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Global analyses and potential effects of extracellular vesicles on the establishment of conceptus implantation during the peri-implantation period

Intrauterine extracellular vesicles (EVs) are involved in establishing proper conceptus-endometrial communication, which is essential for conceptus implantation and subsequent successful placentation. Despite several studies on intrauterine EVs, the composition and quantitative changes in conceptus...

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Autores principales: NAKAMURA, Keigo, KUSAMA, Kazuya, HORI, Masatoshi, IMAKAWA, Kazuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10602766/
https://www.ncbi.nlm.nih.gov/pubmed/37495510
http://dx.doi.org/10.1262/jrd.2023-044
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author NAKAMURA, Keigo
KUSAMA, Kazuya
HORI, Masatoshi
IMAKAWA, Kazuhiko
author_facet NAKAMURA, Keigo
KUSAMA, Kazuya
HORI, Masatoshi
IMAKAWA, Kazuhiko
author_sort NAKAMURA, Keigo
collection PubMed
description Intrauterine extracellular vesicles (EVs) are involved in establishing proper conceptus-endometrial communication, which is essential for conceptus implantation and subsequent successful placentation. Despite several studies on intrauterine EVs, the composition and quantitative changes in conceptus and endometrial EVs, as well as the effects of intrauterine EVs on endometrial epithelial cells (EECs) during the peri-implantation period, have not been well characterized. To elucidate global changes in proteins in EVs extracted from uterine flushings (UFs) during the pre-implantation (P17), just-implantation (P20), and post-implantation (P22) periods, the datasets of the proteome iTRAQ analysis were compared among P17, P20, and P22 EVs. These analyses revealed that the composition and function of proteins in the EVs changed dramatically during peri-implantation in cattle. Notably, intrauterine P17 EVs affected the high expression of “Developmental Biology” and “morphogenesis of an endothelium” compared with those in P20 and P22 EVs. Furthermore, P20 EVs had the functions of the high expression of “mitochondrial calcium ion homeostasis” and “Viral mRNA Translation” compared with those in P17 EVs. Transcripts extracted from EECs treated with P17, P20, or P22 EVs were subjected to RNA-seq analysis. These analyses identified 60 transcripts in EECs commonly induced by intrauterine EVs recovered from P17, P20, and P22, a large number of which were associated with “type I interferon signaling pathway”. Collectively, these findings reveal the presence and multiple functions of EVs that are potentially implicated in facilitating conceptus implantation into the uterine epithelium during the peri-implantation period.
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spelling pubmed-106027662023-10-28 Global analyses and potential effects of extracellular vesicles on the establishment of conceptus implantation during the peri-implantation period NAKAMURA, Keigo KUSAMA, Kazuya HORI, Masatoshi IMAKAWA, Kazuhiko J Reprod Dev Original Article Intrauterine extracellular vesicles (EVs) are involved in establishing proper conceptus-endometrial communication, which is essential for conceptus implantation and subsequent successful placentation. Despite several studies on intrauterine EVs, the composition and quantitative changes in conceptus and endometrial EVs, as well as the effects of intrauterine EVs on endometrial epithelial cells (EECs) during the peri-implantation period, have not been well characterized. To elucidate global changes in proteins in EVs extracted from uterine flushings (UFs) during the pre-implantation (P17), just-implantation (P20), and post-implantation (P22) periods, the datasets of the proteome iTRAQ analysis were compared among P17, P20, and P22 EVs. These analyses revealed that the composition and function of proteins in the EVs changed dramatically during peri-implantation in cattle. Notably, intrauterine P17 EVs affected the high expression of “Developmental Biology” and “morphogenesis of an endothelium” compared with those in P20 and P22 EVs. Furthermore, P20 EVs had the functions of the high expression of “mitochondrial calcium ion homeostasis” and “Viral mRNA Translation” compared with those in P17 EVs. Transcripts extracted from EECs treated with P17, P20, or P22 EVs were subjected to RNA-seq analysis. These analyses identified 60 transcripts in EECs commonly induced by intrauterine EVs recovered from P17, P20, and P22, a large number of which were associated with “type I interferon signaling pathway”. Collectively, these findings reveal the presence and multiple functions of EVs that are potentially implicated in facilitating conceptus implantation into the uterine epithelium during the peri-implantation period. The Society for Reproduction and Development 2023-07-25 2023-10 /pmc/articles/PMC10602766/ /pubmed/37495510 http://dx.doi.org/10.1262/jrd.2023-044 Text en ©2023 Society for Reproduction and Development https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original Article
NAKAMURA, Keigo
KUSAMA, Kazuya
HORI, Masatoshi
IMAKAWA, Kazuhiko
Global analyses and potential effects of extracellular vesicles on the establishment of conceptus implantation during the peri-implantation period
title Global analyses and potential effects of extracellular vesicles on the establishment of conceptus implantation during the peri-implantation period
title_full Global analyses and potential effects of extracellular vesicles on the establishment of conceptus implantation during the peri-implantation period
title_fullStr Global analyses and potential effects of extracellular vesicles on the establishment of conceptus implantation during the peri-implantation period
title_full_unstemmed Global analyses and potential effects of extracellular vesicles on the establishment of conceptus implantation during the peri-implantation period
title_short Global analyses and potential effects of extracellular vesicles on the establishment of conceptus implantation during the peri-implantation period
title_sort global analyses and potential effects of extracellular vesicles on the establishment of conceptus implantation during the peri-implantation period
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10602766/
https://www.ncbi.nlm.nih.gov/pubmed/37495510
http://dx.doi.org/10.1262/jrd.2023-044
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