Cargando…
Integrated Transcriptomic and Proteomic Analyses of Low-Nitrogen-Stress Tolerance and Function Analysis of ZmGST42 Gene in Maize
Maize (Zea mays L.) is one of the major staple crops providing human food, animal feed, and raw material support for biofuel production. For its growth and development, maize requires essential macronutrients. In particular, nitrogen (N) plays an important role in determining the final yield and qua...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10603844/ https://www.ncbi.nlm.nih.gov/pubmed/37891910 http://dx.doi.org/10.3390/antiox12101831 |
_version_ | 1785126692805672960 |
---|---|
author | Li, Jiao Zenda, Tinashe Liu, Songtao Dong, Anyi Wang, Yafei Liu, Xinyue Wang, Nan Duan, Huijun |
author_facet | Li, Jiao Zenda, Tinashe Liu, Songtao Dong, Anyi Wang, Yafei Liu, Xinyue Wang, Nan Duan, Huijun |
author_sort | Li, Jiao |
collection | PubMed |
description | Maize (Zea mays L.) is one of the major staple crops providing human food, animal feed, and raw material support for biofuel production. For its growth and development, maize requires essential macronutrients. In particular, nitrogen (N) plays an important role in determining the final yield and quality of a maize crop. However, the excessive application of N fertilizer is causing serious pollution of land area and water bodies. Therefore, cultivating high-yield and low-N-tolerant maize varieties is crucial for minimizing the nitrate pollution of land and water bodies. Here, based on the analysis of the maize leaf transcriptome and proteome at the grain filling stage, we identified 3957 differentially expressed genes (DEGs) and 329 differentially abundant proteins (DAPs) from the two maize hybrids contrasting in N stress tolerance (low-N-tolerant XY335 and low-N-sensitive HN138) and screened four sets of low-N-responsive genes and proteins through Venn diagram analysis. We identified 761 DEGs (253 up- and 508 down-regulated) specific to XY335, whereas 259 DEGs (198 up- and 61 down-regulated) were specific to HN138, and 59 DEGs (41 up- and 18 down-regulated) were shared between the two cultivars under low-N-stress conditions. Meanwhile, among the low-N-responsive DAPs, thirty were unique to XY335, thirty were specific to HN138, and three DAPs were shared between the two cultivars under low-N treatment. Key among those genes/proteins were leucine-rich repeat protein, DEAD-box ATP-dependent RNA helicase family proteins, copper transport protein, and photosynthesis-related proteins. These genes/proteins were involved in the MAPK signaling pathway, regulating membrane lipid peroxidation, and photosynthesis. Our results may suggest that XY335 better tolerates low-N stress than HN138, possibly through robust low-N-stress sensing and signaling, amplified protein phosphorylation and stress response, and increased photosynthesis efficiency, as well as the down-regulation of ‘lavish’ or redundant proteins to minimize N demand. Additionally, we screened glutathione transferase 42 (ZmGST42) and performed physiological and biochemical characterizations of the wild-type (B73) and gst42 mutant at the seedling stage. Resultantly, the wild-type exhibited stronger tolerance to low N than the mutant line. Our findings provide a better understanding of the molecular mechanisms underlying low-N tolerance during the maize grain filling stage and reveal key candidate genes for low-N-tolerance breeding in maize. |
format | Online Article Text |
id | pubmed-10603844 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106038442023-10-28 Integrated Transcriptomic and Proteomic Analyses of Low-Nitrogen-Stress Tolerance and Function Analysis of ZmGST42 Gene in Maize Li, Jiao Zenda, Tinashe Liu, Songtao Dong, Anyi Wang, Yafei Liu, Xinyue Wang, Nan Duan, Huijun Antioxidants (Basel) Article Maize (Zea mays L.) is one of the major staple crops providing human food, animal feed, and raw material support for biofuel production. For its growth and development, maize requires essential macronutrients. In particular, nitrogen (N) plays an important role in determining the final yield and quality of a maize crop. However, the excessive application of N fertilizer is causing serious pollution of land area and water bodies. Therefore, cultivating high-yield and low-N-tolerant maize varieties is crucial for minimizing the nitrate pollution of land and