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Surface-Enhanced Raman Scattering Active Core-Shell Ag NPs@Carbon Dots with Enzyme-Mimicking Activities for Label-Free Measurement Cholesterol
Serological-sensitive testing of cholesterol holds significant value in the fields of healthcare and clinical diagnosis. This study reports on the preparation of peroxidase-mimicking nanozymes through the wrapping of N, S-doped carbon dots (DCDs) on the surface of silver nanoparticles (Ag NPs@DCD)....
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10605028/ https://www.ncbi.nlm.nih.gov/pubmed/37887120 http://dx.doi.org/10.3390/bios13100927 |
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author | Ju, Jian Li, Lin Li, Bei Regmi, Sagar Wang, Tingting Xu, Jiao Li, Chaojie Tang, Shixing |
author_facet | Ju, Jian Li, Lin Li, Bei Regmi, Sagar Wang, Tingting Xu, Jiao Li, Chaojie Tang, Shixing |
author_sort | Ju, Jian |
collection | PubMed |
description | Serological-sensitive testing of cholesterol holds significant value in the fields of healthcare and clinical diagnosis. This study reports on the preparation of peroxidase-mimicking nanozymes through the wrapping of N, S-doped carbon dots (DCDs) on the surface of silver nanoparticles (Ag NPs@DCD). The shell–core structure of Ag NPs@DCD displays peroxidase-mimicking capability, with the potential to catalyze inactive Raman probe molecules into the Raman reporters. Furthermore, a “shell-isolated nanoparticles-enhanced Raman spectroscopy” structure exhibited an enhanced Raman signal of reporter molecules. Ag NPs@DCD were utilized to create a label-free SERS sensing system for high-performance detection of cholesterol in serum samples. These results demonstrate the potential of the novel nanozyme-based SERS approach for clinical diagnosis. |
format | Online Article Text |
id | pubmed-10605028 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106050282023-10-28 Surface-Enhanced Raman Scattering Active Core-Shell Ag NPs@Carbon Dots with Enzyme-Mimicking Activities for Label-Free Measurement Cholesterol Ju, Jian Li, Lin Li, Bei Regmi, Sagar Wang, Tingting Xu, Jiao Li, Chaojie Tang, Shixing Biosensors (Basel) Communication Serological-sensitive testing of cholesterol holds significant value in the fields of healthcare and clinical diagnosis. This study reports on the preparation of peroxidase-mimicking nanozymes through the wrapping of N, S-doped carbon dots (DCDs) on the surface of silver nanoparticles (Ag NPs@DCD). The shell–core structure of Ag NPs@DCD displays peroxidase-mimicking capability, with the potential to catalyze inactive Raman probe molecules into the Raman reporters. Furthermore, a “shell-isolated nanoparticles-enhanced Raman spectroscopy” structure exhibited an enhanced Raman signal of reporter molecules. Ag NPs@DCD were utilized to create a label-free SERS sensing system for high-performance detection of cholesterol in serum samples. These results demonstrate the potential of the novel nanozyme-based SERS approach for clinical diagnosis. MDPI 2023-10-16 /pmc/articles/PMC10605028/ /pubmed/37887120 http://dx.doi.org/10.3390/bios13100927 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Ju, Jian Li, Lin Li, Bei Regmi, Sagar Wang, Tingting Xu, Jiao Li, Chaojie Tang, Shixing Surface-Enhanced Raman Scattering Active Core-Shell Ag NPs@Carbon Dots with Enzyme-Mimicking Activities for Label-Free Measurement Cholesterol |
title | Surface-Enhanced Raman Scattering Active Core-Shell Ag NPs@Carbon Dots with Enzyme-Mimicking Activities for Label-Free Measurement Cholesterol |
title_full | Surface-Enhanced Raman Scattering Active Core-Shell Ag NPs@Carbon Dots with Enzyme-Mimicking Activities for Label-Free Measurement Cholesterol |
title_fullStr | Surface-Enhanced Raman Scattering Active Core-Shell Ag NPs@Carbon Dots with Enzyme-Mimicking Activities for Label-Free Measurement Cholesterol |
title_full_unstemmed | Surface-Enhanced Raman Scattering Active Core-Shell Ag NPs@Carbon Dots with Enzyme-Mimicking Activities for Label-Free Measurement Cholesterol |
title_short | Surface-Enhanced Raman Scattering Active Core-Shell Ag NPs@Carbon Dots with Enzyme-Mimicking Activities for Label-Free Measurement Cholesterol |
title_sort | surface-enhanced raman scattering active core-shell ag nps@carbon dots with enzyme-mimicking activities for label-free measurement cholesterol |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10605028/ https://www.ncbi.nlm.nih.gov/pubmed/37887120 http://dx.doi.org/10.3390/bios13100927 |
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