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Surface-Enhanced Raman Scattering Active Core-Shell Ag NPs@Carbon Dots with Enzyme-Mimicking Activities for Label-Free Measurement Cholesterol

Serological-sensitive testing of cholesterol holds significant value in the fields of healthcare and clinical diagnosis. This study reports on the preparation of peroxidase-mimicking nanozymes through the wrapping of N, S-doped carbon dots (DCDs) on the surface of silver nanoparticles (Ag NPs@DCD)....

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Detalles Bibliográficos
Autores principales: Ju, Jian, Li, Lin, Li, Bei, Regmi, Sagar, Wang, Tingting, Xu, Jiao, Li, Chaojie, Tang, Shixing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10605028/
https://www.ncbi.nlm.nih.gov/pubmed/37887120
http://dx.doi.org/10.3390/bios13100927
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author Ju, Jian
Li, Lin
Li, Bei
Regmi, Sagar
Wang, Tingting
Xu, Jiao
Li, Chaojie
Tang, Shixing
author_facet Ju, Jian
Li, Lin
Li, Bei
Regmi, Sagar
Wang, Tingting
Xu, Jiao
Li, Chaojie
Tang, Shixing
author_sort Ju, Jian
collection PubMed
description Serological-sensitive testing of cholesterol holds significant value in the fields of healthcare and clinical diagnosis. This study reports on the preparation of peroxidase-mimicking nanozymes through the wrapping of N, S-doped carbon dots (DCDs) on the surface of silver nanoparticles (Ag NPs@DCD). The shell–core structure of Ag NPs@DCD displays peroxidase-mimicking capability, with the potential to catalyze inactive Raman probe molecules into the Raman reporters. Furthermore, a “shell-isolated nanoparticles-enhanced Raman spectroscopy” structure exhibited an enhanced Raman signal of reporter molecules. Ag NPs@DCD were utilized to create a label-free SERS sensing system for high-performance detection of cholesterol in serum samples. These results demonstrate the potential of the novel nanozyme-based SERS approach for clinical diagnosis.
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spelling pubmed-106050282023-10-28 Surface-Enhanced Raman Scattering Active Core-Shell Ag NPs@Carbon Dots with Enzyme-Mimicking Activities for Label-Free Measurement Cholesterol Ju, Jian Li, Lin Li, Bei Regmi, Sagar Wang, Tingting Xu, Jiao Li, Chaojie Tang, Shixing Biosensors (Basel) Communication Serological-sensitive testing of cholesterol holds significant value in the fields of healthcare and clinical diagnosis. This study reports on the preparation of peroxidase-mimicking nanozymes through the wrapping of N, S-doped carbon dots (DCDs) on the surface of silver nanoparticles (Ag NPs@DCD). The shell–core structure of Ag NPs@DCD displays peroxidase-mimicking capability, with the potential to catalyze inactive Raman probe molecules into the Raman reporters. Furthermore, a “shell-isolated nanoparticles-enhanced Raman spectroscopy” structure exhibited an enhanced Raman signal of reporter molecules. Ag NPs@DCD were utilized to create a label-free SERS sensing system for high-performance detection of cholesterol in serum samples. These results demonstrate the potential of the novel nanozyme-based SERS approach for clinical diagnosis. MDPI 2023-10-16 /pmc/articles/PMC10605028/ /pubmed/37887120 http://dx.doi.org/10.3390/bios13100927 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Ju, Jian
Li, Lin
Li, Bei
Regmi, Sagar
Wang, Tingting
Xu, Jiao
Li, Chaojie
Tang, Shixing
Surface-Enhanced Raman Scattering Active Core-Shell Ag NPs@Carbon Dots with Enzyme-Mimicking Activities for Label-Free Measurement Cholesterol
title Surface-Enhanced Raman Scattering Active Core-Shell Ag NPs@Carbon Dots with Enzyme-Mimicking Activities for Label-Free Measurement Cholesterol
title_full Surface-Enhanced Raman Scattering Active Core-Shell Ag NPs@Carbon Dots with Enzyme-Mimicking Activities for Label-Free Measurement Cholesterol
title_fullStr Surface-Enhanced Raman Scattering Active Core-Shell Ag NPs@Carbon Dots with Enzyme-Mimicking Activities for Label-Free Measurement Cholesterol
title_full_unstemmed Surface-Enhanced Raman Scattering Active Core-Shell Ag NPs@Carbon Dots with Enzyme-Mimicking Activities for Label-Free Measurement Cholesterol
title_short Surface-Enhanced Raman Scattering Active Core-Shell Ag NPs@Carbon Dots with Enzyme-Mimicking Activities for Label-Free Measurement Cholesterol
title_sort surface-enhanced raman scattering active core-shell ag nps@carbon dots with enzyme-mimicking activities for label-free measurement cholesterol
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10605028/
https://www.ncbi.nlm.nih.gov/pubmed/37887120
http://dx.doi.org/10.3390/bios13100927
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