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Genomic Landscape of Multidrug Resistance and Virulence in Enterococcus faecalis IRMC827A from a Long-Term Patient

SIMPLE SUMMARY: A highly virulent, multidrug-resistant Enterococcus faecalis IRMC827A strain was found in a Saudi Arabian hospital. The strain carries antimicrobial resistance genes and mobile genetic elements, making it resistant to various antibiotics. It also carries virulence factors associated...

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Autores principales: Borgio, J. Francis, AlJindan, Reem, Alghourab, Lujeen H., Alquwaie, Rahaf, Aldahhan, Razan, Alhur, Norah F., AlEraky, Doaa M., Mahmoud, Nehal, Almandil, Noor B., AbdulAzeez, Sayed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10604365/
https://www.ncbi.nlm.nih.gov/pubmed/37887006
http://dx.doi.org/10.3390/biology12101296
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author Borgio, J. Francis
AlJindan, Reem
Alghourab, Lujeen H.
Alquwaie, Rahaf
Aldahhan, Razan
Alhur, Norah F.
AlEraky, Doaa M.
Mahmoud, Nehal
Almandil, Noor B.
AbdulAzeez, Sayed
author_facet Borgio, J. Francis
AlJindan, Reem
Alghourab, Lujeen H.
Alquwaie, Rahaf
Aldahhan, Razan
Alhur, Norah F.
AlEraky, Doaa M.
Mahmoud, Nehal
Almandil, Noor B.
AbdulAzeez, Sayed
author_sort Borgio, J. Francis
collection PubMed
description SIMPLE SUMMARY: A highly virulent, multidrug-resistant Enterococcus faecalis IRMC827A strain was found in a Saudi Arabian hospital. The strain carries antimicrobial resistance genes and mobile genetic elements, making it resistant to various antibiotics. It also carries virulence factors associated with adherence, biofilm formation, and spreading multidrug resistance. The study highlights the importance of monitoring multidrug-resistant E. faecalis colonization and infection in hospitalized patients, as it is a serious pathogen. ABSTRACT: We report on a highly virulent, multidrug-resistant strain of Enterococcus faecalis IRMC827A that was found colonizing a long-term male patient at a tertiary hospital in Khobar, Saudi Arabia. The E. faecalis IRMC827A strain carries several antimicrobial drug resistance genes and harbours mobile genetic elements such as Tn6009, which is an integrative conjugative element that can transfer resistance genes between bacteria and ISS1N via an insertion sequence. Whole-genome-sequencing-based antimicrobial susceptibility testing on strains from faecal samples revealed that the isolate E. faecalis IRMC827A is highly resistant to a variety of antibiotics, including tetracycline, doxycycline, minocycline, dalfopristin, virginiamycin, pristinamycin, chloramphenicol, streptomycin, clindamycin, lincomycin, trimethoprim, nalidixic acid and ciprofloxacin. The isolate IRMC827A carries several virulence factors that are significantly associated with adherence, biofilm formation, sortase-assembled pili, manganese uptake, antiphagocytosis, and spreading factor of multidrug resistance. The isolate also encompasses two mutations (G2576T and G2505A) in the 23S rRNA gene associated with linezolid resistance and three more mutations (gyrA p.S83Y, gyrA p.D759N and parC p.S80I) of the antimicrobial resistance phenotype. The findings through next-generation sequencing on the resistome, mobilome and virulome of the isolate in the study highlight the significance of monitoring multidrug-resistant E. faecalis colonization and infection in hospitalized patients. As multidrug-resistant E. faecalis is a serious pathogen, it is particularly difficult to treat and can cause fatal infections. It is important to have quick and accurate diagnostic tests for multidrug-resistant E. faecalis, to track the spread of multidrug-resistant E. faecalis in healthcare settings, and to improve targeted interventions to stop its spread. Further research is necessary to develop novel antibiotics and treatment strategies for multidrug-resistant E. faecalis infections.
