Direct Comparison between Förster Resonance Energy Transfer and Light-Induced Triplet–Triplet Electron Resonance Spectroscopy

[Image: see text] To carry out reliable and comprehensive structural investigations, the exploitation of different complementary techniques is required. Here, we report that dual triplet-spin/fluorescent labels enable the first parallel distance measurements by electron spin resonance (ESR) and Förs...

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Autores principales: Bertran, Arnau, Morbiato, Laura, Sawyer, Jack, Dalla Torre, Chiara, Heyes, Derren J., Hay, Sam, Timmel, Christiane R., Di Valentin, Marilena, De Zotti, Marta, Bowen, Alice M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10603778/
https://www.ncbi.nlm.nih.gov/pubmed/37839071
http://dx.doi.org/10.1021/jacs.3c04685
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author Bertran, Arnau
Morbiato, Laura
Sawyer, Jack
Dalla Torre, Chiara
Heyes, Derren J.
Hay, Sam
Timmel, Christiane R.
Di Valentin, Marilena
De Zotti, Marta
Bowen, Alice M.
author_facet Bertran, Arnau
Morbiato, Laura
Sawyer, Jack
Dalla Torre, Chiara
Heyes, Derren J.
Hay, Sam
Timmel, Christiane R.
Di Valentin, Marilena
De Zotti, Marta
Bowen, Alice M.
author_sort Bertran, Arnau
collection PubMed
description [Image: see text] To carry out reliable and comprehensive structural investigations, the exploitation of different complementary techniques is required. Here, we report that dual triplet-spin/fluorescent labels enable the first parallel distance measurements by electron spin resonance (ESR) and Förster resonance energy transfer (FRET) on exactly the same molecules with orthogonal chromophores, allowing for direct comparison. An improved light-induced triplet–triplet electron resonance method with 2-color excitation is used, improving the signal-to-noise ratio of the data and yielding a distance distribution that provides greater insight than the single distance resulting from FRET.
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spelling pubmed-106037782023-10-28 Direct Comparison between Förster Resonance Energy Transfer and Light-Induced Triplet–Triplet Electron Resonance Spectroscopy Bertran, Arnau Morbiato, Laura Sawyer, Jack Dalla Torre, Chiara Heyes, Derren J. Hay, Sam Timmel, Christiane R. Di Valentin, Marilena De Zotti, Marta Bowen, Alice M. J Am Chem Soc [Image: see text] To carry out reliable and comprehensive structural investigations, the exploitation of different complementary techniques is required. Here, we report that dual triplet-spin/fluorescent labels enable the first parallel distance measurements by electron spin resonance (ESR) and Förster resonance energy transfer (FRET) on exactly the same molecules with orthogonal chromophores, allowing for direct comparison. An improved light-induced triplet–triplet electron resonance method with 2-color excitation is used, improving the signal-to-noise ratio of the data and yielding a distance distribution that provides greater insight than the single distance resulting from FRET. American Chemical Society 2023-10-15 /pmc/articles/PMC10603778/ /pubmed/37839071 http://dx.doi.org/10.1021/jacs.3c04685 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Bertran, Arnau
Morbiato, Laura
Sawyer, Jack
Dalla Torre, Chiara
Heyes, Derren J.
Hay, Sam
Timmel, Christiane R.
Di Valentin, Marilena
De Zotti, Marta
Bowen, Alice M.
Direct Comparison between Förster Resonance Energy Transfer and Light-Induced Triplet–Triplet Electron Resonance Spectroscopy
title Direct Comparison between Förster Resonance Energy Transfer and Light-Induced Triplet–Triplet Electron Resonance Spectroscopy
title_full Direct Comparison between Förster Resonance Energy Transfer and Light-Induced Triplet–Triplet Electron Resonance Spectroscopy
title_fullStr Direct Comparison between Förster Resonance Energy Transfer and Light-Induced Triplet–Triplet Electron Resonance Spectroscopy
title_full_unstemmed Direct Comparison between Förster Resonance Energy Transfer and Light-Induced Triplet–Triplet Electron Resonance Spectroscopy
title_short Direct Comparison between Förster Resonance Energy Transfer and Light-Induced Triplet–Triplet Electron Resonance Spectroscopy
title_sort direct comparison between förster resonance energy transfer and light-induced triplet–triplet electron resonance spectroscopy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10603778/
https://www.ncbi.nlm.nih.gov/pubmed/37839071
http://dx.doi.org/10.1021/jacs.3c04685
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