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Genome-Wide Detection of Copy Number Variations Associated with Miniature Features in Horses
Copy number variations (CNVs) are crucial structural genomic variants affecting complex traits in humans and livestock animals. The current study was designed to conduct a comprehensive comparative copy number variation analysis among three breeds, Debao (DB), Baise (BS), and Warmblood (WB), with a...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606273/ https://www.ncbi.nlm.nih.gov/pubmed/37895283 http://dx.doi.org/10.3390/genes14101934 |
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author | Choudhury, Md. Panir Wang, Zihao Zhu, Min Teng, Shaohua Yan, Jing Cao, Shuwei Yi, Guoqiang Liu, Yuwen Liao, Yuying Tang, Zhonglin |
author_facet | Choudhury, Md. Panir Wang, Zihao Zhu, Min Teng, Shaohua Yan, Jing Cao, Shuwei Yi, Guoqiang Liu, Yuwen Liao, Yuying Tang, Zhonglin |
author_sort | Choudhury, Md. Panir |
collection | PubMed |
description | Copy number variations (CNVs) are crucial structural genomic variants affecting complex traits in humans and livestock animals. The current study was designed to conduct a comprehensive comparative copy number variation analysis among three breeds, Debao (DB), Baise (BS), and Warmblood (WB), with a specific focus on identifying genomic regions associated with miniature features in horses. Using whole-genome next-generation resequencing data, we identified 18,974 CNVs across 31 autosomes. Among the breeds, we found 4279 breed-specific CNV regions (CNVRs). Baise, Debao, and Warmblood displayed 2978, 986, and 895 distinct CNVRs, respectively, with 202 CNVRs shared across all three breeds. After removing duplicates, we obtained 1545 CNVRs from 26 horse genomes. Functional annotation reveals enrichment in biological functions, including antigen processing, cell metabolism, olfactory conduction, and nervous system development. Debao horses have 970 genes overlapping with CNVRs, possibly causing their small size and mountainous adaptations. We also found that the genes GHR, SOX9, and SOX11 may be responsible for the miniature features of the Debao horse by analyzing their overlapping CNVRs. Overall, this study offers valuable insights into the widespread presence of CNVs in the horse genome. The findings contribute to mapping horse CNVs and advance research on unique miniature traits observed in the Debao horse. |
format | Online Article Text |
id | pubmed-10606273 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106062732023-10-28 Genome-Wide Detection of Copy Number Variations Associated with Miniature Features in Horses Choudhury, Md. Panir Wang, Zihao Zhu, Min Teng, Shaohua Yan, Jing Cao, Shuwei Yi, Guoqiang Liu, Yuwen Liao, Yuying Tang, Zhonglin Genes (Basel) Article Copy number variations (CNVs) are crucial structural genomic variants affecting complex traits in humans and livestock animals. The current study was designed to conduct a comprehensive comparative copy number variation analysis among three breeds, Debao (DB), Baise (BS), and Warmblood (WB), with a specific focus on identifying genomic regions associated with miniature features in horses. Using whole-genome next-generation resequencing data, we identified 18,974 CNVs across 31 autosomes. Among the breeds, we found 4279 breed-specific CNV regions (CNVRs). Baise, Debao, and Warmblood displayed 2978, 986, and 895 distinct CNVRs, respectively, with 202 CNVRs shared across all three breeds. After removing duplicates, we obtained 1545 CNVRs from 26 horse genomes. Functional annotation reveals enrichment in biological functions, including antigen processing, cell metabolism, olfactory conduction, and nervous system development. Debao horses have 970 genes overlapping with CNVRs, possibly causing their small size and mountainous adaptations. We also found that the genes GHR, SOX9, and SOX11 may be responsible for the miniature features of the Debao horse by analyzing their overlapping CNVRs. Overall, this study offers valuable insights into the widespread presence of CNVs in the horse genome. The findings contribute to mapping horse CNVs and advance research on unique miniature traits observed in the Debao horse. MDPI 2023-10-13 /pmc/articles/PMC10606273/ /pubmed/37895283 http://dx.doi.org/10.3390/genes14101934 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Choudhury, Md. Panir Wang, Zihao Zhu, Min Teng, Shaohua Yan, Jing Cao, Shuwei Yi, Guoqiang Liu, Yuwen Liao, Yuying Tang, Zhonglin Genome-Wide Detection of Copy Number Variations Associated with Miniature Features in Horses |
title | Genome-Wide Detection of Copy Number Variations Associated with Miniature Features in Horses |
title_full | Genome-Wide Detection of Copy Number Variations Associated with Miniature Features in Horses |
title_fullStr | Genome-Wide Detection of Copy Number Variations Associated with Miniature Features in Horses |
title_full_unstemmed | Genome-Wide Detection of Copy Number Variations Associated with Miniature Features in Horses |
title_short | Genome-Wide Detection of Copy Number Variations Associated with Miniature Features in Horses |
title_sort | genome-wide detection of copy number variations associated with miniature features in horses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606273/ https://www.ncbi.nlm.nih.gov/pubmed/37895283 http://dx.doi.org/10.3390/genes14101934 |
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