Development of an Anti-HER2 Single-Chain Variable Antibody Fragment Construct for High-Yield Soluble Expression in Escherichia coli and One-Step Chromatographic Purification

Although single-chain variable fragment (scFv) is recognized as a highly versatile scaffold of recombinant antibody fragment molecules, its overexpression in Escherichia coli often leads to the formation of inclusion bodies. To address this issue, we devised and tested four different constructs, nam...

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Detalles Bibliográficos
Autores principales: Byun, Kyu Tae, Kim, Boram, Cho, Junmin, Lee, Inbeom, Lee, Myung Gu, Park, Dongsun, Kang, Tae-Bong, Won, Hyung-Sik, Kim, Chan Gil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10605039/
https://www.ncbi.nlm.nih.gov/pubmed/37892190
http://dx.doi.org/10.3390/biom13101508

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