water bodies. Here, based on the analysis of the maize leaf transcriptome and proteome at the grain filling stage, we identified 3957 differentially expressed genes (DEGs) and 329 differentially abundant proteins (DAPs) from the two maize hybrids contrasting in N stress tolerance (low-N-tolerant XY335 and low-N-sensitive HN138) and screened four sets of low-N-responsive genes and proteins through Venn diagram analysis. We identified 761 DEGs (253 up- and 508 down-regulated) specific to XY335, whereas 259 DEGs (198 up- and 61 down-regulated) were specific to HN138, and 59 DEGs (41 up- and 18 down-regulated) were shared between the two cultivars under low-N-stress conditions. Meanwhile, among the low-N-responsive DAPs, thirty were unique to XY335, thirty were specific to HN138, and three DAPs were shared between the two cultivars under low-N treatment. Key among those genes/proteins were leucine-rich repeat protein, DEAD-box ATP-dependent RNA helicase family proteins, copper transport protein, and photosynthesis-related proteins. These genes/proteins were involved in the MAPK signaling pathway, regulating membrane lipid peroxidation, and photosynthesis. Our results may suggest that XY335 better tolerates low-N stress than HN138, possibly through robust low-N-stress sensing and signaling, amplified protein phosphorylation and stress response, and increased photosynthesis efficiency, as well as the down-regulation of ‘lavish’ or redundant proteins to minimize N demand. Additionally, we screened glutathione transferase 42 (ZmGST42) and performed physiological and biochemical characterizations of the wild-type (B73) and gst42 mutant at the seedling stage. Resultantly, the wild-type exhibited stronger tolerance to low N than the mutant line. Our findings provide a better understanding of the molecular mechanisms underlying low-N tolerance during the maize grain filling stage and reveal key candidate genes for low-N-tolerance breeding in maize. MDPI 2023-10-05 /pmc/articles/PMC10603844/ /pubmed/37891910 http://dx.doi.org/10.3390/antiox12101831 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Jiao Zenda, Tinashe Liu, Songtao Dong, Anyi Wang, Yafei Liu, Xinyue Wang, Nan Duan, Huijun Integrated Transcriptomic and Proteomic Analyses of Low-Nitrogen-Stress Tolerance and Function Analysis of ZmGST42 Gene in Maize |
title | Integrated Transcriptomic and Proteomic Analyses of Low-Nitrogen-Stress Tolerance and Function Analysis of ZmGST42 Gene in Maize |
title_full | Integrated Transcriptomic and Proteomic Analyses of Low-Nitrogen-Stress Tolerance and Function Analysis of ZmGST42 Gene in Maize |
title_fullStr | Integrated Transcriptomic and Proteomic Analyses of Low-Nitrogen-Stress Tolerance and Function Analysis of ZmGST42 Gene in Maize |
title_full_unstemmed | Integrated Transcriptomic and Proteomic Analyses of Low-Nitrogen-Stress Tolerance and Function Analysis of ZmGST42 Gene in Maize |
title_short | Integrated Transcriptomic and Proteomic Analyses of Low-Nitrogen-Stress Tolerance and Function Analysis of ZmGST42 Gene in Maize |
title_sort | integrated transcriptomic and proteomic analyses of low-nitrogen-stress tolerance and function analysis of zmgst42 gene in maize |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10603844/ https://www.ncbi.nlm.nih.gov/pubmed/37891910 http://dx.doi.org/10.3390/antiox12101831 |
work_keys_str_mv | AT lijiao integratedtranscriptomicandproteomicanalysesoflownitrogenstresstoleranceandfunctionanalysisofzmgst42geneinmaize AT zendatinashe integratedtranscriptomicandproteomicanalysesoflownitrogenstresstoleranceandfunctionanalysisofzmgst42geneinmaize AT liusongtao integratedtranscriptomicandproteomicanalysesoflownitrogenstresstoleranceandfunctionanalysisofzmgst42geneinmaize AT donganyi integratedtranscriptomicandproteomicanalysesoflownitrogenstresstoleranceandfunctionanalysisofzmgst42geneinmaize AT wangyafei integratedtranscriptomicandproteomicanalysesoflownitrogenstresstoleranceandfunctionanalysisofzmgst42geneinmaize AT liuxinyue integratedtranscriptomicandproteomicanalysesoflownitrogenstresstoleranceandfunctionanalysisofzmgst42geneinmaize AT wangnan integratedtranscriptomicandproteomicanalysesoflownitrogenstresstoleranceandfunctionanalysisofzmgst42geneinmaize AT duanhuijun integratedtranscriptomicandproteomicanalysesoflownitrogenstresstoleranceandfunctionanalysisofzmgst42geneinmaize |