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Super-Resolution Ultrasound Imaging of Renal Vascular Alterations in Zucker Diabetic Fatty Rats during the Development of Diabetic Kidney Disease

Individuals with diabetes at risk of developing diabetic kidney disease (DKD) are challenging to identify using currently available clinical methods. Prognostic accuracy and initiation of treatment could be improved by a quantification of the renal microvascular rarefaction and the increased vascula...

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Autores principales: Søgaard, Stinne Byrholdt, Andersen, Sofie Bech, Taghavi, Iman, Schou, Mikkel, Christoffersen, Christina, Jacobsen, Jens Christian Brings, Kjer, Hans Martin, Gundlach, Carsten, McDermott, Amy, Jensen, Jørgen Arendt, Nielsen, Michael Bachmann, Sørensen, Charlotte Mehlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10605617/
https://www.ncbi.nlm.nih.gov/pubmed/37892017
http://dx.doi.org/10.3390/diagnostics13203197
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author Søgaard, Stinne Byrholdt
Andersen, Sofie Bech
Taghavi, Iman
Schou, Mikkel
Christoffersen, Christina
Jacobsen, Jens Christian Brings
Kjer, Hans Martin
Gundlach, Carsten
McDermott, Amy
Jensen, Jørgen Arendt
Nielsen, Michael Bachmann
Sørensen, Charlotte Mehlin
author_facet Søgaard, Stinne Byrholdt
Andersen, Sofie Bech
Taghavi, Iman
Schou, Mikkel
Christoffersen, Christina
Jacobsen, Jens Christian Brings
Kjer, Hans Martin
Gundlach, Carsten
McDermott, Amy
Jensen, Jørgen Arendt
Nielsen, Michael Bachmann
Sørensen, Charlotte Mehlin
author_sort Søgaard, Stinne Byrholdt
collection PubMed
description Individuals with diabetes at risk of developing diabetic kidney disease (DKD) are challenging to identify using currently available clinical methods. Prognostic accuracy and initiation of treatment could be improved by a quantification of the renal microvascular rarefaction and the increased vascular tortuosity during the development of DKD. Super-resolution ultrasound (SRUS) imaging is an in vivo technique capable of visualizing blood vessels at sizes below 75 µm. This preclinical study aimed to investigate the alterations in renal blood vessels’ density and tortuosity in a type 2 diabetes rat model, Zucker diabetic fatty (ZDF) rats, as a prediction of DKD. Lean age-matched Zucker rats were used as controls. A total of 36 rats were studied, subdivided into ages of 12, 22, and 40 weeks. Measured albuminuria indicated the early stage of DKD, and the SRUS was compared with the ex vivo micro-computed tomography (µCT) of the same kidneys. Assessed using the SRUS imaging, a significantly decreased cortical vascular density was detected in the ZDF rats from 22 weeks of age compared to the healthy controls, concomitant with a significantly increased albuminuria. Already by week 12, a trend towards a decreased cortical vascular density was found prior to the increased albuminuria. The quantified vascular density in µCT corresponded with the in vivo SRUS imaging, presenting a consistently lower vascular density in the ZDF rats. Regarding vessel tortuosity, an overall trend towards an increased tortuosity was present in the ZDF rats. SRUS shows promise for becoming an additional tool for monitoring and prognosing DKD. In the future, large-scale animal studies and human trials are needed for confirmation.
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spelling pubmed-106056172023-10-28 Super-Resolution Ultrasound Imaging of Renal Vascular Alterations in Zucker Diabetic Fatty Rats during the Development of Diabetic Kidney Disease Søgaard, Stinne Byrholdt Andersen, Sofie Bech Taghavi, Iman Schou, Mikkel Christoffersen, Christina Jacobsen, Jens Christian Brings Kjer, Hans Martin Gundlach, Carsten McDermott, Amy Jensen, Jørgen Arendt Nielsen, Michael Bachmann Sørensen, Charlotte Mehlin Diagnostics (Basel) Article Individuals with diabetes at risk of developing diabetic kidney disease (DKD) are challenging to identify using currently available clinical methods. Prognostic accuracy and initiation of treatment could be improved by a quantification of the renal microvascular rarefaction and the increased vascular tortuosity during the development of DKD. Super-resolution ultrasound (SRUS) imaging is an in vivo technique capable of visualizing blood vessels at sizes below 75 µm. This preclinical study aimed to investigate the alterations in renal blood vessels’ density and tortuosity in a type 2 diabetes rat model, Zucker diabetic fatty (ZDF) rats, as a prediction of DKD. Lean age-matched Zucker rats were used as controls. A total of 36 rats were studied, subdivided into ages of 12, 22, and 40 weeks. Measured albuminuria indicated the early stage of DKD, and the SRUS was compared with the ex vivo micro-computed tomography (µCT) of the same kidneys. Assessed using the SRUS imaging, a significantly decreased cortical vascular density was detected in the ZDF rats from 22 weeks of age compared to the healthy controls, concomitant with a significantly increased albuminuria. Already by week 12, a trend towards a decreased cortical vascular density was found prior to the increased albuminuria. The quantified vascular density in µCT corresponded with the in vivo SRUS imaging, presenting a consistently lower vascular density in the ZDF rats. Regarding vessel tortuosity, an overall trend towards an increased tortuosity was present in the ZDF rats. SRUS shows promise for becoming an additional tool for monitoring and prognosing DKD. In the future, large-scale animal studies and human trials are needed for confirmation. MDPI 2023-10-12 /pmc/articles/PMC10605617/ /pubmed/37892017 http://dx.doi.org/10.3390/diagnostics13203197 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Søgaard, Stinne Byrholdt
Andersen, Sofie Bech
Taghavi, Iman
Schou, Mikkel
Christoffersen, Christina
Jacobsen, Jens Christian Brings
Kjer, Hans Martin
Gundlach, Carsten
McDermott, Amy
Jensen, Jørgen Arendt
Nielsen, Michael Bachmann
Sørensen, Charlotte Mehlin
Super-Resolution Ultrasound Imaging of Renal Vascular Alterations in Zucker Diabetic Fatty Rats during the Development of Diabetic Kidney Disease
title Super-Resolution Ultrasound Imaging of Renal Vascular Alterations in Zucker Diabetic Fatty Rats during the Development of Diabetic Kidney Disease
title_full Super-Resolution Ultrasound Imaging of Renal Vascular Alterations in Zucker Diabetic Fatty Rats during the Development of Diabetic Kidney Disease
title_fullStr Super-Resolution Ultrasound Imaging of Renal Vascular Alterations in Zucker Diabetic Fatty Rats during the Development of Diabetic Kidney Disease
title_full_unstemmed Super-Resolution Ultrasound Imaging of Renal Vascular Alterations in Zucker Diabetic Fatty Rats during the Development of Diabetic Kidney Disease
title_short Super-Resolution Ultrasound Imaging of Renal Vascular Alterations in Zucker Diabetic Fatty Rats during the Development of Diabetic Kidney Disease
title_sort super-resolution ultrasound imaging of renal vascular alterations in zucker diabetic fatty rats during the development of diabetic kidney disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10605617/
https://www.ncbi.nlm.nih.gov/pubmed/37892017
http://dx.doi.org/10.3390/diagnostics13203197
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