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spelling pubmed-106043652023-10-28 Genomic Landscape of Multidrug Resistance and Virulence in Enterococcus faecalis IRMC827A from a Long-Term Patient Borgio, J. Francis AlJindan, Reem Alghourab, Lujeen H. Alquwaie, Rahaf Aldahhan, Razan Alhur, Norah F. AlEraky, Doaa M. Mahmoud, Nehal Almandil, Noor B. AbdulAzeez, Sayed Biology (Basel) Article SIMPLE SUMMARY: A highly virulent, multidrug-resistant Enterococcus faecalis IRMC827A strain was found in a Saudi Arabian hospital. The strain carries antimicrobial resistance genes and mobile genetic elements, making it resistant to various antibiotics. It also carries virulence factors associated with adherence, biofilm formation, and spreading multidrug resistance. The study highlights the importance of monitoring multidrug-resistant E. faecalis colonization and infection in hospitalized patients, as it is a serious pathogen. ABSTRACT: We report on a highly virulent, multidrug-resistant strain of Enterococcus faecalis IRMC827A that was found colonizing a long-term male patient at a tertiary hospital in Khobar, Saudi Arabia. The E. faecalis IRMC827A strain carries several antimicrobial drug resistance genes and harbours mobile genetic elements such as Tn6009, which is an integrative conjugative element that can transfer resistance genes between bacteria and ISS1N via an insertion sequence. Whole-genome-sequencing-based antimicrobial susceptibility testing on strains from faecal samples revealed that the isolate E. faecalis IRMC827A is highly resistant to a variety of antibiotics, including tetracycline, doxycycline, minocycline, dalfopristin, virginiamycin, pristinamycin, chloramphenicol, streptomycin, clindamycin, lincomycin, trimethoprim, nalidixic acid and ciprofloxacin. The isolate IRMC827A carries several virulence factors that are significantly associated with adherence, biofilm formation, sortase-assembled pili, manganese uptake, antiphagocytosis, and spreading factor of multidrug resistance. The isolate also encompasses two mutations (G2576T and G2505A) in the 23S rRNA gene associated with linezolid resistance and three more mutations (gyrA p.S83Y, gyrA p.D759N and parC p.S80I) of the antimicrobial resistance phenotype. The findings through next-generation sequencing on the resistome, mobilome and virulome of the isolate in the study highlight the significance of monitoring multidrug-resistant E. faecalis colonization and infection in hospitalized patients. As multidrug-resistant E. faecalis is a serious pathogen, it is particularly difficult to treat and can cause fatal infections. It is important to have quick and accurate diagnostic tests for multidrug-resistant E. faecalis, to track the spread of multidrug-resistant E. faecalis in healthcare settings, and to improve targeted interventions to stop its spread. Further research is necessary to develop novel antibiotics and treatment strategies for multidrug-resistant E. faecalis infections. MDPI 2023-09-29 /pmc/articles/PMC10604365/ /pubmed/37887006 http://dx.doi.org/10.3390/biology12101296 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Borgio, J. Francis
AlJindan, Reem
Alghourab, Lujeen H.
Alquwaie, Rahaf
Aldahhan, Razan
Alhur, Norah F.
AlEraky, Doaa M.
Mahmoud, Nehal
Almandil, Noor B.
AbdulAzeez, Sayed
Genomic Landscape of Multidrug Resistance and Virulence in Enterococcus faecalis IRMC827A from a Long-Term Patient
title Genomic Landscape of Multidrug Resistance and Virulence in Enterococcus faecalis IRMC827A from a Long-Term Patient
title_full Genomic Landscape of Multidrug Resistance and Virulence in Enterococcus faecalis IRMC827A from a Long-Term Patient
title_fullStr Genomic Landscape of Multidrug Resistance and Virulence in Enterococcus faecalis IRMC827A from a Long-Term Patient
title_full_unstemmed Genomic Landscape of Multidrug Resistance and Virulence in Enterococcus faecalis IRMC827A from a Long-Term Patient
title_short Genomic Landscape of Multidrug Resistance and Virulence in Enterococcus faecalis IRMC827A from a Long-Term Patient
title_sort genomic landscape of multidrug resistance and virulence in enterococcus faecalis irmc827a from a long-term patient
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10604365/
https://www.ncbi.nlm.nih.gov/pubmed/37887006
http://dx.doi.org/10.3390/biology12101296
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