Cargando…

Synergistic effects of electroactive antibacterial material and electrical stimulation in enhancing skin tissue regeneration: A next‐generation dermal wound dressing

OBJECTIVE: We aimed to develop an electroactive antibacterial material for the treatment of skin wound diseases. METHOD: To this aim, we modified chitosan (CS), a biocompatible polymer, by coupling it with graphene (rGO) and an antimicrobial polypeptide DOPA‐PonG1. The material's effect on skin...

Descripción completa

Detalles Bibliográficos
Autores principales: Zang, Xi‐Liang, Gao, Fei, Zhang, Zhao, Shen, Lin‐Hua, Pan, Yue‐Hai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10603310/
https://www.ncbi.nlm.nih.gov/pubmed/38009021
http://dx.doi.org/10.1111/srt.13465
_version_ 1785126578663981056
author Zang, Xi‐Liang
Gao, Fei
Zhang, Zhao
Shen, Lin‐Hua
Pan, Yue‐Hai
author_facet Zang, Xi‐Liang
Gao, Fei
Zhang, Zhao
Shen, Lin‐Hua
Pan, Yue‐Hai
author_sort Zang, Xi‐Liang
collection PubMed
description OBJECTIVE: We aimed to develop an electroactive antibacterial material for the treatment of skin wound diseases. METHOD: To this aim, we modified chitosan (CS), a biocompatible polymer, by coupling it with graphene (rGO) and an antimicrobial polypeptide DOPA‐PonG1. The material's effect on skin injury healing was studied in combination with external electrical stimulation (EEM). The structure, surface composition, and hydrophilicity of the modified CS materials were evaluated using scanning electron microscopy (SEM), Fourier‐transform infrared spectroscopy (FTIR), and contact angle measurements. We studied NIH3T3 cells cultured with modified materials and subjected to EEM to assess viability, adhesion, and tissue repair‐related gene expression. RESULTS: SEM data demonstrated that rGO was distributed uniformly on the surface of the CS material, increasing surface roughness, and antimicrobial peptides had minimal impact on surface morphology. FTIR confirmed the uniform distribution of rGO and antibacterial peptides on the material surface. Both rGO and DOPA‐PonG1 enhanced the hydrophilicity of CS materials, with rGO also improving tensile strength. The dual modification of CS with rGO and DOPA‐PonG1 synergistically increased antibacterial efficacy. Cellular events and gene expression relevant to tissue repair process were enhanced by these modifications. Furthermore, EEM accelerated epidermal regeneration more than the material alone. In a rat skin wound model, DOPA‐PonG1@CS/rGO dressing combined with electrical stimulation exhibited accelerated healing of skin defect. CONCLUSION: Overall, our results demonstrate that CS materials modified with rGO and DOPA‐PonG1 have increased hydrophilicity, antibacterial characteristics, and tissue regeneration capacities. This modified material in conjunction with EEM hold promise for the clinical management for dermal wounds.
format Online
Article
Text
id pubmed-10603310
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-106033102023-10-28 Synergistic effects of electroactive antibacterial material and electrical stimulation in enhancing skin tissue regeneration: A next‐generation dermal wound dressing Zang, Xi‐Liang Gao, Fei Zhang, Zhao Shen, Lin‐Hua Pan, Yue‐Hai Skin Res Technol Original Articles OBJECTIVE: We aimed to develop an electroactive antibacterial material for the treatment of skin wound diseases. METHOD: To this aim, we modified chitosan (CS), a biocompatible polymer, by coupling it with graphene (rGO) and an antimicrobial polypeptide DOPA‐PonG1. The material's effect on skin injury healing was studied in combination with external electrical stimulation (EEM). The structure, surface composition, and hydrophilicity of the modified CS materials were evaluated using scanning electron microscopy (SEM), Fourier‐transform infrared spectroscopy (FTIR), and contact angle measurements. We studied NIH3T3 cells cultured with modified materials and subjected to EEM to assess viability, adhesion, and tissue repair‐related gene expression. RESULTS: SEM data demonstrated that rGO was distributed uniformly on the surface of the CS material, increasing surface roughness, and antimicrobial peptides had minimal impact on surface morphology. FTIR confirmed the uniform distribution of rGO and antibacterial peptides on the material surface. Both rGO and DOPA‐PonG1 enhanced the hydrophilicity of CS materials, with rGO also improving tensile strength. The dual modification of CS with rGO and DOPA‐PonG1 synergistically increased antibacterial efficacy. Cellular events and gene expression relevant to tissue repair process were enhanced by these modifications. Furthermore, EEM accelerated epidermal regeneration more than the material alone. In a rat skin wound model, DOPA‐PonG1@CS/rGO dressing combined with electrical stimulation exhibited accelerated healing of skin defect. CONCLUSION: Overall, our results demonstrate that CS materials modified with rGO and DOPA‐PonG1 have increased hydrophilicity, antibacterial characteristics, and tissue regeneration capacities. This modified material in conjunction with EEM hold promise for the clinical management for dermal wounds. John Wiley and Sons Inc. 2023-10-26 /pmc/articles/PMC10603310/ /pubmed/38009021 http://dx.doi.org/10.1111/srt.13465 Text en © 2023 The Authors. Skin Research and Technology published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Zang, Xi‐Liang
Gao, Fei
Zhang, Zhao
Shen, Lin‐Hua
Pan, Yue‐Hai
Synergistic effects of electroactive antibacterial material and electrical stimulation in enhancing skin tissue regeneration: A next‐generation dermal wound dressing
title Synergistic effects of electroactive antibacterial material and electrical stimulation in enhancing skin tissue regeneration: A next‐generation dermal wound dressing
title_full Synergistic effects of electroactive antibacterial material and electrical stimulation in enhancing skin tissue regeneration: A next‐generation dermal wound dressing
title_fullStr Synergistic effects of electroactive antibacterial material and electrical stimulation in enhancing skin tissue regeneration: A next‐generation dermal wound dressing
title_full_unstemmed Synergistic effects of electroactive antibacterial material and electrical stimulation in enhancing skin tissue regeneration: A next‐generation dermal wound dressing
title_short Synergistic effects of electroactive antibacterial material and electrical stimulation in enhancing skin tissue regeneration: A next‐generation dermal wound dressing
title_sort synergistic effects of electroactive antibacterial material and electrical stimulation in enhancing skin tissue regeneration: a next‐generation dermal wound dressing
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10603310/
https://www.ncbi.nlm.nih.gov/pubmed/38009021
http://dx.doi.org/10.1111/srt.13465
work_keys_str_mv AT zangxiliang synergisticeffectsofelectroactiveantibacterialmaterialandelectricalstimulationinenhancingskintissueregenerationanextgenerationdermalwounddressing
AT gaofei synergisticeffectsofelectroactiveantibacterialmaterialandelectricalstimulationinenhancingskintissueregenerationanextgenerationdermalwounddressing
AT zhangzhao synergisticeffectsofelectroactiveantibacterialmaterialandelectricalstimulationinenhancingskintissueregenerationanextgenerationdermalwounddressing
AT shenlinhua synergisticeffectsofelectroactiveantibacterialmaterialandelectricalstimulationinenhancingskintissueregenerationanextgenerationdermalwounddressing
AT panyuehai synergisticeffectsofelectroactiveantibacterialmaterialandelectricalstimulationinenhancingskintissueregenerationanextgenerationdermalwounddressing