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Amphiphilic Particle-Stabilized Nanoliter Droplet Reactors with a Multimodal Portable Reader for Distributive Biomarker Quantification

[Image: see text] Compartmentalization, leveraging microfluidics, enables highly sensitive assays, but the requirement for significant infrastructure for their design, build, and operation limits access. Multimaterial particle-based technologies thermodynamically stabilize monodisperse droplets as i...

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Autores principales: Shah, Vishwesh, Yang, Xilin, Arnheim, Alyssa, Udani, Shreya, Tseng, Derek, Luo, Yi, Ouyang, Mengxing, Destgeer, Ghulam, Garner, Omai B., Koydemir, Hatice C., Ozcan, Aydogan, Di Carlo, Dino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10604076/
https://www.ncbi.nlm.nih.gov/pubmed/37824510
http://dx.doi.org/10.1021/acsnano.3c04994
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author Shah, Vishwesh
Yang, Xilin
Arnheim, Alyssa
Udani, Shreya
Tseng, Derek
Luo, Yi
Ouyang, Mengxing
Destgeer, Ghulam
Garner, Omai B.
Koydemir, Hatice C.
Ozcan, Aydogan
Di Carlo, Dino
author_facet Shah, Vishwesh
Yang, Xilin
Arnheim, Alyssa
Udani, Shreya
Tseng, Derek
Luo, Yi
Ouyang, Mengxing
Destgeer, Ghulam
Garner, Omai B.
Koydemir, Hatice C.
Ozcan, Aydogan
Di Carlo, Dino
author_sort Shah, Vishwesh
collection PubMed
description [Image: see text] Compartmentalization, leveraging microfluidics, enables highly sensitive assays, but the requirement for significant infrastructure for their design, build, and operation limits access. Multimaterial particle-based technologies thermodynamically stabilize monodisperse droplets as individual reaction compartments with simple liquid handling steps, precluding the need for expensive microfluidic equipment. Here, we further improve the accessibility of this lab on a particle technology to resource-limited settings by combining this assay system with a portable multimodal reader, thus enabling nanoliter droplet assays in an accessible platform. We show the utility of this platform in measuring N-terminal propeptide B-type natriuretic peptide (NT-proBNP), a heart failure biomarker, in complex medium and patient samples. We report a limit of detection of ∼0.05 ng/mL and a linear response between 0.2 and 2 ng/mL in spiked plasma samples. We also show that, owing to the plurality of measurements per sample, “swarm” sensing acquires better statistical quantitation with a portable reader. Monte Carlo simulations show the increasing capability of this platform to differentiate between negative and positive samples, i.e., below or above the clinical cutoff for acute heart failure (∼0.1 ng/mL), as a function of the number of particles measured. Our platform measurements correlate with gold standard ELISA measurement in cardiac patient samples, and achieve lower variation in measurement across samples compared to the standard well plate-based ELISA. Thus, we show the capabilities of a cost-effective droplet-reader system in accurately measuring biomarkers in nanoliter droplets for diseases that disproportionately affect underserved communities in resource-limited settings.
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spelling pubmed-106040762023-10-28 Amphiphilic Particle-Stabilized Nanoliter Droplet Reactors with a Multimodal Portable Reader for Distributive Biomarker Quantification Shah, Vishwesh Yang, Xilin Arnheim, Alyssa Udani, Shreya Tseng, Derek Luo, Yi Ouyang, Mengxing Destgeer, Ghulam Garner, Omai B. Koydemir, Hatice C. Ozcan, Aydogan Di Carlo, Dino ACS Nano [Image: see text] Compartmentalization, leveraging microfluidics, enables highly sensitive assays, but the requirement for significant infrastructure for their design, build, and operation limits access. Multimaterial particle-based technologies thermodynamically stabilize monodisperse droplets as individual reaction compartments with simple liquid handling steps, precluding the need for expensive microfluidic equipment. Here, we further improve the accessibility of this lab on a particle technology to resource-limited settings by combining this assay system with a portable multimodal reader, thus enabling nanoliter droplet assays in an accessible platform. We show the utility of this platform in measuring N-terminal propeptide B-type natriuretic peptide (NT-proBNP), a heart failure biomarker, in complex medium and patient samples. We report a limit of detection of ∼0.05 ng/mL and a linear response between 0.2 and 2 ng/mL in spiked plasma samples. We also show that, owing to the plurality of measurements per sample, “swarm” sensing acquires better statistical quantitation with a portable reader. Monte Carlo simulations show the increasing capability of this platform to differentiate between negative and positive samples, i.e., below or above the clinical cutoff for acute heart failure (∼0.1 ng/mL), as a function of the number of particles measured. Our platform measurements correlate with gold standard ELISA measurement in cardiac patient samples, and achieve lower variation in measurement across samples compared to the standard well plate-based ELISA. Thus, we show the capabilities of a cost-effective droplet-reader system in accurately measuring biomarkers in nanoliter droplets for diseases that disproportionately affect underserved communities in resource-limited settings. American Chemical Society 2023-10-12 /pmc/articles/PMC10604076/ /pubmed/37824510 http://dx.doi.org/10.1021/acsnano.3c04994 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Shah, Vishwesh
Yang, Xilin
Arnheim, Alyssa
Udani, Shreya
Tseng, Derek
Luo, Yi
Ouyang, Mengxing
Destgeer, Ghulam
Garner, Omai B.
Koydemir, Hatice C.
Ozcan, Aydogan
Di Carlo, Dino
Amphiphilic Particle-Stabilized Nanoliter Droplet Reactors with a Multimodal Portable Reader for Distributive Biomarker Quantification
title Amphiphilic Particle-Stabilized Nanoliter Droplet Reactors with a Multimodal Portable Reader for Distributive Biomarker Quantification
title_full Amphiphilic Particle-Stabilized Nanoliter Droplet Reactors with a Multimodal Portable Reader for Distributive Biomarker Quantification
title_fullStr Amphiphilic Particle-Stabilized Nanoliter Droplet Reactors with a Multimodal Portable Reader for Distributive Biomarker Quantification
title_full_unstemmed Amphiphilic Particle-Stabilized Nanoliter Droplet Reactors with a Multimodal Portable Reader for Distributive Biomarker Quantification
title_short Amphiphilic Particle-Stabilized Nanoliter Droplet Reactors with a Multimodal Portable Reader for Distributive Biomarker Quantification
title_sort amphiphilic particle-stabilized nanoliter droplet reactors with a multimodal portable reader for distributive biomarker quantification
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10604076/
https://www.ncbi.nlm.nih.gov/pubmed/37824510
http://dx.doi.org/10.1021/acsnano.3c04994
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