samples. The results confirmed that T. uilenbergi and T. luwenshuni are prevalent in pet cats and dogs in this area. In addition, the study found a rare zoonotic pathogen, Colpodella sp., in cats and dogs. Therefore, this study is expected to serve as a valuable reference for decision-making regarding animal health management and public health work. Frontiers Media S.A. 2023-10-12 /pmc/articles/PMC10602747/ /pubmed/37901826 http://dx.doi.org/10.3389/fmicb.2023.1266583 Text en Copyright © 2023 Wu, Meng, Yu, Zhou, Yang, Chen, Yang, Sun, Cao, Jiang, Wu and Zhan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wu, Shengchun
Meng, Jiao
Yu, Fuxun
Zhou, Caomin
Yang, Bin
Chen, Xingxing
Yang, Guanghong
Sun, Yi
Cao, Wuchun
Jiang, Jiafu
Wu, Jiahong
Zhan, Lin
Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, China
title Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, China
title_full Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, China
title_fullStr Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, China
title_full_unstemmed Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, China
title_short Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, China
title_sort molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in guiyang, china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10602747/
https://www.ncbi.nlm.nih.gov/pubmed/37901826
http://dx.doi.org/10.3389/fmicb.2023.1266583
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