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Laminin α4 Expression in Human Adipose Tissue Depots and Its Association with Obesity and Obesity Related Traits

Laminin α4 (LAMA4) is one of the main structural adipocyte basement membrane (BM) components that is upregulated during adipogenesis and related to obesity in mice and humans. We conducted RNA-seq-based gene expression analysis of LAMA4 in abdominal subcutaneous (SC) and visceral (VIS) adipose tissu...

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Autores principales: Hagemann, Tobias, Czechowski, Paul, Ghosh, Adhideb, Sun, Wenfei, Dong, Hua, Noé, Falko, Wolfrum, Christian, Blüher, Matthias, Hoffmann, Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10604865/
https://www.ncbi.nlm.nih.gov/pubmed/37893179
http://dx.doi.org/10.3390/biomedicines11102806
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author Hagemann, Tobias
Czechowski, Paul
Ghosh, Adhideb
Sun, Wenfei
Dong, Hua
Noé, Falko
Wolfrum, Christian
Blüher, Matthias
Hoffmann, Anne
author_facet Hagemann, Tobias
Czechowski, Paul
Ghosh, Adhideb
Sun, Wenfei
Dong, Hua
Noé, Falko
Wolfrum, Christian
Blüher, Matthias
Hoffmann, Anne
author_sort Hagemann, Tobias
collection PubMed
description Laminin α4 (LAMA4) is one of the main structural adipocyte basement membrane (BM) components that is upregulated during adipogenesis and related to obesity in mice and humans. We conducted RNA-seq-based gene expression analysis of LAMA4 in abdominal subcutaneous (SC) and visceral (VIS) adipose tissue (AT) depots across three human sub-cohorts of the Leipzig Obesity BioBank (LOBB) to explore the relationship between LAMA4 expression and obesity (N = 1479) in the context of weight loss (N = 65) and metabolic health (N = 42). We found significant associations of LAMA4 with body fat mass (p < 0.001) in VIS AT; higher expression in VIS AT compared to SC AT; and significant relation to metabolic health parameters e.g., body fat in VIS AT, waist (p = 0.009) and interleukin 6 (p = 0.002) in male VIS AT, and hemoglobin A1c (p = 0.008) in male SC AT. AT LAMA4 expression was not significantly different between subjects with or without obesity, metabolically healthy versus unhealthy, and obesity before versus after short-term weight loss. Our results support significant associations between obesity related clinical parameters and elevated LAMA4 expression in humans. Our work offers one of the first references for understanding the meaning of LAMA4 expression specifically in relation to obesity based on large-scale RNA-seq data.
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spelling pubmed-106048652023-10-28 Laminin α4 Expression in Human Adipose Tissue Depots and Its Association with Obesity and Obesity Related Traits Hagemann, Tobias Czechowski, Paul Ghosh, Adhideb Sun, Wenfei Dong, Hua Noé, Falko Wolfrum, Christian Blüher, Matthias Hoffmann, Anne Biomedicines Brief Report Laminin α4 (LAMA4) is one of the main structural adipocyte basement membrane (BM) components that is upregulated during adipogenesis and related to obesity in mice and humans. We conducted RNA-seq-based gene expression analysis of LAMA4 in abdominal subcutaneous (SC) and visceral (VIS) adipose tissue (AT) depots across three human sub-cohorts of the Leipzig Obesity BioBank (LOBB) to explore the relationship between LAMA4 expression and obesity (N = 1479) in the context of weight loss (N = 65) and metabolic health (N = 42). We found significant associations of LAMA4 with body fat mass (p < 0.001) in VIS AT; higher expression in VIS AT compared to SC AT; and significant relation to metabolic health parameters e.g., body fat in VIS AT, waist (p = 0.009) and interleukin 6 (p = 0.002) in male VIS AT, and hemoglobin A1c (p = 0.008) in male SC AT. AT LAMA4 expression was not significantly different between subjects with or without obesity, metabolically healthy versus unhealthy, and obesity before versus after short-term weight loss. Our results support significant associations between obesity related clinical parameters and elevated LAMA4 expression in humans. Our work offers one of the first references for understanding the meaning of LAMA4 expression specifically in relation to obesity based on large-scale RNA-seq data. MDPI 2023-10-17 /pmc/articles/PMC10604865/ /pubmed/37893179 http://dx.doi.org/10.3390/biomedicines11102806 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Hagemann, Tobias
Czechowski, Paul
Ghosh, Adhideb
Sun, Wenfei
Dong, Hua
Noé, Falko
Wolfrum, Christian
Blüher, Matthias
Hoffmann, Anne
Laminin α4 Expression in Human Adipose Tissue Depots and Its Association with Obesity and Obesity Related Traits
title Laminin α4 Expression in Human Adipose Tissue Depots and Its Association with Obesity and Obesity Related Traits
title_full Laminin α4 Expression in Human Adipose Tissue Depots and Its Association with Obesity and Obesity Related Traits
title_fullStr Laminin α4 Expression in Human Adipose Tissue Depots and Its Association with Obesity and Obesity Related Traits
title_full_unstemmed Laminin α4 Expression in Human Adipose Tissue Depots and Its Association with Obesity and Obesity Related Traits
title_short Laminin α4 Expression in Human Adipose Tissue Depots and Its Association with Obesity and Obesity Related Traits
title_sort laminin α4 expression in human adipose tissue depots and its association with obesity and obesity related traits
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10604865/
https://www.ncbi.nlm.nih.gov/pubmed/37893179
http://dx.doi.org/10.3390/biomedicines11102806
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