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Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome

Currently, multiple studies have indicated that CD8+ T lymphocytes play a role in causing damage to the exocrine glands through acinar injury in primary Sjögren’s syndrome (pSS). The aim of this research was to assess the imbalance of circulating CD8+ T cell subsets. We analyzed blood samples from 3...

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Autores principales: Kudryavtsev, Igor, Benevolenskaya, Stanislava, Serebriakova, Maria, Grigor’yeva, Irina, Kuvardin, Evgeniy, Rubinstein, Artem, Golovkin, Alexey, Kalinina, Olga, Zaikova, Ekaterina, Lapin, Sergey, Maslyanskiy, Alexey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10604770/
https://www.ncbi.nlm.nih.gov/pubmed/37893153
http://dx.doi.org/10.3390/biomedicines11102778
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author Kudryavtsev, Igor
Benevolenskaya, Stanislava
Serebriakova, Maria
Grigor’yeva, Irina
Kuvardin, Evgeniy
Rubinstein, Artem
Golovkin, Alexey
Kalinina, Olga
Zaikova, Ekaterina
Lapin, Sergey
Maslyanskiy, Alexey
author_facet Kudryavtsev, Igor
Benevolenskaya, Stanislava
Serebriakova, Maria
Grigor’yeva, Irina
Kuvardin, Evgeniy
Rubinstein, Artem
Golovkin, Alexey
Kalinina, Olga
Zaikova, Ekaterina
Lapin, Sergey
Maslyanskiy, Alexey
author_sort Kudryavtsev, Igor
collection PubMed
description Currently, multiple studies have indicated that CD8+ T lymphocytes play a role in causing damage to the exocrine glands through acinar injury in primary Sjögren’s syndrome (pSS). The aim of this research was to assess the imbalance of circulating CD8+ T cell subsets. We analyzed blood samples from 34 pSS patients and 34 healthy individuals as controls. We used flow cytometry to enumerate CD8+ T cell maturation stages, using as markers CD62L, CD28, CD27, CD4, CD8, CD3, CD45RA and CD45. For immunophenotyping of ‘polarized’ CD8+ T cell subsets, we used the following monoclonal antibodies: CXCR5, CCR6, CXCR3 and CCR4. The findings revealed that both the relative and absolute numbers of ‘naïve’ CD8+ T cells were higher in pSS patients compared to the healthy volunteers. Conversely, the proportions of effector memory CD8+ T cells were notably lower. Furthermore, our data suggested that among patients with pSS, the levels of cytotoxic Tc1 CD8+ T cells were reduced, while the frequencies of regulatory cytokine-producing Tc2 and Tc17 CD8+ T cells were significantly elevated. Simultaneously, the Tc1 cell subsets displayed a negative correlation with immunoglobulin G, rheumatoid factor, the Schirmer test and unstimulated saliva flow. On the other hand, the Tc2 cell subsets exhibited a positive correlation with these parameters. In summary, our study indicated that immune dysfunction within CD8+ T cells, including alterations in Tc1 cells, plays a significant role in the development of pSS.
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spelling pubmed-106047702023-10-28 Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome Kudryavtsev, Igor Benevolenskaya, Stanislava Serebriakova, Maria Grigor’yeva, Irina Kuvardin, Evgeniy Rubinstein, Artem Golovkin, Alexey Kalinina, Olga Zaikova, Ekaterina Lapin, Sergey Maslyanskiy, Alexey Biomedicines Article Currently, multiple studies have indicated that CD8+ T lymphocytes play a role in causing damage to the exocrine glands through acinar injury in primary Sjögren’s syndrome (pSS). The aim of this research was to assess the imbalance of circulating CD8+ T cell subsets. We analyzed blood samples from 34 pSS patients and 34 healthy individuals as controls. We used flow cytometry to enumerate CD8+ T cell maturation stages, using as markers CD62L, CD28, CD27, CD4, CD8, CD3, CD45RA and CD45. For immunophenotyping of ‘polarized’ CD8+ T cell subsets, we used the following monoclonal antibodies: CXCR5, CCR6, CXCR3 and CCR4. The findings revealed that both the relative and absolute numbers of ‘naïve’ CD8+ T cells were higher in pSS patients compared to the healthy volunteers. Conversely, the proportions of effector memory CD8+ T cells were notably lower. Furthermore, our data suggested that among patients with pSS, the levels of cytotoxic Tc1 CD8+ T cells were reduced, while the frequencies of regulatory cytokine-producing Tc2 and Tc17 CD8+ T cells were significantly elevated. Simultaneously, the Tc1 cell subsets displayed a negative correlation with immunoglobulin G, rheumatoid factor, the Schirmer test and unstimulated saliva flow. On the other hand, the Tc2 cell subsets exhibited a positive correlation with these parameters. In summary, our study indicated that immune dysfunction within CD8+ T cells, including alterations in Tc1 cells, plays a significant role in the development of pSS. MDPI 2023-10-13 /pmc/articles/PMC10604770/ /pubmed/37893153 http://dx.doi.org/10.3390/biomedicines11102778 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kudryavtsev, Igor
Benevolenskaya, Stanislava
Serebriakova, Maria
Grigor’yeva, Irina
Kuvardin, Evgeniy
Rubinstein, Artem
Golovkin, Alexey
Kalinina, Olga
Zaikova, Ekaterina
Lapin, Sergey
Maslyanskiy, Alexey
Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome
title Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome
title_full Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome
title_fullStr Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome
title_full_unstemmed Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome
title_short Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome
title_sort circulating cd8+ t cell subsets in primary sjögren’s syndrome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10604770/
https://www.ncbi.nlm.nih.gov/pubmed/37893153
http://dx.doi.org/10.3390/biomedicines11102778
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