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Electrical Characterization and Analysis of Single Cells and Related Applications
Biological parameters extracted from electrical signals from various body parts have been used for many years to analyze the human body and its behavior. In addition, electrical signals from cancer cell lines, normal cells, and viruses, among others, have been widely used for the detection of variou...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10605054/ https://www.ncbi.nlm.nih.gov/pubmed/37887100 http://dx.doi.org/10.3390/bios13100907 |
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author | Zhu, Weitao Wang, Jiaao Luo, Hongzhi Luo, Binwen Li, Xue Liu, Shan Li, Chenzhong |
author_facet | Zhu, Weitao Wang, Jiaao Luo, Hongzhi Luo, Binwen Li, Xue Liu, Shan Li, Chenzhong |
author_sort | Zhu, Weitao |
collection | PubMed |
description | Biological parameters extracted from electrical signals from various body parts have been used for many years to analyze the human body and its behavior. In addition, electrical signals from cancer cell lines, normal cells, and viruses, among others, have been widely used for the detection of various diseases. Single-cell parameters such as cell and cytoplasmic conductivity, relaxation frequency, and membrane capacitance are important. There are many techniques available to characterize biomaterials, such as nanotechnology, microstrip cavity resonance measurement, etc. This article reviews single-cell isolation and sorting techniques, such as the micropipette separation method, separation and sorting system (dual electrophoretic array system), DEPArray sorting system (dielectrophoretic array system), cell selector sorting system, and microfluidic and valve devices, and discusses their respective advantages and disadvantages. Furthermore, it summarizes common single-cell electrical manipulations, such as single-cell amperometry (SCA), electrical impedance sensing (EIS), impedance flow cytometry (IFC), cell-based electrical impedance (CEI), microelectromechanical systems (MEMS), and integrated microelectrode array (IMA). The article also enumerates the application and significance of single-cell electrochemical analysis from the perspectives of CTC liquid biopsy, recombinant adenovirus, tumor cells like lung cancer DTCs (LC-DTCs), and single-cell metabolomics analysis. The paper concludes with a discussion of the current limitations faced by single-cell analysis techniques along with future directions and potential application scenarios. |
format | Online Article Text |
id | pubmed-10605054 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106050542023-10-28 Electrical Characterization and Analysis of Single Cells and Related Applications Zhu, Weitao Wang, Jiaao Luo, Hongzhi Luo, Binwen Li, Xue Liu, Shan Li, Chenzhong Biosensors (Basel) Review Biological parameters extracted from electrical signals from various body parts have been used for many years to analyze the human body and its behavior. In addition, electrical signals from cancer cell lines, normal cells, and viruses, among others, have been widely used for the detection of various diseases. Single-cell parameters such as cell and cytoplasmic conductivity, relaxation frequency, and membrane capacitance are important. There are many techniques available to characterize biomaterials, such as nanotechnology, microstrip cavity resonance measurement, etc. This article reviews single-cell isolation and sorting techniques, such as the micropipette separation method, separation and sorting system (dual electrophoretic array system), DEPArray sorting system (dielectrophoretic array system), cell selector sorting system, and microfluidic and valve devices, and discusses their respective advantages and disadvantages. Furthermore, it summarizes common single-cell electrical manipulations, such as single-cell amperometry (SCA), electrical impedance sensing (EIS), impedance flow cytometry (IFC), cell-based electrical impedance (CEI), microelectromechanical systems (MEMS), and integrated microelectrode array (IMA). The article also enumerates the application and significance of single-cell electrochemical analysis from the perspectives of CTC liquid biopsy, recombinant adenovirus, tumor cells like lung cancer DTCs (LC-DTCs), and single-cell metabolomics analysis. The paper concludes with a discussion of the current limitations faced by single-cell analysis techniques along with future directions and potential application scenarios. MDPI 2023-09-26 /pmc/articles/PMC10605054/ /pubmed/37887100 http://dx.doi.org/10.3390/bios13100907 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Zhu, Weitao Wang, Jiaao Luo, Hongzhi Luo, Binwen Li, Xue Liu, Shan Li, Chenzhong Electrical Characterization and Analysis of Single Cells and Related Applications |
title | Electrical Characterization and Analysis of Single Cells and Related Applications |
title_full | Electrical Characterization and Analysis of Single Cells and Related Applications |
title_fullStr | Electrical Characterization and Analysis of Single Cells and Related Applications |
title_full_unstemmed | Electrical Characterization and Analysis of Single Cells and Related Applications |
title_short | Electrical Characterization and Analysis of Single Cells and Related Applications |
title_sort | electrical characterization and analysis of single cells and related applications |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10605054/ https://www.ncbi.nlm.nih.gov/pubmed/37887100 http://dx.doi.org/10.3390/bios13100907 |
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