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Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying

SIMPLE SUMMARY: Feather replacement is one of the most typical features of fasting-induced physiological remodeling, but the specific mechanism is unknown. By observing the changes in feathers and hair follicles throughout the process, this study reveals that molting can increase the market value of...

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Autores principales: Zhang, Lujie, Cai, Chunxia, Liu, Xinxin, Zhang, Xiaoran, An, Zhiyuan, Zhou, Enyou, Li, Jianzeng, Li, Zhuanjian, Li, Wenting, Sun, Guirong, Li, Guoxi, Kang, Xiangtao, Han, Ruili, Jiang, Ruirui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10603888/
https://www.ncbi.nlm.nih.gov/pubmed/37887055
http://dx.doi.org/10.3390/biology12101345
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author Zhang, Lujie
Cai, Chunxia
Liu, Xinxin
Zhang, Xiaoran
An, Zhiyuan
Zhou, Enyou
Li, Jianzeng
Li, Zhuanjian
Li, Wenting
Sun, Guirong
Li, Guoxi
Kang, Xiangtao
Han, Ruili
Jiang, Ruirui
author_facet Zhang, Lujie
Cai, Chunxia
Liu, Xinxin
Zhang, Xiaoran
An, Zhiyuan
Zhou, Enyou
Li, Jianzeng
Li, Zhuanjian
Li, Wenting
Sun, Guirong
Li, Guoxi
Kang, Xiangtao
Han, Ruili
Jiang, Ruirui
author_sort Zhang, Lujie
collection PubMed
description SIMPLE SUMMARY: Feather replacement is one of the most typical features of fasting-induced physiological remodeling, but the specific mechanism is unknown. By observing the changes in feathers and hair follicles throughout the process, this study reveals that molting can increase the market value of culled laying hens and improve the carcass appearance of chilled chickens. In addition, combined with transcriptome sequencing, candidate genes related to hair follicle development were found, namely DSP, CDH1, PKP1, etc., and a specific pathway elucidating how thyroid hormone affects feathering was proposed. These data provide a valuable resource for the analysis of the molecular mechanisms underlying the cyclical growth of hair follicles in the skin during induced molting. ABSTRACT: Induced molting is a common method to obtain a new life in laying hens, in which periodic changes in feathers are the prominent feature. Nevertheless, its precise molecular mechanism remains unclear. In this study, feather and hair follicle samples were collected during fasting-induced physiological remodeling for hematoxylin–eosin staining, hormone changes and follicle traits, and transcriptome sequencing. Feather shedding was observed in F13 to R25, while newborns were observed in R3 to R32. Triiodothyronine and tetraiodothyronine were significantly elevated during feather shedding. The calcium content was significantly higher, and the ash content was significantly lower after the changeover. The determination of hair follicle traits revealed an increasing trend in pore density and a decrease in pore diameter after the resumption of feeding. According to RNA-seq results, several core genes were identified, including DSP, CDH1, PKP1, and PPCKB, which may have an impact on hair follicle growth. The focus was to discover that starvation may trigger changes in thyroid hormones, which in turn regulate feather molting through thyroid hormone synthesis, calcium signaling, and thyroid hormone signaling pathways. These data provide a valuable resource for the analysis of the molecular mechanisms underlying the cyclical growth of hair follicles in the skin during induced molting.
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spelling pubmed-106038882023-10-28 Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying Zhang, Lujie Cai, Chunxia Liu, Xinxin Zhang, Xiaoran An, Zhiyuan Zhou, Enyou Li, Jianzeng Li, Zhuanjian Li, Wenting Sun, Guirong Li, Guoxi Kang, Xiangtao Han, Ruili Jiang, Ruirui Biology (Basel) Article SIMPLE SUMMARY: Feather replacement is one of the most typical features of fasting-induced physiological remodeling, but the specific mechanism is unknown. By observing the changes in feathers and hair follicles throughout the process, this study reveals that molting can increase the market value of culled laying hens and improve the carcass appearance of chilled chickens. In addition, combined with transcriptome sequencing, candidate genes related to hair follicle development were found, namely DSP, CDH1, PKP1, etc., and a specific pathway elucidating how thyroid hormone affects feathering was proposed. These data provide a valuable resource for the analysis of the molecular mechanisms underlying the cyclical growth of hair follicles in the skin during induced molting. ABSTRACT: Induced molting is a common method to obtain a new life in laying hens, in which periodic changes in feathers are the prominent feature. Nevertheless, its precise molecular mechanism remains unclear. In this study, feather and hair follicle samples were collected during fasting-induced physiological remodeling for hematoxylin–eosin staining, hormone changes and follicle traits, and transcriptome sequencing. Feather shedding was observed in F13 to R25, while newborns were observed in R3 to R32. Triiodothyronine and tetraiodothyronine were significantly elevated during feather shedding. The calcium content was significantly higher, and the ash content was significantly lower after the changeover. The determination of hair follicle traits revealed an increasing trend in pore density and a decrease in pore diameter after the resumption of feeding. According to RNA-seq results, several core genes were identified, including DSP, CDH1, PKP1, and PPCKB, which may have an impact on hair follicle growth. The focus was to discover that starvation may trigger changes in thyroid hormones, which in turn regulate feather molting through thyroid hormone synthesis, calcium signaling, and thyroid hormone signaling pathways. These data provide a valuable resource for the analysis of the molecular mechanisms underlying the cyclical growth of hair follicles in the skin during induced molting. MDPI 2023-10-19 /pmc/articles/PMC10603888/ /pubmed/37887055 http://dx.doi.org/10.3390/biology12101345 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Lujie
Cai, Chunxia
Liu, Xinxin
Zhang, Xiaoran
An, Zhiyuan
Zhou, Enyou
Li, Jianzeng
Li, Zhuanjian
Li, Wenting
Sun, Guirong
Li, Guoxi
Kang, Xiangtao
Han, Ruili
Jiang, Ruirui
Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying
title Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying
title_full Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying
title_fullStr Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying
title_full_unstemmed Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying
title_short Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying
title_sort multi-stage transcriptome analysis revealed the growth mechanism of feathers and hair follicles during induction molting by fasting in the late stage of egg laying
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10603888/
https://www.ncbi.nlm.nih.gov/pubmed/37887055
http://dx.doi.org/10.3390/biology12101345
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