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Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD)

Periapical odontogenic granulomas are among the most encountered pathology that involve the alveolar bone, with severe consequences such as bone resorption, the presence of inflammatory infiltrate and the formation of abnormal vascularization. The present study aimed to quantify the existence of the...

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Autores principales: Roi, Ciprian, Negruțiu, Meda Lavinia, Roi, Alexandra, Riviș, Mircea, Luca, Ruxandra Elena, Raica, Marius, Ceaușu, Raluca Amalia, Motofelea, Alexandru Cătălin, Gaje, Pușa Nela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10603906/
https://www.ncbi.nlm.nih.gov/pubmed/37893083
http://dx.doi.org/10.3390/biomedicines11102709
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author Roi, Ciprian
Negruțiu, Meda Lavinia
Roi, Alexandra
Riviș, Mircea
Luca, Ruxandra Elena
Raica, Marius
Ceaușu, Raluca Amalia
Motofelea, Alexandru Cătălin
Gaje, Pușa Nela
author_facet Roi, Ciprian
Negruțiu, Meda Lavinia
Roi, Alexandra
Riviș, Mircea
Luca, Ruxandra Elena
Raica, Marius
Ceaușu, Raluca Amalia
Motofelea, Alexandru Cătălin
Gaje, Pușa Nela
author_sort Roi, Ciprian
collection PubMed
description Periapical odontogenic granulomas are among the most encountered pathology that involve the alveolar bone, with severe consequences such as bone resorption, the presence of inflammatory infiltrate and the formation of abnormal vascularization. The present study aimed to quantify the existence of the microvessel density (MVD), mast cell density (MCD) and heterogeneity of the encountered blood vessels. A total of 37 patients diagnosed with odontogenic periapical granulomas were included, and the gender distribution, age and localization of the pathological lesions was assessed. After the surgical removal of the periapical odontogenic granuloma, the collected tissue was fixed in 10% buffered formalin. Primary processing, morphological analysis and immunohistochemical staining was performed in order to characterize the altered tissue. The results outlined the presence of a high number of mast cells, especially in the area of the inflamed tissue; the high heterogeneity of the blood vessels; and increased MVD with positive CD34. The conclusions of the study focus on the key role of the mast cells and their implication in the initiation and development of the angiogenesis process, triggering the inflammatory response of the host. Nevertheless, periapical odontogenic granulomas develop as an inflammatory response to the interaction between the host’s immune system and microbial invasion.
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spelling pubmed-106039062023-10-28 Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD) Roi, Ciprian Negruțiu, Meda Lavinia Roi, Alexandra Riviș, Mircea Luca, Ruxandra Elena Raica, Marius Ceaușu, Raluca Amalia Motofelea, Alexandru Cătălin Gaje, Pușa Nela Biomedicines Article Periapical odontogenic granulomas are among the most encountered pathology that involve the alveolar bone, with severe consequences such as bone resorption, the presence of inflammatory infiltrate and the formation of abnormal vascularization. The present study aimed to quantify the existence of the microvessel density (MVD), mast cell density (MCD) and heterogeneity of the encountered blood vessels. A total of 37 patients diagnosed with odontogenic periapical granulomas were included, and the gender distribution, age and localization of the pathological lesions was assessed. After the surgical removal of the periapical odontogenic granuloma, the collected tissue was fixed in 10% buffered formalin. Primary processing, morphological analysis and immunohistochemical staining was performed in order to characterize the altered tissue. The results outlined the presence of a high number of mast cells, especially in the area of the inflamed tissue; the high heterogeneity of the blood vessels; and increased MVD with positive CD34. The conclusions of the study focus on the key role of the mast cells and their implication in the initiation and development of the angiogenesis process, triggering the inflammatory response of the host. Nevertheless, periapical odontogenic granulomas develop as an inflammatory response to the interaction between the host’s immune system and microbial invasion. MDPI 2023-10-05 /pmc/articles/PMC10603906/ /pubmed/37893083 http://dx.doi.org/10.3390/biomedicines11102709 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Roi, Ciprian
Negruțiu, Meda Lavinia
Roi, Alexandra
Riviș, Mircea
Luca, Ruxandra Elena
Raica, Marius
Ceaușu, Raluca Amalia
Motofelea, Alexandru Cătălin
Gaje, Pușa Nela
Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD)
title Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD)
title_full Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD)
title_fullStr Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD)
title_full_unstemmed Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD)
title_short Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD)
title_sort human periapical odontogenic granulomas: aspects of microvessel density (mvd), heterogeneity of blood vessels and mast cells density (mcd)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10603906/
https://www.ncbi.nlm.nih.gov/pubmed/37893083
http://dx.doi.org/10.3390/biomedicines11102709
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