Metabolites of non-aureus staphylococci affect the ability of Staphylococcus aureus to adhere to and internalize into bovine mammary epithelial cells

This study investigated whether cell-free supernatants (SN) from four bovine non-aureus staphylococcal (NAS) isolates prevent Staphylococcus aureus adhesion to and internalization into bovine mammary epithelial cells (MAC-T cells) and if so, to determine whether such effects were potentially associa...

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Autores principales: Toledo-Silva, Bruno, Oliveira, Ana Cláudia Dumont, Souza, Fernando N., Haesebrouck, Freddy, De Vliegher, Sarne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10605872/
https://www.ncbi.nlm.nih.gov/pubmed/37884947
http://dx.doi.org/10.1186/s13567-023-01232-3
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author Toledo-Silva, Bruno
Oliveira, Ana Cláudia Dumont
Souza, Fernando N.
Haesebrouck, Freddy
De Vliegher, Sarne
author_facet Toledo-Silva, Bruno
Oliveira, Ana Cláudia Dumont
Souza, Fernando N.
Haesebrouck, Freddy
De Vliegher, Sarne
author_sort Toledo-Silva, Bruno
collection PubMed
description This study investigated whether cell-free supernatants (SN) from four bovine non-aureus staphylococcal (NAS) isolates prevent Staphylococcus aureus adhesion to and internalization into bovine mammary epithelial cells (MAC-T cells) and if so, to determine whether such effects were potentially associated with the S. aureus accessory gene regulator (agr) system. Overall, we demonstrated that all SN obtained from the NAS isolates promoted adhesion of a S. aureus agr(+) strain to, yet reduced the internalization into MAC-T cells, while similar effects were not observed for its agr(−) mutant strain. Our findings provide novel anti-virulence strategies for treating and controlling bovine S. aureus mastitis.
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spelling pubmed-106058722023-10-28 Metabolites of non-aureus staphylococci affect the ability of Staphylococcus aureus to adhere to and internalize into bovine mammary epithelial cells Toledo-Silva, Bruno Oliveira, Ana Cláudia Dumont Souza, Fernando N. Haesebrouck, Freddy De Vliegher, Sarne Vet Res Short Report This study investigated whether cell-free supernatants (SN) from four bovine non-aureus staphylococcal (NAS) isolates prevent Staphylococcus aureus adhesion to and internalization into bovine mammary epithelial cells (MAC-T cells) and if so, to determine whether such effects were potentially associated with the S. aureus accessory gene regulator (agr) system. Overall, we demonstrated that all SN obtained from the NAS isolates promoted adhesion of a S. aureus agr(+) strain to, yet reduced the internalization into MAC-T cells, while similar effects were not observed for its agr(−) mutant strain. Our findings provide novel anti-virulence strategies for treating and controlling bovine S. aureus mastitis. BioMed Central 2023-10-26 2023 /pmc/articles/PMC10605872/ /pubmed/37884947 http://dx.doi.org/10.1186/s13567-023-01232-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Toledo-Silva, Bruno
Oliveira, Ana Cláudia Dumont
Souza, Fernando N.
Haesebrouck, Freddy
De Vliegher, Sarne
Metabolites of non-aureus staphylococci affect the ability of Staphylococcus aureus to adhere to and internalize into bovine mammary epithelial cells
title Metabolites of non-aureus staphylococci affect the ability of Staphylococcus aureus to adhere to and internalize into bovine mammary epithelial cells
title_full Metabolites of non-aureus staphylococci affect the ability of Staphylococcus aureus to adhere to and internalize into bovine mammary epithelial cells
title_fullStr Metabolites of non-aureus staphylococci affect the ability of Staphylococcus aureus to adhere to and internalize into bovine mammary epithelial cells
title_full_unstemmed Metabolites of non-aureus staphylococci affect the ability of Staphylococcus aureus to adhere to and internalize into bovine mammary epithelial cells
title_short Metabolites of non-aureus staphylococci affect the ability of Staphylococcus aureus to adhere to and internalize into bovine mammary epithelial cells
title_sort metabolites of non-aureus staphylococci affect the ability of staphylococcus aureus to adhere to and internalize into bovine mammary epithelial cells
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10605872/
https://www.ncbi.nlm.nih.gov/pubmed/37884947
http://dx.doi.org/10.1186/s13567-023-01232-3
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