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Biochemical Properties of a Promising Milk-Clotting Enzyme, Moose (Alces alces) Recombinant Chymosin
Moose (Alces alces) recombinant chymosin with a milk-clotting activity of 86 AU/mL was synthesized in the Kluyveromyces lactis expression system. After precipitation with ammonium sulfate and chromatographic purification, a sample of genetically engineered moose chymosin with a specific milk-clottin...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606240/ https://www.ncbi.nlm.nih.gov/pubmed/37893665 http://dx.doi.org/10.3390/foods12203772 |
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author | Balabova, Dina V. Belash, Ekaterina A. Belenkaya, Svetlana V. Shcherbakov, Dmitry N. Belov, Alexander N. Koval, Anatoly D. Mironova, Anna V. Bondar, Alexander A. Volosnikova, Ekaterina A. Arkhipov, Sergey G. Sokolova, Olga O. Chirkova, Varvara Y. Elchaninov, Vadim V. |
author_facet | Balabova, Dina V. Belash, Ekaterina A. Belenkaya, Svetlana V. Shcherbakov, Dmitry N. Belov, Alexander N. Koval, Anatoly D. Mironova, Anna V. Bondar, Alexander A. Volosnikova, Ekaterina A. Arkhipov, Sergey G. Sokolova, Olga O. Chirkova, Varvara Y. Elchaninov, Vadim V. |
author_sort | Balabova, Dina V. |
collection | PubMed |
description | Moose (Alces alces) recombinant chymosin with a milk-clotting activity of 86 AU/mL was synthesized in the Kluyveromyces lactis expression system. After precipitation with ammonium sulfate and chromatographic purification, a sample of genetically engineered moose chymosin with a specific milk-clotting activity of 15,768 AU/mg was obtained, which was used for extensive biochemical characterization of the enzyme. The threshold of the thermal stability of moose chymosin was 55 °C; its complete inactivation occurred after heating at 60 °C. The total proteolytic activity of moose chymosin was 0.332 A(280) units. The ratio of milk-clotting and total proteolytic activities of the enzyme was 0.8. The K(m), k(cat) and k(cat)/K(m) values of moose chymosin were 4.7 μM, 98.7 s(−1), and 21.1 μM(−1) s(−1), respectively. The pattern of change in the coagulation activity as a function of pH and Ca(2+) concentration was consistent with the requirements for milk coagulants for cheese making. The optimum temperature of the enzyme was 50–55 °C. The introduction of Mg(2+), Zn(2+), Co(2+), Ba(2+), Fe(2+), Mn(2+), Ca(2+), and Cu(2+) into milk activated the coagulation ability of moose chymosin, while Ni ions on the contrary inhibited its activity. Using previously published data, we compared the biochemical properties of recombinant moose chymosin produced in bacterial (Escherichia coli) and yeast (K. lactis) producers. |
format | Online Article Text |
id | pubmed-10606240 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106062402023-10-28 Biochemical Properties of a Promising Milk-Clotting Enzyme, Moose (Alces alces) Recombinant Chymosin Balabova, Dina V. Belash, Ekaterina A. Belenkaya, Svetlana V. Shcherbakov, Dmitry N. Belov, Alexander N. Koval, Anatoly D. Mironova, Anna V. Bondar, Alexander A. Volosnikova, Ekaterina A. Arkhipov, Sergey G. Sokolova, Olga O. Chirkova, Varvara Y. Elchaninov, Vadim V. Foods Article Moose (Alces alces) recombinant chymosin with a milk-clotting activity of 86 AU/mL was synthesized in the Kluyveromyces lactis expression system. After precipitation with ammonium sulfate and chromatographic purification, a sample of genetically engineered moose chymosin with a specific milk-clotting activity of 15,768 AU/mg was obtained, which was used for extensive biochemical characterization of the enzyme. The threshold of the thermal stability of moose chymosin was 55 °C; its complete inactivation occurred after heating at 60 °C. The total proteolytic activity of moose chymosin was 0.332 A(280) units. The ratio of milk-clotting and total proteolytic activities of the enzyme was 0.8. The K(m), k(cat) and k(cat)/K(m) values of moose chymosin were 4.7 μM, 98.7 s(−1), and 21.1 μM(−1) s(−1), respectively. The pattern of change in the coagulation activity as a function of pH and Ca(2+) concentration was consistent with the requirements for milk coagulants for cheese making. The optimum temperature of the enzyme was 50–55 °C. The introduction of Mg(2+), Zn(2+), Co(2+), Ba(2+), Fe(2+), Mn(2+), Ca(2+), and Cu(2+) into milk activated the coagulation ability of moose chymosin, while Ni ions on the contrary inhibited its activity. Using previously published data, we compared the biochemical properties of recombinant moose chymosin produced in bacterial (Escherichia coli) and yeast (K. lactis) producers. MDPI 2023-10-13 /pmc/articles/PMC10606240/ /pubmed/37893665 http://dx.doi.org/10.3390/foods12203772 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Balabova, Dina V. Belash, Ekaterina A. Belenkaya, Svetlana V. Shcherbakov, Dmitry N. Belov, Alexander N. Koval, Anatoly D. Mironova, Anna V. Bondar, Alexander A. Volosnikova, Ekaterina A. Arkhipov, Sergey G. Sokolova, Olga O. Chirkova, Varvara Y. Elchaninov, Vadim V. Biochemical Properties of a Promising Milk-Clotting Enzyme, Moose (Alces alces) Recombinant Chymosin |
title | Biochemical Properties of a Promising Milk-Clotting Enzyme, Moose (Alces alces) Recombinant Chymosin |
title_full | Biochemical Properties of a Promising Milk-Clotting Enzyme, Moose (Alces alces) Recombinant Chymosin |
title_fullStr | Biochemical Properties of a Promising Milk-Clotting Enzyme, Moose (Alces alces) Recombinant Chymosin |
title_full_unstemmed | Biochemical Properties of a Promising Milk-Clotting Enzyme, Moose (Alces alces) Recombinant Chymosin |
title_short | Biochemical Properties of a Promising Milk-Clotting Enzyme, Moose (Alces alces) Recombinant Chymosin |
title_sort | biochemical properties of a promising milk-clotting enzyme, moose (alces alces) recombinant chymosin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606240/ https://www.ncbi.nlm.nih.gov/pubmed/37893665 http://dx.doi.org/10.3390/foods12203772 |
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