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Hyperthermia Enhances Adeno-Associated Virus Vector Transduction Efficiency in Melanoma Cells
Gene therapy perfectly fits in the current needs of medicine for patients with melanoma. One of the major challenges of gene therapy is to increase gene transfer. The role of hyperthermia in the improvement of AAV (adeno-associated virus) transduction efficiency has been indicated. The aim of the pr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10604982/ https://www.ncbi.nlm.nih.gov/pubmed/37886980 http://dx.doi.org/10.3390/cimb45100537 |
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author | Bieńkowska-Tokarczyk, Alicja Stelmaszczyk-Emmel, Anna Demkow, Urszula Małecki, Maciej |
author_facet | Bieńkowska-Tokarczyk, Alicja Stelmaszczyk-Emmel, Anna Demkow, Urszula Małecki, Maciej |
author_sort | Bieńkowska-Tokarczyk, Alicja |
collection | PubMed |
description | Gene therapy perfectly fits in the current needs of medicine for patients with melanoma. One of the major challenges of gene therapy is to increase gene transfer. The role of hyperthermia in the improvement of AAV (adeno-associated virus) transduction efficiency has been indicated. The aim of the present study was to assess the transduction efficacy of melanoma cell lines (A375, G-361, and SK-MEL-1) with the use of the rAAV/DJ mosaic vector under hyperthermia conditions. The analysis of changes in the transduction efficacy and expression of HSPs (heat shock proteins) and receptors for AAV was performed. The transduction was performed at 37 °C and at 43 °C (1 h). Hyperthermia enhanced gene transfer in all the tested cell lines. The most efficient transducing cell line under hyperthermia was A375 (increase by 17%). G361 and SK-MEL-1 cells showed an increase of 7%. The changes in the expression of the AAV receptors and HSPs after hyperthermia were observed. A key role in the improvement of gene transfer may be played by AAVR, HSPB1, HSP6, DNAJC4, HSPD1, HSPA8, HSPA9, HSP90AB1, and AHSA1. This study showed the possibility of the use of hyperthermia as a factor enabling the stimulation of cell transduction with rAAV vectors, thereby providing tools for the improvement in the efficacy of gene therapy based on rAAV. |
format | Online Article Text |
id | pubmed-10604982 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106049822023-10-28 Hyperthermia Enhances Adeno-Associated Virus Vector Transduction Efficiency in Melanoma Cells Bieńkowska-Tokarczyk, Alicja Stelmaszczyk-Emmel, Anna Demkow, Urszula Małecki, Maciej Curr Issues Mol Biol Article Gene therapy perfectly fits in the current needs of medicine for patients with melanoma. One of the major challenges of gene therapy is to increase gene transfer. The role of hyperthermia in the improvement of AAV (adeno-associated virus) transduction efficiency has been indicated. The aim of the present study was to assess the transduction efficacy of melanoma cell lines (A375, G-361, and SK-MEL-1) with the use of the rAAV/DJ mosaic vector under hyperthermia conditions. The analysis of changes in the transduction efficacy and expression of HSPs (heat shock proteins) and receptors for AAV was performed. The transduction was performed at 37 °C and at 43 °C (1 h). Hyperthermia enhanced gene transfer in all the tested cell lines. The most efficient transducing cell line under hyperthermia was A375 (increase by 17%). G361 and SK-MEL-1 cells showed an increase of 7%. The changes in the expression of the AAV receptors and HSPs after hyperthermia were observed. A key role in the improvement of gene transfer may be played by AAVR, HSPB1, HSP6, DNAJC4, HSPD1, HSPA8, HSPA9, HSP90AB1, and AHSA1. This study showed the possibility of the use of hyperthermia as a factor enabling the stimulation of cell transduction with rAAV vectors, thereby providing tools for the improvement in the efficacy of gene therapy based on rAAV. MDPI 2023-10-23 /pmc/articles/PMC10604982/ /pubmed/37886980 http://dx.doi.org/10.3390/cimb45100537 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bieńkowska-Tokarczyk, Alicja Stelmaszczyk-Emmel, Anna Demkow, Urszula Małecki, Maciej Hyperthermia Enhances Adeno-Associated Virus Vector Transduction Efficiency in Melanoma Cells |
title | Hyperthermia Enhances Adeno-Associated Virus Vector Transduction Efficiency in Melanoma Cells |
title_full | Hyperthermia Enhances Adeno-Associated Virus Vector Transduction Efficiency in Melanoma Cells |
title_fullStr | Hyperthermia Enhances Adeno-Associated Virus Vector Transduction Efficiency in Melanoma Cells |
title_full_unstemmed | Hyperthermia Enhances Adeno-Associated Virus Vector Transduction Efficiency in Melanoma Cells |
title_short | Hyperthermia Enhances Adeno-Associated Virus Vector Transduction Efficiency in Melanoma Cells |
title_sort | hyperthermia enhances adeno-associated virus vector transduction efficiency in melanoma cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10604982/ https://www.ncbi.nlm.nih.gov/pubmed/37886980 http://dx.doi.org/10.3390/cimb45100537 |
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