Cargando…
Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome
Currently, multiple studies have indicated that CD8+ T lymphocytes play a role in causing damage to the exocrine glands through acinar injury in primary Sjögren’s syndrome (pSS). The aim of this research was to assess the imbalance of circulating CD8+ T cell subsets. We analyzed blood samples from 3...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10604770/ https://www.ncbi.nlm.nih.gov/pubmed/37893153 http://dx.doi.org/10.3390/biomedicines11102778 |
_version_ | 1785126914706374656 |
---|---|
author | Kudryavtsev, Igor Benevolenskaya, Stanislava Serebriakova, Maria Grigor’yeva, Irina Kuvardin, Evgeniy Rubinstein, Artem Golovkin, Alexey Kalinina, Olga Zaikova, Ekaterina Lapin, Sergey Maslyanskiy, Alexey |
author_facet | Kudryavtsev, Igor Benevolenskaya, Stanislava Serebriakova, Maria Grigor’yeva, Irina Kuvardin, Evgeniy Rubinstein, Artem Golovkin, Alexey Kalinina, Olga Zaikova, Ekaterina Lapin, Sergey Maslyanskiy, Alexey |
author_sort | Kudryavtsev, Igor |
collection | PubMed |
description | Currently, multiple studies have indicated that CD8+ T lymphocytes play a role in causing damage to the exocrine glands through acinar injury in primary Sjögren’s syndrome (pSS). The aim of this research was to assess the imbalance of circulating CD8+ T cell subsets. We analyzed blood samples from 34 pSS patients and 34 healthy individuals as controls. We used flow cytometry to enumerate CD8+ T cell maturation stages, using as markers CD62L, CD28, CD27, CD4, CD8, CD3, CD45RA and CD45. For immunophenotyping of ‘polarized’ CD8+ T cell subsets, we used the following monoclonal antibodies: CXCR5, CCR6, CXCR3 and CCR4. The findings revealed that both the relative and absolute numbers of ‘naïve’ CD8+ T cells were higher in pSS patients compared to the healthy volunteers. Conversely, the proportions of effector memory CD8+ T cells were notably lower. Furthermore, our data suggested that among patients with pSS, the levels of cytotoxic Tc1 CD8+ T cells were reduced, while the frequencies of regulatory cytokine-producing Tc2 and Tc17 CD8+ T cells were significantly elevated. Simultaneously, the Tc1 cell subsets displayed a negative correlation with immunoglobulin G, rheumatoid factor, the Schirmer test and unstimulated saliva flow. On the other hand, the Tc2 cell subsets exhibited a positive correlation with these parameters. In summary, our study indicated that immune dysfunction within CD8+ T cells, including alterations in Tc1 cells, plays a significant role in the development of pSS. |
format | Online Article Text |
id | pubmed-10604770 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106047702023-10-28 Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome Kudryavtsev, Igor Benevolenskaya, Stanislava Serebriakova, Maria Grigor’yeva, Irina Kuvardin, Evgeniy Rubinstein, Artem Golovkin, Alexey Kalinina, Olga Zaikova, Ekaterina Lapin, Sergey Maslyanskiy, Alexey Biomedicines Article Currently, multiple studies have indicated that CD8+ T lymphocytes play a role in causing damage to the exocrine glands through acinar injury in primary Sjögren’s syndrome (pSS). The aim of this research was to assess the imbalance of circulating CD8+ T cell subsets. We analyzed blood samples from 34 pSS patients and 34 healthy individuals as controls. We used flow cytometry to enumerate CD8+ T cell maturation stages, using as markers CD62L, CD28, CD27, CD4, CD8, CD3, CD45RA and CD45. For immunophenotyping of ‘polarized’ CD8+ T cell subsets, we used the following monoclonal antibodies: CXCR5, CCR6, CXCR3 and CCR4. The findings revealed that both the relative and absolute numbers of ‘naïve’ CD8+ T cells were higher in pSS patients compared to the healthy volunteers. Conversely, the proportions of effector memory CD8+ T cells were notably lower. Furthermore, our data suggested that among patients with pSS, the levels of cytotoxic Tc1 CD8+ T cells were reduced, while the frequencies of regulatory cytokine-producing Tc2 and Tc17 CD8+ T cells were significantly elevated. Simultaneously, the Tc1 cell subsets displayed a negative correlation with immunoglobulin G, rheumatoid factor, the Schirmer test and unstimulated saliva flow. On the other hand, the Tc2 cell subsets exhibited a positive correlation with these parameters. In summary, our study indicated that immune dysfunction within CD8+ T cells, including alterations in Tc1 cells, plays a significant role in the development of pSS. MDPI 2023-10-13 /pmc/articles/PMC10604770/ /pubmed/37893153 http://dx.doi.org/10.3390/biomedicines11102778 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kudryavtsev, Igor Benevolenskaya, Stanislava Serebriakova, Maria Grigor’yeva, Irina Kuvardin, Evgeniy Rubinstein, Artem Golovkin, Alexey Kalinina, Olga Zaikova, Ekaterina Lapin, Sergey Maslyanskiy, Alexey Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome |
title | Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome |
title_full | Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome |
title_fullStr | Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome |
title_full_unstemmed | Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome |
title_short | Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome |
title_sort | circulating cd8+ t cell subsets in primary sjögren’s syndrome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10604770/ https://www.ncbi.nlm.nih.gov/pubmed/37893153 http://dx.doi.org/10.3390/biomedicines11102778 |
work_keys_str_mv | AT kudryavtsevigor circulatingcd8tcellsubsetsinprimarysjogrenssyndrome AT benevolenskayastanislava circulatingcd8tcellsubsetsinprimarysjogrenssyndrome AT serebriakovamaria circulatingcd8tcellsubsetsinprimarysjogrenssyndrome AT grigoryevairina circulatingcd8tcellsubsetsinprimarysjogrenssyndrome AT kuvardinevgeniy circulatingcd8tcellsubsetsinprimarysjogrenssyndrome AT rubinsteinartem circulatingcd8tcellsubsetsinprimarysjogrenssyndrome AT golovkinalexey circulatingcd8tcellsubsetsinprimarysjogrenssyndrome AT kalininaolga circulatingcd8tcellsubsetsinprimarysjogrenssyndrome AT zaikovaekaterina circulatingcd8tcellsubsetsinprimarysjogrenssyndrome AT lapinsergey circulatingcd8tcellsubsetsinprimarysjogrenssyndrome AT maslyanskiyalexey circulatingcd8tcellsubsetsinprimarysjogrenssyndrome |