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Quantitative Insights and Visualization of Antimicrobial Tolerance in Mixed-Species Biofilms
Biofilms are a major problem in hard-to-heal wounds. Moreover, they are composed of different species and are often tolerant to antimicrobial agents. At the same time, interspecific synergy and/or competition occurs when some bacterial species clash. For this reason, the tolerance of two dual-specie...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10604264/ https://www.ncbi.nlm.nih.gov/pubmed/37893014 http://dx.doi.org/10.3390/biomedicines11102640 |
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author | Dittmer, Mandy Brill, Florian H. H. Kampe, Andreas Geffken, Maria Rembe, Julian-Dario Moll, Raphael Alio, Ifey Streit, Wolfgang R. Debus, Eike Sebastian Smeets, Ralf Stuermer, Ewa Klara |
author_facet | Dittmer, Mandy Brill, Florian H. H. Kampe, Andreas Geffken, Maria Rembe, Julian-Dario Moll, Raphael Alio, Ifey Streit, Wolfgang R. Debus, Eike Sebastian Smeets, Ralf Stuermer, Ewa Klara |
author_sort | Dittmer, Mandy |
collection | PubMed |
description | Biofilms are a major problem in hard-to-heal wounds. Moreover, they are composed of different species and are often tolerant to antimicrobial agents. At the same time, interspecific synergy and/or competition occurs when some bacterial species clash. For this reason, the tolerance of two dual-species wound biofilm models of Pseudomonas aeruginosa and Staphylococcus aureus or Enterococcus faecium against antimicrobials and antimicrobial dressings were analyzed quantitatively and by confocal laser scanning microscopy (CLSM). The results were compared to findings with planktonic bacteria. Octenidine-dihydrochloride/phenoxyethanol and polyhexamethylene biguanide (PHMB) irrigation solutions showed a significant, albeit delayed reduction in biofilm bacteria, while the PHMB dressing was not able to induce this effect. However, the cadexomer-iodine dressing caused a sustained reduction in and killed almost all bacteria down to 10(2) cfu/mL within 6 days compared to the control (10(10) cfu/mL). By means of CLSM in untreated human biofilm models, it became evident that P. aeruginosa dominates over E. faecium and S. aureus. Additionally, P. aeruginosa appeared as a vast layer at the bottom of the samples, while S. aureus formed grape-like clusters. In the second model, the distribution was even clearer. Only a few E. faecium were visible, in contrast to the vast layer of P. aeruginosa. It seems that the different species avoid each other and seek their respective niches. These mixed-species biofilm models showed that efficacy and tolerance to antimicrobial substances are nearly species-independent. Their frequent application appears to be important. The bacterial wound biofilm remains a challenge in treatment and requires new, combined therapy options. |
format | Online Article Text |
id | pubmed-10604264 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106042642023-10-28 Quantitative Insights and Visualization of Antimicrobial Tolerance in Mixed-Species Biofilms Dittmer, Mandy Brill, Florian H. H. Kampe, Andreas Geffken, Maria Rembe, Julian-Dario Moll, Raphael Alio, Ifey Streit, Wolfgang R. Debus, Eike Sebastian Smeets, Ralf Stuermer, Ewa Klara Biomedicines Article Biofilms are a major problem in hard-to-heal wounds. Moreover, they are composed of different species and are often tolerant to antimicrobial agents. At the same time, interspecific synergy and/or competition occurs when some bacterial species clash. For this reason, the tolerance of two dual-species wound biofilm models of Pseudomonas aeruginosa and Staphylococcus aureus or Enterococcus faecium against antimicrobials and antimicrobial dressings were analyzed quantitatively and by confocal laser scanning microscopy (CLSM). The results were compared to findings with planktonic bacteria. Octenidine-dihydrochloride/phenoxyethanol and polyhexamethylene biguanide (PHMB) irrigation solutions showed a significant, albeit delayed reduction in biofilm bacteria, while the PHMB dressing was not able to induce this effect. However, the cadexomer-iodine dressing caused a sustained reduction in and killed almost all bacteria down to 10(2) cfu/mL within 6 days compared to the control (10(10) cfu/mL). By means of CLSM in untreated human biofilm models, it became evident that P. aeruginosa dominates over E. faecium and S. aureus. Additionally, P. aeruginosa appeared as a vast layer at the bottom of the samples, while S. aureus formed grape-like clusters. In the second model, the distribution was even clearer. Only a few E. faecium were visible, in contrast to the vast layer of P. aeruginosa. It seems that the different species avoid each other and seek their respective niches. These mixed-species biofilm models showed that efficacy and tolerance to antimicrobial substances are nearly species-independent. Their frequent application appears to be important. The bacterial wound biofilm remains a challenge in treatment and requires new, combined therapy options. MDPI 2023-09-26 /pmc/articles/PMC10604264/ /pubmed/37893014 http://dx.doi.org/10.3390/biomedicines11102640 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dittmer, Mandy Brill, Florian H. H. Kampe, Andreas Geffken, Maria Rembe, Julian-Dario Moll, Raphael Alio, Ifey Streit, Wolfgang R. Debus, Eike Sebastian Smeets, Ralf Stuermer, Ewa Klara Quantitative Insights and Visualization of Antimicrobial Tolerance in Mixed-Species Biofilms |
title | Quantitative Insights and Visualization of Antimicrobial Tolerance in Mixed-Species Biofilms |
title_full | Quantitative Insights and Visualization of Antimicrobial Tolerance in Mixed-Species Biofilms |
title_fullStr | Quantitative Insights and Visualization of Antimicrobial Tolerance in Mixed-Species Biofilms |
title_full_unstemmed | Quantitative Insights and Visualization of Antimicrobial Tolerance in Mixed-Species Biofilms |
title_short | Quantitative Insights and Visualization of Antimicrobial Tolerance in Mixed-Species Biofilms |
title_sort | quantitative insights and visualization of antimicrobial tolerance in mixed-species biofilms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10604264/ https://www.ncbi.nlm.nih.gov/pubmed/37893014 http://dx.doi.org/10.3390/biomedicines11102640 |
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